Anti-CaMKII alpha 抗体 [6G9] (ab22609)
Key features and details
- Mouse monoclonal [6G9] to CaMKII alpha
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CaMKII alpha antibody [6G9]
CaMKII alpha 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [6G9] to CaMKII alpha -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Cow -
免疫原
Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human, rat, and mouse whole brain tissue lysates; IHC-P: Rat normal brain FFPE tissue sections.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
6G9 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab22609の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use at an assay dependent concentration.
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WB | (4) |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa). |
ターゲット情報
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機能
CaM-kinase II (CAMK2) is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses it may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
Contains 1 protein kinase domain. -
細胞内局在
Cell junction > synapse > presynaptic cell membrane. Cell junction > synapse. Postsynaptic lipid rafts. - Information by UniProt
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参照データベース
- Entrez Gene: 530719 Cow
- Entrez Gene: 815 Human
- Entrez Gene: 12322 Mouse
- Entrez Gene: 25400 Rat
- Omim: 114078 Human
- SwissProt: Q9UQM7 Human
- SwissProt: P11798 Mouse
- SwissProt: P11275 Rat
see all -
別名
- Alpha CaMKII antibody
- Calcium calmodulin dependent protein kinase II antibody
- Calcium/calmodulin dependent protein kinase II alpha B subunit antibody
see all
画像
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All lanes : Anti-CaMKII alpha antibody [6G9] (ab22609) at 10 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Brain (Human) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 65 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes -
IHC image of ab22609 staining in Rat normal brain (Purkinje cells, cerebellum) formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab22609, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (94)
ab22609 は 94 報の論文で使用されています。
- Natsubori A et al. Serotonergic neurons control cortical neuronal intracellular energy dynamics by modulating astrocyte-neuron lactate shuttle. iScience 26:105830 (2023). PubMed: 36713262
- Hou Y et al. Coordinated activity of a central pathway drives associative opioid analgesic tolerance. Sci Adv 9:eabo5627 (2023). PubMed: 36753548
- Inaba H et al. GPCR-mediated calcium and cAMP signaling determines psychosocial stress susceptibility and resiliency. Sci Adv 9:eade5397 (2023). PubMed: 37018397
- Chen A et al. Glycolysis mediates neuron specific histone acetylation in valproic acid-induced human excitatory neuron differentiation. Front Mol Neurosci 16:1151162 (2023). PubMed: 37089691
- Isoe Y et al. Epigenetically distinct synaptic architecture in clonal compartments in the teleostean dorsal pallium. Elife 12:N/A (2023). PubMed: 37489039