Anti-CaMKII (phospho T286) 抗体 (ab32678)
Key features and details
- Rabbit polyclonal to CaMKII (phospho T286)
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
-
製品名
Anti-CaMKII (phospho T286) antibody
CaMKII 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CaMKII (phospho T286) -
由来種
Rabbit -
特異性
This antibody is specific for the ~50 kDa alpha CaM Kinase II subunit and the ~60 kDa beta CaM Kinase II subunit phosphorylated at Thr286 in Western blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Rat
交差が予測される動物種: Chicken, Cow
-
免疫原
Synthetic peptide corresponding to Rat CaMKII (phospho T286).
-
ポジティブ･コントロール
- WB: Rat cortex lysate
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.50
Constituents: 0.238% HEPES, 50% Glycerol, 0.87% Sodium chloride, 0.01% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab32678の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (18) |
1/1000. Predicted molecular weight: 50 kDa. Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.
|
| 特記事項 |
|---|
|
WB
1/1000. Predicted molecular weight: 50 kDa. Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII. |
ターゲット情報
-
機能
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
-
参照データベース
- Entrez Gene: 374174 Chicken
- Entrez Gene: 374175 Chicken
- Entrez Gene: 422688 Chicken
- Entrez Gene: 423737 Chicken
- Entrez Gene: 282162 Cow
- Entrez Gene: 525416 Cow
- Entrez Gene: 530719 Cow
- Entrez Gene: 532713 Cow
see all -
別名
- Calcium/calmodulin dependent protein kinase II alpha antibody
- Calcium/calmodulin dependent protein kinase II beta antibody
- Calcium/calmodulin dependent protein kinase II delta antibody
see all
画像
-
Western blot - Anti-CaMKII (phospho T286) antibody (ab32678)All lanes : Anti-CaMKII (phospho T286) antibody (ab32678) at 1/1000 dilution
Lane 1 : Rat brain cortex lysate.
Lane 2 : Rat brain cortex lysate preincubated with lambda-phosphatase.
Lysates/proteins at 50 µg per lane.
Predicted band size: 50 kDa
Observed band size: 50,60 kDa why is the actual band size different from the predicted?
Predicted molecular weight: ~50 kDa for the alpha subunit and ~60 kDa for the beta subunit of CaMKII.
These images show the phosphospecificity of this antibody.
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (87)
ab32678 は 87 報の論文で使用されています。
- Haugsten Hansen M et al. CaMKII and reactive oxygen species contribute to early reperfusion arrhythmias, but oxidation of CaMKIIδ at methionines 281/282 is not a determining factor. J Mol Cell Cardiol 175:49-61 (2023). PubMed: 36528076
- Ying H et al. Colchicine attenuates the electrical remodeling of post-operative atrial fibrillation through inhibited expression of immune-related hub genes and stabilization of microtubules. Int J Biol Sci 19:2934-2956 (2023). PubMed: 37324937
- Ding Y et al. Electroacupuncture promotes neurogenesis in the dentate gyrus and improves pattern separation in an early Alzheimer's disease mouse model. Biol Res 56:65 (2023). PubMed: 38041203
- Lee DS et al. PDI augments kainic acid-induced seizure activity and neuronal death by inhibiting PP2A-GluA2-PICK1-mediated AMPA receptor internalization in the mouse hippocampus. Sci Rep 13:13927 (2023). PubMed: 37626185
- Tan SY et al. Neural mechanism underlies CYLD modulation of morphology and synaptic function of medium spiny neurons in dorsolateral striatum. Front Mol Neurosci 16:1107355 (2023). PubMed: 36846565