Anti-CAMKIV 抗体 [EP2565AY] (ab68218)
![Western blot - Anti-CAMKIV antibody [EP2565AY] (ab68218)](https://www.abcam.co.jp/ps/products/68/ab68218/Images/ab68218-551214-anti-camkiv-antibody-ep2565ay-western-blot-human-jurkat-brain.jpg "Western blot - Anti-CAMKIV antibody [EP2565AY] (ab68218)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2565AY] to CAMKIV
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CAMKIV antibody [EP2565AY]
CAMKIV 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2565AY] to CAMKIV -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details
適用なし: Flow Cyt (Intra) -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- WB: Jurkat and Human brain cells. ICC/IF: HeLa cells.
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特記事項
This product has switched from a hybridoma to recombinant production method on 29th August 2024.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2565AY -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68218の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/50.
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| WB |
1/100000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
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| 特記事項 |
|---|
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ICC/IF
1/50. |
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WB
1/100000. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). |
ターゲット情報
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機能
Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory. -
組織特異性
Expressed in epithelial ovarian cancer tissue. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200. -
細胞内局在
Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 814 Human
- Omim: 114080 Human
- SwissProt: Q16566 Human
- Unigene: 591269 Human
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別名
- Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
- Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
- Brain Ca++-calmodulin dependent protein kinase type IV antibody
see all
画像
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Western blot - Anti-CAMKIV antibody [EP2565AY] (ab68218)All lanes : Anti-CAMKIV antibody [EP2565AY] (ab68218) at 1/10000 dilution
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate
Lane 2 : Human brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
Lane 1 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Lane 2 : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 52 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: Lane 1, 5 seconds; Lane 2, 20 seconds.
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![Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody [EP2565AY] (ab68218)](https://www.abcam.co.jp/ps/products/68/ab68218/Images/ab68218-551215-anti-camkiv-antibody-ep2565ay-immunocytochemistry-human-hela-cells.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CAMKIV antibody [EP2565AY] (ab68218)Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervical adenocarcinoma epithelial cell) cells labelling CAMKIV with ab68218 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) at 1/1000 dilution at RT for 45 min. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (ab195889) at 1/200 dilution, was co-incubated with ab68218 overnight at 4° C. Nucleus were visualized using DAPI.
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![Anti-CAMKIV antibody [EP2565AY] (ab68218)](https://www.abcam.co.jp/ps/products/68/ab68218/Images/ab68218-5-benefits-of-recombinant-antibodies.png)
Anti-CAMKIV antibody [EP2565AY] (ab68218)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab68218 は 1 報の論文で使用されています。
- Inagaki R et al. Kir6.1 Heterozygous Mice Exhibit Aberrant Amygdala-Dependent Cued Fear Memory. Mol Neurobiol 57:1622-1635 (2020). PubMed: 31808063