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Anti-CAMTA1 抗体 (ab64119)

Reviews (1)Q&A (3)References (2)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMTA1 antibody (ab64119)

    Key features and details

    • Rabbit polyclonal to CAMTA1
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-CAMTA1 antibody
      CAMTA1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to CAMTA1

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: IHC-Pmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Horse, Chicken, Dog

    • 免疫原

      Synthetic peptide conjugated to KLH derived from within residues 1650 to the C-terminus of Human CAMTA1.

      Immunogen の所有権に関して (Peptide available as ab66539.)

    • ポジティブ･コントロール

      • This antibody gave a positive signal in human hippocampus tissue sections.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • バッファー

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 98.98% PBS, 1% BSA

      Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab64119の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    特記事項
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    ターゲット情報

    • 関連性

      CAMTA1 is a transcription activator which is a putatuove tumor suppressor. It is thought to interact with calmodulin. Defects in CAMTA1 are detected in patients with oligodendroglioma and astrocytoma

    • 細胞内局在

      Nuclear

    • 参照データベース

      • 別名

        • Calmodulin-binding transcription activator 1 antibody
        • CANPMR antibody
        • CMTA1 antibody
        • KIAA0833 antibody

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMTA1 antibody (ab64119)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CAMTA1 antibody (ab64119)
        IHC image of CAMTA1 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64119, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      参考文献 (2)

      ab64119 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab64119 は 2 報の論文で使用されています。

      • Lee SJ  et al. Epithelioid hemangioendotheliomas with TFE3 gene translocations are compossible with CAMTA1 gene rearrangements. Oncotarget 7:7480-8 (2016). IHC . PubMed: 26840265
      • Yusifli Z & Kösemehmetoglu K CAMTA1 immunostaining is not useful in differentiating epithelioid hemangioendothelioma from its potential mimickers. Turk Patoloji Derg 30:159-65 (2014). PubMed: 25110239

      レビューと Q&A

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      1-4 of 4 Abreviews or Q&A

      Western blot abreview for Anti-CAMTA1 antibody

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (cortex, hippocampus, cerebellum)
      Loading amount
      100 µg
      Specification
      cortex, hippocampus, cerebellum
      Gel Running Conditions
      Non-reduced Denaturing (8% gel)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      投稿 Sep 18 2012

      Question

      We are looking for good antibody for anti-mouse CAMTA antibody. Anti-CAMTA1 antibody (ab64119) is designed to against human Camta1, but its is also predicted to work with Mouse. Could you tell us the seqence of synthetic peptide you used, so we can tell how much difference between human and mouse? Or just tell us if the peptide sequence you used for this antibody is completely conserved in human and mouse. Thanks.

      Read More
      Answer

      Thank you for contacting us. The immunogen sequence used to create this product is xxxxxxx. This sequence is completely conserved in mouse. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Use our products? Submit an Abreview. Earn rewards! https://www.abcam.com/abreviews

      Read More

      Question

      Thank you for this offer. I will take this offer. But the antibody will be purchased through our lab account. Do you need any proof of purchase?

      Read More
      Answer

      I am very pleased to hear you would like to accept our offer and test ab64119 in WB. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for WB] and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. The active code may be used by your lab and will not be redeemed through your account. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Question

      Thank youvery much for this important information. Could you tell me if you test this antibody by western blot? thanks

      Read More
      Answer

      Thank you very much for your interest in ab64119.

      To our knowledge, this has not been tested in Western Blot. Therefore, I can offer a discount off a future purchase if you buy ab64119 now, test it in Western blot and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

      If you are interested in this offer, please follow these steps:

      1. Reply to this e-mail to let me know that you would like to proceed and test ab64119 inWestern Blot. I will then send a discount code. This code must be issued before purchasing so please wait for my reply before ordering.

      2. Purchase ab64119 either by phone, fax, or online (www.abcam.com).

      3. Test it in WB.

      4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

      5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.
      Please remember that submission of the Abreview is sufficient for the discount code to become active.

      We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab64119 turns out to be unsuitable for Western Blot, you will still receive the discount on your next purchase after your Abreview has been submitted.

      Please let me know if you have any questions about this offer and I would be happy to help you further.

      The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com