Anti-Calnexin (phospho S583) 抗体 (ab58503)
Key features and details
- Rabbit polyclonal to Calnexin (phospho S583)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Calnexin (phospho S583) antibody
Calnexin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calnexin (phospho S583) -
由来種
Rabbit -
特異性
ab58503 detects endogenous levels of Calnexin - ER membrane marker only when phosphorylated at serine 583. Recognizes ER membrane, mitochondria and cis-Golgi -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat
-
免疫原
Synthetic peptide corresponding to Human Calnexin aa 500-600 (phospho S583).
Database link: P27824 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
ab58503 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58503の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
|
| 特記事項 |
|---|
|
WB
1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
ターゲット情報
-
機能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
配列類似性
Belongs to the calreticulin family. -
細胞内局在
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
-
参照データベース
- Entrez Gene: 821 Human
- Entrez Gene: 29144 Rat
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35565 Rat
- Unigene: 567968 Human
- Unigene: 1762 Rat
-
別名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
画像
-
Western blot - Anti-Calnexin (phospho S583) antibody (ab58503)All lanes : Anti-Calnexin (phospho S583) antibody (ab58503) at 1/500 dilution
Lane 1 : HeLa cell extract treated with EGF (200ng/ml, 30mins)
Lane 2 : HeLa cell extract treated with EGF (200ng/ml, 30mins) with immunising peptide
Predicted band size: 90 kDa
Observed band size: 90 kDa
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab58503 は 2 報の論文で使用されています。
- Dallavilla T et al. Model-Driven Understanding of Palmitoylation Dynamics: Regulated Acylation of the Endoplasmic Reticulum Chaperone Calnexin. PLoS Comput Biol 12:e1004774 (2016). PubMed: 26900856
- Zhao Z TGFß and Wnt in Cardiac Outflow Tract Defects in Offspring of Diabetic Pregnancies. Birth Defects Res B Dev Reprod Toxicol 101:364-70 (2014). Mouse . PubMed: 25231192