Anti-Calnexin - ER membrane marker 抗体 - ER Marker (ab13504)
Key features and details
- Rabbit polyclonal to Calnexin - ER membrane marker - ER Marker
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Calnexin - ER membrane marker antibody - ER Marker
Calnexin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calnexin - ER membrane marker - ER Marker -
由来種
Rabbit -
特異性
Weak reactivity with Chicken, Fruitfly (Drosophila melanogaster) and Xenopus laevis. -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Dog Calnexin aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P24643 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13504の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | ||
| ICC/IF | (5) | |
| WB | (3) |
| 特記事項 |
|---|
ICC: 1/200.
ICC/IF: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution (PMID 18621691).
IP: 1/100.
WB: 1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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機能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
配列類似性
Belongs to the calreticulin family. -
細胞内局在
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Entrez Gene: 29144 Rat
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- SwissProt: P35565 Rat
- Unigene: 567968 Human
see all -
別名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)
Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed 2% Formaldehyde for 20 min at RT. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1/40000 for 2 hours at RT. (A) DAPI (blue) nuclear stain. (B) Anti-Calnexin-CT Antibody. (C) Composite. Heat Shocked at 42°C for 1h.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)Immunohistochemistry analysis using ab13504 at 1/100 for 1 hour at RT in mouse backskin. Fixed by Bouin’s Fixative Solution. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1/50 for 1 hour at RT.
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Western blot - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)All lanes : Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504) at 1/2000 dilution
All lanes : Mixture of rat tissue lysates
Predicted band size: 90 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/100 for 12 hours at 4°C in HaCaT cells. Fixed by Cold 100% methanol at -20C for 10 minutes. Secondary Antibody: FITC Goat Anti-Rabbit at 1/50 for 1-2 hours at RT in dark. /
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Immunocytochemistry/ Immunofluorescence - Anti-Calnexin - ER membrane marker antibody - ER Marker (ab13504)Immunocytochemistry/Immunofluorescence analysis using ab13504 at 1/80 for 12 hours at 4°C in Heat Shocked HeLa Cells. Fixed by 2% Formaldehyde for 20 min at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1/200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Heat Shocked at 42°C for 1h.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (36)
ab13504 は 36 報の論文で使用されています。
- Murakami A et al. Cell-autonomous control of intracellular temperature by unsaturation of phospholipid acyl chains. Cell Rep 38:110487 (2022). PubMed: 35294880
- Esfahanian N et al. Comprehensive Analysis of Proteasomal Complexes in Mouse Brain Regions Detects ENO2 as a Potential Partner of the Proteasome in the Striatum. Cell Mol Neurobiol 42:2305-2319 (2022). PubMed: 34037901
- Liu YY et al. Fatty Acid Synthase Is Involved in Classical Swine Fever Virus Replication by Interaction with NS4B. J Virol 95:e0078121 (2021). PubMed: 34132567
- Zhang L et al. S100A8 promotes chemoresistance via augmenting autophagy in B-cell lymphoma cells. Oncol Rep 45:151-158 (2021). PubMed: 33169801
- Li Y et al. MFSD7C switches mitochondrial ATP synthesis to thermogenesis in response to heme. Nat Commun 11:4837 (2020). PubMed: 32973183