Anti-Cannabinoid Receptor I 抗体 (ab40860)
Key features and details
- Goat polyclonal to Cannabinoid Receptor I
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cannabinoid Receptor I antibody
Cannabinoid Receptor I 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Cannabinoid Receptor I -
由来種
Goat -
特異性
This antibody is expected to recognise both reported isoforms (NP_057167.2 and NP_149421.1). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog
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免疫原
Synthetic peptide corresponding to Human Cannabinoid Receptor I aa 320-334 (internal sequence). (NP_057167.2; NP_001153732.1; NP_001153731.1; NP_001153730.1; NP_001153698.1; NP_149421.1)
Sequence:HTSEDGKVQVTRPDQ
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ポジティブ･コントロール
- WB: HeLa cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab40860の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 54, 63 kDa (predicted molecular weight: 53 kDa).
A 1 hour primary incubation is recommended for this product. The observed molecular weights correspond to the glycosylated (63 kDa) and unglycosylated (54 kDa) forms as described in the literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343). |
| 特記事項 |
|---|
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WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 54, 63 kDa (predicted molecular weight: 53 kDa). A 1 hour primary incubation is recommended for this product. The observed molecular weights correspond to the glycosylated (63 kDa) and unglycosylated (54 kDa) forms as described in the literature with different antibodies (Porcella et al, Eur J Neurosci. 2000 Mar;12(3):1123-7. PMID: 10762343). |
ターゲット情報
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機能
Involved in cannabinoid-induced CNS effects. Acts by inhibiting adenylate cyclase. Could be a receptor for anandamide. Inhibits L-type Ca(2+) channel current. Isoform 2 and isoform 3 have altered ligand binding. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 481913 Dog
- Entrez Gene: 1268 Human
- Entrez Gene: 12801 Mouse
- Entrez Gene: 25248 Rat
- Omim: 114610 Human
- SwissProt: P21554 Human
- SwissProt: P47746 Mouse
- SwissProt: P20272 Rat
see all -
別名
- CANN6 antibody
- Cannabinoid receptor 1 antibody
- CB-R antibody
see all
画像
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Western blot - Anti-Cannabinoid Receptor I antibody (ab40860)All lanes : Anti-Cannabinoid Receptor I antibody (ab40860) at 1 µg/ml
Lane 1 : HeLa lysate in RIPA buffer (non-glycosylated)
Lane 2 : HeLa lysate in RIPA buffer (non-glycosylated) with peptide at 0.5 µg/ml
Lane 3 : HeLa lysate in RIPA buffer (glycosylated)
Lane 4 : HeLa lysate in RIPA buffer (glycosylated) with peptide at 0.5 µg/ml
Lysates/proteins at 35 µg per lane.
Predicted band size: 53 kDaPrimary incubation 1 hour at room temperature. Detected by chemiluminescence.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab40860 は 2 報の論文で使用されています。
- Forte N et al. Orexin-A and endocannabinoids are involved in obesity-associated alteration of hippocampal neurogenesis, plasticity, and episodic memory in mice. Nat Commun 12:6137 (2021). PubMed: 34675233
- Kim J et al. Docosahexaenoyl ethanolamide improves glucose uptake and alters endocannabinoid system gene expression in proliferating and differentiating C2C12 myoblasts. Front Physiol 5:100 (2014). Flow Cyt ; Mouse . PubMed: 24711795