Anti-Calreticulin 抗体 (ab18847)
Key features and details
- Rabbit polyclonal to Calreticulin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Calreticulin antibody
Calreticulin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calreticulin -
由来種
Rabbit -
特異性
Recognizes a 60 kDa calreticulin on SDS-PAGE immunoblots. Jurkat cell lysate can be used as a positive control.
-
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 3077 Calreticulin Antibody. 3077-100 is the same size as the 100 µg size of ab18847
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol (glycerin, glycerine), 0.5% BSA, 69% PBS -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab18847の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 48 kDa.
|
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 48 kDa. |
ターゲット情報
-
機能
Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. -
配列類似性
Belongs to the calreticulin family. -
ドメイン
Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.
The interaction with glycans occurs through a binding site in the globular lectin domain.
The zinc binding sites are localized to the N-domain.
Associates with PDIA3 through the tip of the extended arm formed by the P-domain. -
細胞内局在
Endoplasmic reticulum lumen. Cytoplasm > cytosol. Secreted > extracellular space > extracellular matrix. Cell surface. Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. - Information by UniProt
-
参照データベース
- Entrez Gene: 811 Human
- Omim: 109091 Human
- SwissProt: P27797 Human
- Unigene: 515162 Human
-
別名
- Autoantigen RO antibody
- CALR antibody
- CALR protein antibody
see all
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab18847 は 2 報の論文で使用されています。
- Kong Y et al. The Role of Neutrophil Extracellular Traps in Lipopolysaccharide-Induced Depression-like Behaviors in Mice. Brain Sci 11:N/A (2021). PubMed: 34827513
- Skardal A et al. Liver-Tumor Hybrid Organoids for Modeling Tumor Growth and Drug Response In Vitro. Ann Biomed Eng 43:2361-73 (2015). PubMed: 25777294