Anti-Calsequestrin 2 抗体 [EPR4227] (ab108289)
![Western blot - Anti-Calsequestrin 2 antibody [EPR4227] (ab108289)](https://www.abcam.co.jp/ps/products/108/ab108289/Images/ab108289-120798-anti-calsequestrin-2-antibody-epr4227-western-blot.gif "Western blot - Anti-Calsequestrin 2 antibody [EPR4227] (ab108289)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4227] to Calsequestrin 2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-Calsequestrin 2 antibody [EPR4227]
Calsequestrin 2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR4227] to Calsequestrin 2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
ポジティブ･コントロール
- Human heart, mouse heart or rat heart lysates
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EPR4227 -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab108289の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 46 kDa).
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 46 kDa). |
追加情報
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
ターゲット情報
-
機能
Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. Binds 40 to 50 moles of calcium. -
関連疾患
Defects in CASQ2 are the cause of catecholaminergic polymorphic ventricular tachycardia type 2 (CPVT2) [MIM:611938]; also known as stress-induced polymorphic ventricular tachycardia (VTSIP). CPVT2 is an autosomal recessive form of arrhythmogenic disorder characterized by stress-induced, bidirectional ventricular tachycardia that may degenerate into cardiac arrest and cause sudden death. -
配列類似性
Belongs to the calsequestrin family. -
細胞内局在
Sarcoplasmic reticulum lumen. This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of cardiac and slow skeletal muscle cells. - Information by UniProt
-
参照データベース
- Entrez Gene: 845 Human
- Entrez Gene: 12373 Mouse
- Entrez Gene: 29209 Rat
- Omim: 114251 Human
- SwissProt: O14958 Human
- SwissProt: O09161 Mouse
- SwissProt: P51868 Rat
- Unigene: 57975 Human
see all -
別名
- AA033488 antibody
- AW146219 antibody
- Calsequestrin 2 (cardiac muscle) antibody
see all
画像
-
Western blot - Anti-Calsequestrin 2 antibody [EPR4227] (ab108289)All lanes : Anti-Calsequestrin 2 antibody [EPR4227] (ab108289) at 1/1000 dilution
Lane 1 : Human heart cell lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted? -
![Anti-Calsequestrin 2 antibody [EPR4227] (ab108289)](https://www.abcam.co.jp/ps/products/108/ab108289/Images/ab108289-2-benefits-of-recombinant-antibodies.png)
Anti-Calsequestrin 2 antibody [EPR4227] (ab108289)
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (8)
ab108289 は 8 報の論文で使用されています。
- Blackwell DJ et al. The Purkinje-myocardial junction is the anatomic origin of ventricular arrhythmia in CPVT. JCI Insight 7:N/A (2022). PubMed: 34990403
- Salazar-Enciso R et al. Aldosterone-Induced Sarco/Endoplasmic Reticulum Ca2+ Pump Upregulation Counterbalances Cav1.2-Mediated Ca2+ Influx in Mesenteric Arteries. Front Physiol 13:834220 (2022). PubMed: 35360237
- Kirsh AJ et al. Dietary nitrate does not alter cardiac function, calcium handling proteins, or SERCA activity in the left ventricle of healthy rats. Appl Physiol Nutr Metab N/A:N/A (2020). PubMed: 32379978
- Jannas-Vela S et al. Resting metabolic rate and skeletal muscle SERCA and Na+ /K+ ATPase activities are not affected by fish oil supplementation in healthy older adults. Physiol Rep 8:e14408 (2020). PubMed: 32342642
- Saadeh K et al. Protein expression profiles in murine ventricles modeling catecholaminergic polymorphic ventricular tachycardia: effects of genotype and sex. Ann N Y Acad Sci 1478:63-74 (2020). PubMed: 32713021
- Geng L et al. Rapid Electrical Stimulation Increased Cardiac Apoptosis Through Disturbance of Calcium Homeostasis and Mitochondrial Dysfunction in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Cell Physiol Biochem 47:1167-1180 (2018). PubMed: 29913448
- Estrada-Avilés R et al. The cardiac calsequestrin gene transcription is modulated at the promoter by NFAT and MEF-2 transcription factors. PLoS One 12:e0184724 (2017). PubMed: 28886186
- Curran J et al. EHD3-dependent endosome pathway regulates cardiac membrane excitability and physiology. Circ Res 115:68-78 (2014). PubMed: 24759929