Anti-Calpain 1 抗体 (ab28258)
Key features and details
- Rabbit polyclonal to Calpain 1
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Calpain 1 antibody
Calpain 1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calpain 1 -
由来種
Rabbit -
特異性
ab28258 recognizes Domain I of the large subunit of Calpain 1. The antibody does not recognize amino-processed Calpain 1, and can be used to discriminate between latent and amino-processed forms. Also ab28258 does not recognize the other calpain family members (M calpain, calpain 94, ncl 2, ncl 3, etc.). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human Calpain 1.
(Peptide available asab41299) -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody has been peptide-affinity purified. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab28258の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
1/1000 - 1/5000. Predicted molecular weight: 82 kDa. 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28258 identifies bands at 80 kDa, 68 kDa, and a series of further cleaved forms. Dilution optimised using Chromogenic detection.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 82 kDa. 1/1000, when using colorimetric substrates such as BCIP/NBT - 1/5000, when using chemiluminescent substrates. But note that when used against the reduced protein, ab28258 identifies bands at 80 kDa, 68 kDa, and a series of further cleaved forms. Dilution optimised using Chromogenic detection. |
ターゲット情報
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機能
Calcium-regulated non-lysosomal thiol-protease which catalyze limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the peptidase C2 family.
Contains 1 calpain catalytic domain.
Contains 4 EF-hand domains. -
細胞内局在
Cytoplasm. Cell membrane. Translocates to the plasma membrane upon Ca(2+) binding. - Information by UniProt
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別名
- Ca2 activated neutral protease antibody
- Calcium activated neutral proteinase antibody
- Calcium activated neutral proteinase small subunit antibody
see all
画像
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Anti-Calpain 1 antibody (ab28258) at 1/1000 dilution + Calpain 1 at 10 µg
Predicted band size: 82 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab28258 は 27 報の論文で使用されています。
- Wang WY et al. Calpain inhibitor MDL28170 alleviates cerebral ischemia‑reperfusion injury by suppressing inflammation and autophagy in a rat model of cardiac arrest. Exp Ther Med 25:196 (2023). PubMed: 37090078
- Gazorpak M et al. Harnessing PROTAC technology to combat stress hormone receptor activation. Nat Commun 14:8177 (2023). PubMed: 38071198
- Tang CK et al. Real-time monitoring of deformed wing virus-infected bee foraging behavior following histone deacetylase inhibitor treatment. iScience 24:103056 (2021). PubMed: 34755080
- Lu CW et al. Asiatic Acid Prevents Cognitive Deficits by Inhibiting Calpain Activation and Preserving Synaptic and Mitochondrial Function in Rats with Kainic Acid-Induced Seizure. Biomedicines 9:N/A (2021). PubMed: 33802221
- Sukocheva OA et al. Divergence of Intracellular Trafficking of Sphingosine Kinase 1 and Sphingosine-1-Phosphate Receptor 3 in MCF-7 Breast Cancer Cells and MCF-7-Derived Stem Cell-Enriched Mammospheres. Int J Mol Sci 22:N/A (2021). PubMed: 33919234