Anti-Calsequestrin 2 抗体 [EPR4228] (ab108381)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

Loading size & price…

Abpromise

保証された製品品質、優れたカスタマー・サポート。詳細はこちら。

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4228] to Calsequestrin 2
Suitable for: WB, IHC-P
Reacts with: Human

Carrier Free

製品名

Anti-Calsequestrin 2 antibody [EPR4228]
Calsequestrin 2 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR4228] to Calsequestrin 2
由来種

Rabbit
アプリケーション

適用あり: WB, IHC-Pmore details
種交差性

交差種: Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- Human heart and Human skeletal muscle lysates, Human cardiac muscles and Human skeletal muscles tissues
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR4228
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-Calsequestrin 2 antibody [EPR4228] - BSA and Azide free (ab247652)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human Calsequestrin 2 protein (ab93736)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab108381の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
WB		1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 46 kDa).
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 46 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.

機能

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium.
関連疾患

Defects in CASQ2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2) [MIM:611938]; also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT2 is an autosomal recessive form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death.
配列類似性

Belongs to the calsequestrin family.
細胞内局在

Sarcoplasmic reticulum lumen. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of cardiac and slow skeletal muscle cells.
Target information above from: UniProt accession O14958 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 845 Human
- Omim: 114251 Human
- SwissProt: O14958 Human
- Unigene: 57975 Human
別名
- AA033488 antibody
- AW146219 antibody
- Calsequestrin 2 (cardiac muscle) antibody
- Calsequestrin 2 fast twitch cardiac muscle antibody
- Calsequestrin antibody
- Calsequestrin cardiac muscle isoform antibody
- Calsequestrin fast twitch cardiac muscle antibody
- Calsequestrin-2 antibody
- Calsequestrin2 antibody
- cardCSQ antibody
- Cardiac calsequestrin 2 antibody
- cardiac muscle isoform antibody
- CASQ 2 antibody
- CASQ2 antibody
- CASQ2_HUMAN antibody
- cCSQ antibody
- ESTM52 antibody
- FLJ26321 antibody
- FLJ93514 antibody
- PDIB2 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscles using unpurified ab108381 at a dilution of 1/100.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Western blot - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

All lanes : Anti-Calsequestrin 2 antibody [EPR4228] (ab108381) at 1/10000 dilution (purified)

Lane 1 : Human fetal heart lysates
Lane 2 : Human skeletal muscle lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 46 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer: 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cardiac muscle tissue sections labeling Calsequestrin 2 with Purified ab108381 at 1:250 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
Western blot - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

All lanes : Anti-Calsequestrin 2 antibody [EPR4228] (ab108381) at 1/1000 dilution (unpurified)

Lane 1 : Human heart lysate
Lane 2 : Human skeletal muscle lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 46 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscles using unpurified ab108381 at a dilution of 1/100.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

Unpurified ab108381 showing negative staining in Normal brain tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

Unpurified ab108381 showing negative staining in Normal breast tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

Unpurified ab108381 showing negative staining in Normal kidney tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

Unpurified ab108381 showing negative staining in Normal colon tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

Unpurified ab108381 showing negative staining in Normal tonsil tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Anti-Calsequestrin 2 antibody [EPR4228] (ab108381)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

ab108381 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108381 は論文での使用が確認できていません。

レビューと Q&A

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Switch on our new BETA site

Now available

In the coming months

Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

関連疾患

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

SDS download

Datasheet download

参考文献 (0)

レビューと Q&A