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  1. Link

    products/primary-antibodies/camkiv-antibody-ep2564y-ab75874.pdf

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Neuroscience Neurotransmission Calcium Signaling Calmodulin / CaMK
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RabMAb

Anti-CAMKIV 抗体 [EP2564Y] (ab75874)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (1)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-CAMKIV antibody [EP2564Y] (ab75874)

    Key features and details

    • Rabbit monoclonal [EP2564Y] to CAMKIV
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Conjugates logo Related conjugates and formulations

    Carrier Free

    こちらの製品もご検討ください

    一次抗体
    Product image
    Anti-GNAI1 antibody [EPR9441(B)] (ab140125)
    二次抗体
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    タンパク質
    Product image
    Recombinant Human CAMKIV protein (ab60756)

    関連製品

    製品の概要

    • 製品名

      Anti-CAMKIV antibody [EP2564Y]
      CAMKIV 一次抗体 製品一覧
    • 製品の詳細

      Rabbit monoclonal [EP2564Y] to CAMKIV
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: WBmore details
      適用なし: Flow Cyt,IHC-P or IP
    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat
    • 免疫原

      Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

    • ポジティブ・コントロール

      • WB: Jurkat whole cell lysate and Human brain lysate.
    • 特記事項

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • バッファー

      pH: 7.20
      Preservative: 0.01% Sodium azide
      Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
    • Concentration information loading...
    • 精製度

      Protein A purified
    • ポリ/モノ

      モノクローナル
    • クローン名

      EP2564Y
    • アイソタイプ

      IgG
    • 研究分野

      • Neuroscience
      • Neurotransmission
      • Calcium Signaling
      • Calmodulin / CaMK
      • Epigenetics and Nuclear Signaling
      • Chromatin Modifying Enzymes
      • Phosphorylation
      • Signal Transduction
      • Signaling Pathway
      • Calcium Signaling
      • Calmodulin Pathway

    関連製品

    • Isotype control

      • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Positive Controls

      • Jurkat cytoplasmic extract lysate (ab14842)
    • Recombinant Protein

      • Recombinant Human CAMKIV protein (ab60756)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab75874の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    1/1000 - 1/2000. Predicted molecular weight: 52 kDa.
    特記事項
    WB
    1/1000 - 1/2000. Predicted molecular weight: 52 kDa.
    追加情報
    Is unsuitable for Flow Cyt,IHC-P or IP.

    ターゲット情報

    • 機能

      Calcium/calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. May be involved in transcriptional regulation. May be involved in regulation of microtubule dynamics. In vitro, phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. May be involved in spermatogenesis. May play a role in the consolidation/retention of hippocampus-dependent long-term memory.
    • 組織特異性

      Expressed in epithelial ovarian cancer tissue.
    • 配列類似性

      Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
      Contains 1 protein kinase domain.
    • 翻訳後修飾

      Autophosphorylated and phosphorylated by CAMKK1 and CAMKK2 (By similarity). Dephosphorylated by serine/threonine protein phosphatase 2A, probably on Thr-200.
    • 細胞内局在

      Cytoplasm. Nucleus. Substantial localization in certain neuronal nuclei. In spermatids associated with chromatin and nuclear matrix.
    • Target information above from: UniProt accession Q16566 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 814 Human
      • Entrez Gene: 12326 Mouse
      • Entrez Gene: 25050 Rat
      • Omim: 114080 Human
      • SwissProt: Q16566 Human
      • SwissProt: P08414 Mouse
      • SwissProt: P13234 Rat
      • Unigene: 591269 Human
      • Unigene: 222329 Mouse
      • Unigene: 11046 Rat
      see all
    • 別名

      • Brain Ca(2+) calmodulin dependent protein kinase type 4 antibody
      • Brain Ca(2+) calmodulin dependent protein kinase type IV antibody
      • Brain Ca++-calmodulin dependent protein kinase type IV antibody
      • Calcium / calmodulin dependent protein kinase type 4 catalytic chain antibody
      • Calcium / calmodulin dependent protein kinase type IV catalytic chain antibody
      • Calcium/calmodulin dependent protein kinase IV antibody
      • Calcium/calmodulin dependent protein kinase type IV antibody
      • Calcium/calmodulin-dependent protein kinase type IV antibody
      • CAM kinase 4 antibody
      • CAM kinase GR antibody
      • CAM kinase IV antibody
      • CAM kinase-GR antibody
      • CaMK 4 antibody
      • CAMK GR antibody
      • CaMK IV antibody
      • Camk4 antibody
      • CaMKGR antibody
      • IV antibody
      • KCC4_HUMAN antibody
      • MGC36771 antibody
      see all

    画像

    • Western blot - Anti-CAMKIV antibody [EP2564Y] (ab75874)
      Western blot - Anti-CAMKIV antibody [EP2564Y] (ab75874)
      All lanes : Anti-CAMKIV antibody [EP2564Y] (ab75874) at 1/2000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Human brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size: 52 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    プロトコール

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (1)

    ab75874 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab75874 は 1 報の論文で使用されています。

    • Yan X  et al. CaMKII-Mediated CREB Phosphorylation Is Involved in Ca2+-Induced BDNF mRNA Transcription and Neurite Outgrowth Promoted by Electrical Stimulation. PLoS One 11:e0162784 (2016). WB, ICC/IF ; Rat . PubMed: 27611779

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    Thanks a lot for your answer it was really fast!
    We do agree to order and get discount for the antibodies you have suggested:

    CDC42 Binding Protein Kinase Beta antibody ab61328

    CNKR2 antibody ab64844

    NEK7 antibody ab80948

    MEK3 antibody ab5428

    BMX antibody ab73887

    Muscarinic Acetylcholine Receptor 1 antibody ab75178

    PPP4C antibody sb70888
    But I have one more question.
    On the https://www.abcam.com/index.html?pageconfig=resource&rid=11998 of your site in FAQ part, the last question isreferred to the antibodies that have already been tested in the applications for some species, but have no image. And the answer says that there is a possibility to get discount also for those antibodies.
    There are still 11 antibodies that have no images for the proposed species. if it is possible to give us some more discounts, we will submit the reviews including images for the proposed antibodies.
    Can you please have a look one more time?

    Thank you!
    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Mar 05 2012

    Answer

    Thank you for your reply.
    Reviewing the remaining antibodies that are already tested in WB and the species you wish to use, the following do not yet have a western blot image, and I would be pleased to arrange a testing discount for these additional two antibodies:
    ab13898
    ab28669
    I hope this will be helpful. I look forward to receiving your confirmation that you would like to go ahead, and I will be pleased to arrange and send the discount codes to you.
    For the remaining antibodies, I can suggest you may like to consider submitting an Abreview anyway, for which you can obtain Abpoints:
    ab85612
    ab75874
    ab61113
    ab47504
    ab16476
    ab85608
    ab31232
    ab41172
    ab23832
    Thank you for your time and continued interest in our products. I look forward to hearing from you.

    Read More

    Abcam Scientific Support

    Answered on Mar 05 2012

    Question

    Dear support team,
    We kindly appreciate the quality of your products and technical support!

    According to the rules of your 100% Testing Discount we would like to test a number of primary antibodies in Western blotting for Native and reduced conditions. The list of antibodies with catalog numbers and species is attached.

    As a permanent client of your company and active research team we will provide you with reliable reviews in short terms.
    Hope for a good cooperation.






    Best regards

    Read More

    Abcam community

    Verified customer

    Asked on Mar 02 2012

    Answer

    Thank you very much for your interest in our antibodies.

    We appreciate receiving feedback and data from customers, and we do have a testing discount offer available.

    If a customer would like to test an antibody in an application or species that it has not been previously tested in, we are able to provide our testing discount program.

    In this case, the antibodiess you have listed have all been tested in western blot already, but we would be able to provide this offer to you to test the following antibodies in mouse and rat:

    Discount to detect in mouse and rat (the following antibodies have not yet been tested in mouse or rat):
    CDC42 Binding Protein Kinase Beta antibody ab61328
    CNKR2 antibody ab64844
    NEK7 antibody ab80948

    Discount to test in mouse (the following antibodies have not yet been tested in mouse):
    MEK3 antibody ab5428
    BMX antibody ab73887
    Muscarinic Acetylcholine Receptor 1 antibody ab75178

    Discount to test in rat (the following antibodies have not been tested in rat):
    PPP4C antibody sb70888

    The following are already tested in western blot, and in mouse and/or rat as per your list. I am sorry we are therefore not able to provide a testing discount for these.
    ROCK2 antibody ab85612 Already tested WB, ms and rat
    CAMKIV antibody [EP2564Y] ab75874 Already tested WB, ms and rat
    FAK antibody ab61113 Already tested WB, ms and rat
    S6K antibody ab47504 Previously tested WB, ms and rat
    PPP1A antibody ab 16476 Previously tested WB, ms and rat
    5HT7 Receptor antibody ab13898 WB and mouse already tested.
    NMDAR1 antibody ab28669 Previously tested WB, ms and rat
    Dopamine Receptor D1 antibody ab85608 Previously tested WB, ms and rat
    Glutamate Receptor 1 (AMPA subtype) antibody ab31232 Previously tested WB and rat
    Nicotinic Acetylcholine Receptor alpha 4 antibody ab41172 Previously tested WB and mouse
    Nicotinic Acetylcholine Receptor alpha 7 antibody ab23832 Previously tested WB and rat

    For the antibodies we can provide a discount for, I can offer a discount off a future purchase if you buy the antibody(ies) now, test them in mouse, rat or both as indicated above, and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount provided for each individual antibody would be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and test these antibodies in mouse, rat or both. I will then send a discount code. This code must be issued before purchasing the antibodies so please wait for my reply before ordering.

    2. Purchase the antibodies either by phone, fax, or online (www.abcam.com).

    3. Test it in mouse, rat or both.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any [PRIMARY ANTIBODY /PROTEIN] ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for the species, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    Also, we are able to provide a discount for any order of 5 items or more. Therefore, once you have decided which antibodies you are going to purchase, please contact our customer service department before you place the order with a full list of the antibodies you wish to buy (orders@abcam.com). They will then be able to provide you with a quote.

    I hope this will be helpful to you. I look forward to receiving confirmation that you would like to receive the discount codes. If you have any further questions, please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Mar 02 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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