Anti-CAPZA1 抗体 [EPR11210-4R] (ab175233)
![Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)](https://www.abcam.co.jp/ps/products/175/ab175233/Images/ab175233-201608-ab175233.jpg "Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11210-4R] to CAPZA1
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CAPZA1 antibody [EPR11210-4R]
CAPZA1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR11210-4R] to CAPZA1 -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-P, WBmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Recombinant fragment within Human CAPZA1. The exact sequence is proprietary.
Database link: P52907 -
ポジティブ･コントロール
- HeLa, Jurkat, 293T and HepG2 cell lysates; Human muscle and Human urinary bladder carcinoma tissues.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR11210-4R -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab175233の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP |
1/10 - 1/100.
|
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
1/10000 - 1/50000. Predicted molecular weight: 33 kDa.
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| 特記事項 |
|---|
|
IP
1/10 - 1/100. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
1/10000 - 1/50000. Predicted molecular weight: 33 kDa. |
ターゲット情報
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機能
F-actin-capping proteins bind in a Ca(2+)-independent manner to the fast growing ends of actin filaments (barbed end) thereby blocking the exchange of subunits at these ends. Unlike other capping proteins (such as gelsolin and severin), these proteins do not sever actin filaments. -
配列類似性
Belongs to the F-actin-capping protein alpha subunit family. -
細胞内局在
Cytoplasm > cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 829 Human
- Entrez Gene: 12340 Mouse
- Entrez Gene: 691149 Rat
- Omim: 601580 Human
- SwissProt: P52907 Human
- SwissProt: P47753 Mouse
- SwissProt: B2GUZ5 Rat
- Unigene: 727604 Human
see all -
別名
- Cap Z antibody
- Cappa 1 antibody
- Cappa1 antibody
see all
画像
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Western blot - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)All lanes : Anti-CAPZA1 antibody [EPR11210-4R] (ab175233) at 1/10000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 33 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)](https://www.abcam.co.jp/ps/products/175/ab175233/Images/ab175233-201604-ab175233b.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)Immunohistochemical analysis of paraffin embedded Human muscle tissue labeling CAPZA1 with ab175233 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)](https://www.abcam.co.jp/ps/products/175/ab175233/Images/ab175233-201606-ab175233d.jpg)
Immunoprecipitation - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)Immunoprecipitation of CAPZA1 from HepG2 cell lysate using ab175233 at 1/10.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)](https://www.abcam.co.jp/ps/products/175/ab175233/Images/ab175233-201603-ab175233c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)Immunohistochemical analysis of paraffin embedded Human urinary bladder carcinoma tissue labeling CAPZA1 with ab175233 at 1/100.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)](https://www.abcam.co.jp/ps/products/175/ab175233/Images/ab175233-5-benefits-of-recombinant-antibodies.png)
Anti-CAPZA1 antibody [EPR11210-4R] (ab175233)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab175233 は論文での使用が確認できていません。