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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-CaMKII delta 抗体 [EPR13095] (ab181052)

Reviews (1) Submit a question References (30)
Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
  • Anti-CaMKII delta antibody [EPR13095] (ab181052)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13095] to CaMKII delta
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 APC Carrier Free PE

製品の概要

  • 製品名

    Anti-CaMKII delta antibody [EPR13095]
    CaMKII delta 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR13095] to CaMKII delta

  • 由来種

    Rabbit

  • 特異性

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: ICC/IF

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: HEK-293T, HAP1, SW480, A431 and HeLa whole cell lysate (ab150035); Mouse heart tissue lysate; Rat spleen tissue lysate. IHC-P: Human thyroid carcinoma, cardiac and skeletal muscle tissues.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab181052の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (1)
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
特記事項
WB
1/1000 - 1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
追加情報
Is unsuitable for ICC/IF.

ターゲット情報

  • 機能

    CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release.

  • 組織特異性

    Expressed in cardiac muscle and skeletal muscle. Isoform Delta 3, isoform Delta 2, isoform Delta 8 and isoform Delta 9 are expressed in cardiac muscle. Isoform Delta 11 is expressed in skeletal muscle.

  • 配列類似性

    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Calcium / calmodulin dependent protein kinase 2 delta antibody
    • Calcium / calmodulin dependent protein kinase II delta antibody
    • calcium/calmodulin-dependent protein kinase (CaM kinase) II delta antibody
    • calcium/calmodulin-dependent protein kinase type II delta chain antibody
    • Calcium/calmodulin-dependent protein kinase type II subunit delta antibody
    • CAM kinase 2 delta antibody
    • CAM kinase II delta antibody
    • CaM kinase II delta subunit antibody
    • CaM kinase II subunit delta antibody
    • CaM-kinase II delta chain antibody
    • CAMK 2d antibody
    • CaMK-II delta subunit antibody
    • CaMK-II subunit delta antibody
    • CAMK2D antibody
    • CAMKD antibody
    • CAMKI antibody
    • KCC2D_HUMAN antibody
    • RATCAMKI antibody
    see all

画像

  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    All lanes : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution

    Lane 1 : Wild-type HEK-293T cell lysate
    Lane 2 : CAMK2D knockout HEK-293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 56 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?



    Lanes 1- 2: Merged signal (red and green). Green - ab181052 observed at 50 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab181052 was shown to react with CaM-kinase II  in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab267322 (knockout cell lysate ab257376) was used. Wild-type HEK-293T and CAMK2D knockout HEK-293T cell lysates were subjected to SDS-PAGE. ab181052 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human thyroid carcinoma tissue sections labeling CaMKII delta with Purified ab181052 at 1:100 dilution (1.82 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: CaMKII delta knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: A431 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab181052 observed at 56 kDa. Red - loading control, ab8245, observed at 37 kDa.

    Unpurified ab181052 was shown to recognize CaMKII delta when CaMKII delta knockout samples were used, along with additional cross-reactive bands. Wild-type and CaMKII delta knockout samples were subjected to SDS-PAGE. ab181052 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Lane 1 : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution
    Lanes 2-4 : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/1000 dilution (purified)

    Lane 1 : HeLa ( Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg
    Lane 2 : SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 20 µg
    Lane 3 : Mouse heart lysates at 20 µg
    Lane 4 : Rat spleen lysates at 20 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa



    Blocking and diluting buffer : 5% NFDM/TBST

  • Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Western blot - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    All lanes : Anti-CaMKII delta antibody [EPR13095] (ab181052) at 1/2000 dilution (unpurified)

    Lane 1 : SW480 whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : HeLa whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle tissue sections labeling CaMKII delta with Purified ab181052 at 1:100 dilution (1.82 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CaMKII delta antibody [EPR13095] (ab181052)

    Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue sections labeling CaMKII delta using unpurified ab181052 at a 1/100 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Heat mediated antigen retrieval was performed citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-CaMKII delta antibody [EPR13095] (ab181052)
    Anti-CaMKII delta antibody [EPR13095] (ab181052)

参考文献 (30)

ab181052 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab181052 は 30 報の論文で使用されています。

  • Zhou D  et al. mROS‑calcium feedback loop promotes lethal ventricular arrhythmias and sudden cardiac death in early myocardial ischemia. Int J Mol Med 53:N/A (2024). PubMed: 37997788
  • Luo M  et al. Long-term potentiation and depression regulatory microRNAs were highlighted in Bisphenol A induced learning and memory impairment by microRNA sequencing and bioinformatics analysis. PLoS One 18:e0279029 (2023). PubMed: 36656826
  • Wang T  et al. Sequencing of the Pituitary Transcriptome after GnRH Treatment Uncovers the Involvement of lncRNA-m23b/miR-23b-3p/CAMK2D in FSH Synthesis and Secretion. Genes (Basel) 14:N/A (2023). PubMed: 37107604
  • Hsu YH  et al. Using brain cell-type-specific protein interactomes to interpret neurodevelopmental genetic signals in schizophrenia. iScience 26:106701 (2023). PubMed: 37207277
  • Liu L  et al. LCZ696 Ameliorates Tachycardia-Induced Cardiac Calcium Dyshomeostasis in the SERCA2α-Dependent Pathway. Tohoku J Exp Med 260:315-327 (2023). PubMed: 37258137
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

Western blot abreview for Anti-CaMKII delta antibody [EPR13095]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Renal - Including KO)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Specification
Mouse Renal - Including KO
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Edward Humphries

Verified customer

投稿 May 24 2017

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