Anti-Calpain 2 抗体 (ab39165)
Key features and details
- Rabbit polyclonal to Calpain 2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Calpain 2 antibody
Calpain 2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calpain 2 -
由来種
Rabbit -
特異性
ab39165 binds to reduced and non-reduced calpain 2, but does not cross react with the other calpain family members (m calpain, calpain 94, ncl 2, ncl 3, etc.). It reacts with latent and active (amino-truncated) Calpain 2. -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide based on the amino terminal end of domain-III in the large subunit of Rat Calpain 2. (Peptide available as ab41302.)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab39165の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (6) |
1/1000 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 80 kDa). Dilution optimised using Chromogenic detection.
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| IHC-P |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 17192410
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| 特記事項 |
|---|
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WB
1/1000 - 1/5000. Detects a band of approximately 76 kDa (predicted molecular weight: 80 kDa). Dilution optimised using Chromogenic detection. |
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IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
Use at an assay dependent concentration. PubMed: 17192410 |
ターゲット情報
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機能
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 3 EF-hand domains. -
細胞内局在
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. - Information by UniProt
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参照データベース
- Entrez Gene: 824 Human
- Entrez Gene: 12334 Mouse
- Entrez Gene: 29154 Rat
- Omim: 114230 Human
- SwissProt: P17655 Human
- SwissProt: O08529 Mouse
- SwissProt: Q07009 Rat
- Unigene: 350899 Human
see all -
別名
- Calcium activated neutral proteinase 2 antibody
- Calcium activated neutral proteinase antibody
- Calcium-activated neutral protease 2, catalytic subunit antibody
see all
画像
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Western blot - Anti-Calpain 2 antibody (ab39165)
ab39165 was shown to react with CAPN2 in wild-type MDA-MB-231 (wpxld GFP) cells in Western blot with loss of signal observed in a CAPN2 knockout cell line. Wild-type MDA-MB-231 (wpxld GFP) and CAPN2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab39165 overnight at 4 °C at a 1/2000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2 μg/ml before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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Western blot - Anti-Calpain 2 antibody (ab39165)All lanes : Anti-Calpain 2 antibody (ab39165) at 1/1000 dilution
Lane 1 : Calpain 2 control
Lane 2 : Cell lysates from human chondrosarcoma (treated with IL1 alpha) at 10 µg
Performed under reducing conditions.
Predicted band size: 80 kDa
Observed band size: 76 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calpain 2 antibody (ab39165)ab39165 (4µg/ml) staining CALPAIN2 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (52)
ab39165 は 52 報の論文で使用されています。
- Lyu J & Ertbjerg P Sarcoplasmic and myofibril-bound calpains during storage of pork longissimus muscle: New insights on protein degradation. Food Chem 372:131347 (2022). PubMed: 34818749
- Yan J et al. Inherited Retinal Degeneration: PARP-Dependent Activation of Calpain Requires CNG Channel Activity. Biomolecules 12:N/A (2022). PubMed: 35327647
- Langlois B et al. LRP-1-dependent control of calpain expression and activity: A new mechanism regulating thyroid carcinoma cell adhesion. Front Oncol 12:981927 (2022). PubMed: 36052226
- Das S et al. Redefining the role of Ca2+-permeable channels in photoreceptor degeneration using diltiazem. Cell Death Dis 13:47 (2022). PubMed: 35013127
- Slater K et al. 1,4-dihydroxy quininib modulates the secretome of uveal melanoma tumour explants and a marker of oxidative phosphorylation in a metastatic xenograft model. Front Med (Lausanne) 9:1036322 (2022). PubMed: 36698840