Anti-Calponin 1 抗体 [EP798Y] (ab46794)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP798Y] to Calponin 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human, Pig
Related conjugates and formulations
製品の概要
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製品名
Anti-Calponin 1 antibody [EP798Y]
Calponin 1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP798Y] to Calponin 1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat, Human, Pig
交差が予測される動物種: Sheep -
免疫原
Synthetic peptide within Human Calponin 1 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P51911 -
ポジティブ・コントロール
- ICC/IF: C2C12 and HeLa cells; Pig and mouse aortic smooth muscle cells. IHC-P: Rat lung tissue; Mouse cardiac muscle tissue; Human lung carcinoma, kidney, lung, tonsil, uterus, smooth muscle and skeletal muscle tissues. WB: Human bladder lysates, Pig heart lysates, Mouse bladder lysates and Rat bladder lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
解離定数(KD 値)
KD = 1.73 x 10 -10 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP798Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab46794の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (9) |
1/5000. Detects a band of approximately 34 kDa.
For unpurified use at 1/20,000. |
IHC-P | (3) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/100 - 1/250. |
ICC/IF | (4) |
1/500.
For unpurified use at 1/100 - 1/250. |
特記事項 |
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WB
1/5000. Detects a band of approximately 34 kDa. For unpurified use at 1/20,000. |
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/100 - 1/250. |
ICC/IF
1/500. For unpurified use at 1/100 - 1/250. |
ターゲット情報
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機能
Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. -
組織特異性
Smooth muscle, and tissues containing significant amounts of smooth muscle. -
配列類似性
Belongs to the calponin family.
Contains 3 calponin-like repeats.
Contains 1 CH (calponin-homology) domain. - Information by UniProt
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参照データベース
- Entrez Gene: 1264 Human
- Entrez Gene: 12797 Mouse
- Entrez Gene: 396911 Pig
- Entrez Gene: 65204 Rat
- Entrez Gene: 443517 Sheep
- Omim: 600806 Human
- SwissProt: P51911 Human
- SwissProt: Q08091 Mouse
see all -
別名
- Basic calponin antibody
- Calponin 1 antibody
- Calponin 1 basic smooth muscle antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence analysis of C2C12 (Mouse myoblasts myoblast) cells labeling Calponin 1 with purified ab46794 at 1/500 dilution. Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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All lanes : Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/50000 dilution (purified)
Lane 1 : Human bladder lysates
Lane 2 : Mouse bladder lysates
Lane 3 : Rat bladder lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilutionBlocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue sections labeling Calponin 1 with purified ab46794 at a 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used.
PBS instead of the primary antibody was used as the negative control (inset).
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All lanes : Anti-Calponin 1 antibody [EP798Y] (ab46794)
Lanes 1-2 : Non-Failing pig LV-myocardium tissue lysate.
Lanes 3-4 : Failing pig LV-myocardium tissue lysate injected with DOX.Ab46794 was used to stain Calponin 1 in Neonatal piglets injected with Dox (Failing LV myocardium) and PBS (Non-Failing LV myocardium). At the protein level a more than twofold increase in Calponin 1 was observed in Dox-injected animals compared to controls (PBS).
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Paraformadehyde-fixed, 0.25% Triton X-100 permeabilized mouse thoracic aortic smooth muscle cells labeling Calponin 1 using ab46794 at 1/100 dilution in ICC/IF, followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor 488) (ab150077) at 1/400 dilution.
1.5% BSA used used as blocking agent for 30 minutes at 25°C. Incubated with primary antibody for 24 hours at 4°C.
VSMCs were seeded to 35-mm plates in a low density avoiding overlapping of cells. After fixation, VSMCs were treated with 0.25% Triton X-100 for 20 minutes.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cardiac muscle tissue sections labeling Calponin 1 with purified ab46794 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used.
PBS instead of the primary antibody was used as the negative control (inset).
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Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/5000 dilution (purified) + Pig heart lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilutionBlocking and diluting buffer: 5% NFDM/TBST.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat lung tissue sections labeling Calponin 1 with purified ab46794 at 1:1000 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody.
PBS instead of the primary antibody was used as the negative control (inset).
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Unpurified ab46794 staining Calponin 1 in porcine aortic smooth muscle cells by Immunocytochemistry/ Immunofluorescence.
The cells were paraformaldehyde fixed, permeabilized in 0.1% Triton X-100. Samples were then incubated with primary antibody at 1/50 for 1 hour at 25°C. The secondary antibody used was ab6717 Goat polyclonal to Rabbit IgG - H&L (FITC) (green) used at a 1/400 dilution.
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Representative photomicrograph of mouse UT-myo cells (Left panel) and murine uterine myometrium (Right panel) stained with smooth muscle cell markers, alpha-SMA (red) and ab46794 (green) and DAPI (blue). UT-myo cells and whole-mount uterine tissue were collected from day 19 of mouse pregnancy. The placenta and embryo were removed from whole-mount tissue sections.
For full details please see paper.
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Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/20000 dilution (unpurified) + Human bladder lysate at 10 µg
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Observed band size: 34 kDa why is the actual band size different from the predicted? -
ICC/IF image of unpurified ab46794 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.
Cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab46794, 5µg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG - H&L, pre-adsorbed (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemical staining of paraffin-embedded human smooth muscle using unpurified ab46794 at 1/100 dilution
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All lanes : Anti-Calponin 1 antibody [EP798Y] (ab46794) at 1/20000 dilution (unpurified)
All lanes : Whole tissue lysate prepared from bovine lymph vessels
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit polyclonal at 1/5000 dilution
Developed using the ECL technique.
Observed band size: 34 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
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Unpurified ab46794 showing positive staining in normal lung vessel tissue.
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Unpurified ab46794 showing positive staining in normal kidney vessels tissue.
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Unpurified ab46794 showing positive staining in normal tonsil vessel tissue.
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Unpurified ab46794 showing positive staining in normal uterus tissue.
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Unpurified ab46794 showing negative staining in skeletal muscle tissue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (259)
ab46794 は 259 報の論文で使用されています。
- Liu S et al. Immunomodulatory hybrid micro-nanofiber scaffolds enhance vascular regeneration. Bioact Mater 21:464-482 (2023). PubMed: 36185748
- Zhang C et al. BRPF1 bridges H3K4me3 and H3K23ac in human embryonic stem cells and is essential to pluripotency. iScience 26:105939 (2023). PubMed: 36711238
- Zou M et al. Prdm6 drives ductus arteriosus closure by promoting ductus arteriosus smooth muscle cell identity and contractility. JCI Insight 8:N/A (2023). PubMed: 36749647
- Lou C & Li T Long non-coding RNA SENCR alleviates endothelial-to-mesenchymal transition via targeting miR-126a. Arch Med Sci 19:180-188 (2023). PubMed: 36817675
- Kim M et al. Differentiation of Adipose-Derived Stem Cells into Smooth Muscle Cells in an Internal Anal Sphincter-Targeting Anal Incontinence Rat Model. J Clin Med 12:N/A (2023). PubMed: 36836167