Name: Anti-CAP1 antibody [EPR8339(B)]
Brand: RabMAb
SKU: ab155079
Rating: 4 (5 reviews)

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Western blot - Anti-CAP1 antibody [EPR8339(B)] (ab155079)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8339(B)] to CAP1
Suitable for: Flow Cyt (Intra), WB, IHC-P
Reacts with: Human

Carrier Free

製品名

Anti-CAP1 antibody [EPR8339(B)]
CAP1 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR8339(B)] to CAP1
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-Pmore details
適用なし: ICC/IF or IP
種交差性

交差種: Human
交差が予測される動物種: Mouse, Rat
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- HeLa, 293T, A431, HepG2 and Caco-2 cell lysates; 293 cells; Prostate tissue.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

製品の状態

Liquid
保存方法

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR8339(B)
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-CAP1 antibody [EPR8339(B)] - BSA and Azide free (ab249174)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa whole cell lysate (ab29545)
Recombinant Protein
- Recombinant Human CAP1 protein (ab161016)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab155079の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB	(4)	1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

特記事項
Flow Cyt (Intra) 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

追加情報

Is unsuitable for ICC/IF or IP.

機能

Directly regulates filament dynamics and has been implicated in a number of complex developmental and morphological processes, including mRNA localization and the establishment of cell polarity.
配列類似性

Belongs to the CAP family.
Contains 1 C-CAP/cofactor C-like domain.
細胞内局在

Cell membrane.
Target information above from: UniProt accession Q01518 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 10487 Human
- Entrez Gene: 12331 Mouse
- Entrez Gene: 64185 Rat
- Omim: 601896 Human
- SwissProt: Q01518 Human
- SwissProt: P40124 Mouse
- SwissProt: Q08163 Rat
- Unigene: 370581 Human
- Unigene: 713078 Human
- Unigene: 8687 Mouse
- Unigene: 112585 Rat
see all
別名
- Adenylyl cyclase associated protein 1 antibody
- Adenylyl cyclase-associated protein 1 antibody
- CAP 1 antibody
- CAP antibody
- CAP, adenylate cyclase associated protein 1 (yeast) antibody
- CAP1 antibody
- CAP1 PEN antibody
- CAP1-PEN antibody
- CAP1_HUMAN antibody
see all

Western blot - Anti-CAP1 antibody [EPR8339(B)] (ab155079)

All lanes : Anti-CAP1 antibody [EPR8339(B)] (ab155079) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : Caco-2 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 52 kDa
Flow Cytometry (Intracellular) - Anti-CAP1 antibody [EPR8339(B)] (ab155079)

Intracellular flow cytometric analysis of permeabilized 293 cells, labeling CAP1 with ab155079 at 1/10 dilution (red) or Rabiit IgG negative control (green).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] (ab155079)

Immunohistochemical analysis of paraffin-embedded prostate tissue, labeling CAP1 with ab155079 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] (ab155079)

Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155079 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] (ab155079)

Immunohistochemical analysis of paraffin embedded Human gastric adenocarcinoma tissue using ab155079 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] (ab155079)

Immunohistochemical analysis of paraffin embedded Human normal liver tissue using ab155079 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAP1 antibody [EPR8339(B)] (ab155079)

Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155079 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-CAP1 antibody [EPR8339(B)] (ab155079)

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab155079 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab155079 は 5 報の論文で使用されています。

Hashimoto-Gotoh A et al. Persistent Infection of Simian Foamy Virus Derived from the Japanese Macaque Leads to the High-Level Expression of microRNA that Resembles the miR-1 microRNA Precursor Family. Microbes Environ 35:N/A (2020). PubMed: 31969530
Liu Y et al. Adenylyl cyclase-associated protein 1-targeted nanoparticles as a novel strategy for the treatment of metastatic non-small cell lung cancer. Int J Oncol 55:462-472 (2019). PubMed: 31173184
Sato H et al. Resistin upregulates chemokine production by fibroblast-like synoviocytes from patients with rheumatoid arthritis. Arthritis Res Ther 19:263 (2017). WB, IHC ; Human . PubMed: 29191223
Krochmal M et al. Identification of novel molecular signatures of IgA nephropathy through an integrative -omics analysis. Sci Rep 7:9091 (2017). PubMed: 28831120
Shao Z et al. LINGO-1 Regulates Oligodendrocyte Differentiation through the Cytoplasmic Gelsolin Signaling Pathway. J Neurosci 37:3127-3137 (2017). PubMed: 28193690

レビューと Q&A

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レビューを送る質問を送る

1-5 of 5 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (HUES7 EScells)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

HUES7 EScells

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 04 2016

Application

Western blot

Sample

Human Cell lysate - whole cell (HEPG2)

Gel Running Conditions

Reduced Denaturing (12.5)

Loading amount

20 µg

Specification

HEPG2

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 13 2015

Application

Western blot

Sample

Human Cell lysate - whole cell (MET5A)

Gel Running Conditions

Reduced Denaturing (10% gel)

Loading amount

100000 cells

Specification

MET5A

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 19 2015

Application

Western blot

Sample

Mouse Cell lysate - whole cell (mouse embryonic stem cell)

Gel Running Conditions

Reduced Denaturing (10% gel)

Loading amount

100000 cells

Specification

mouse embryonic stem cell

Blocking step

Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 20 2013

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Specification

HeLa

Fixative

Paraformaldehyde

Permeabilization

Yes - 0.5% Triton X-100 in PBS

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Kirk Mcmanus

Verified customer

投稿 Feb 21 2013

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (5)

レビューと Q&A

Western blot abreview for Anti-CAP1 antibody [EPR8339(B)]

Western blot abreview for Anti-CAP1 antibody [EPR8339(B)]

Western blot abreview for Anti-CAP1 antibody [EPR8339(B)]

Western blot abreview for Anti-CAP1 antibody [EPR8339(B)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-CAP1 antibody [EPR8339(B)]