Anti-Calponin 2 抗体 (ab129331)
Key features and details
- Goat polyclonal to Calponin 2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Calponin 2 antibody -
製品の詳細
Goat polyclonal to Calponin 2 -
由来種
Goat -
特異性
This antibody is expected to recognize both reported isoforms (NP_004359.1; NP_958434.1), but it is not expected to cross-react with Calponin 3.
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Monkey, Gorilla, Orangutan
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免疫原
Synthetic peptide corresponding to Human Calponin 2 aa 292-306 (internal sequence). Near the C terminus of Human Calponin 2 (NP_004359.1).
Sequence:C-DPGEVPEYPPYYQEE
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ポジティブ･コントロール
- Human Placenta tissue; HepG2 lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab129331の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa).
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| IHC-P |
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 34 kDa). |
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IHC-P
Use a concentration of 2.5 - 3.75 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
組織特異性
Heart and smooth muscle. -
配列類似性
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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参照データベース
- Entrez Gene: 1265 Human
- Omim: 602373 Human
- SwissProt: Q99439 Human
- Unigene: 651512 Human
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別名
- Calponin 2 antibody
- Calponin H2 antibody
- Calponin H2, smooth muscle antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calponin 2 antibody (ab129331)
ab129331, at 2.5 µg/ml, staining Calponin 2 in formalin-fixed, paraffin-embedded Human Placenta tissue by Immunohistochemistry.
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Western blot - Anti-Calponin 2 antibody (ab129331)Anti-Calponin 2 antibody (ab129331) at 0.03 µg/ml + HepG2 lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 34 kDa
Primary incubation was 1 hour.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab129331 は論文での使用が確認できていません。