Anti-Calnexin 抗体 - ER Membrane Marker (ab75801)
Key features and details
- Rabbit polyclonal to Calnexin - ER Membrane Marker
- Suitable for: ICC/IF, IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Calnexin antibody - ER Membrane Marker
Calnexin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calnexin - ER Membrane Marker -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, IP, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Dog Calnexin (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: P24643 -
特記事項
References regarding specificity
Horner SM et al. Mitochondrial-associated endoplasmic reticulum membranes (MAM) form innate immune synapses and are targeted by hepatitis C virus. Proc Natl Acad Sci U S A 108:14590-5 (2011). PubMed: 21844353
Myhill N et al. The subcellular distribution of calnexin is mediated by PACS-2. Mol Biol Cell 19:2777-88 (2008). PubMed: 18417615
Yoshimura SI et al. Direct targeting of cis-Golgi matrix proteins to the Golgi apparatus. J Cell Sci 114:4105-15 (2001). PubMed: 11739642
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75801の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100.
|
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| IHC-P |
1/100.
|
|
| IP |
1/100.
|
|
| WB |
1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
|
| 特記事項 |
|---|
|
ICC/IF
1/100. |
|
IHC-P
1/100. |
|
IP
1/100. |
|
WB
1/2000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
ターゲット情報
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機能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
配列類似性
Belongs to the calreticulin family. -
細胞内局在
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- Unigene: 567968 Human
- Unigene: 248827 Mouse
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別名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
画像
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Western blot - Anti-Calnexin antibody - ER Membrane Marker (ab75801)Anti-Calnexin antibody - ER Membrane Marker (ab75801) at 1/2000 dilution ((in TBS with 0.05% TW-20)) + HeLa cell lysate
Secondary
An HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 90 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody - ER Membrane Marker (ab75801)ab75801 staining Calnexin in juvenile mouse backskin tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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Immunoprecipitation - Anti-Calnexin antibody - ER Membrane Marker (ab75801)ab75801 used in Immunoprecipitation of Calnexin from Human Hela cells. The antibody was used at 20µl per 1 ml of cell lysate.
20µl of protein G beads were used in Immunoprecipitation.
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Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Membrane Marker (ab75801)ab75801 staining Calnexin in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rabbit IgG secondary antibody was used.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (68)
ab75801 は 68 報の論文で使用されています。
- Hong L et al. Uterine luminal-derived extracellular vesicles: potential nanomaterials to improve embryo implantation. J Nanobiotechnology 21:79 (2023). PubMed: 36882792
- Altamirano-Silva P et al. Phenotypes controlled by the Brucella abortus two component system BvrR/BvrS are differentially impacted by BvrR phosphorylation. Front Microbiol 14:1148233 (2023). PubMed: 37234533
- Zhao J et al. NETs Promote Inflammatory Injury by Activating cGAS-STING Pathway in Acute Lung Injury. Int J Mol Sci 24:N/A (2023). PubMed: 36982193
- Su H et al. Lipocalin 2 regulates mitochondrial phospholipidome remodeling, dynamics, and function in brown adipose tissue in male mice. Nat Commun 14:6729 (2023). PubMed: 37872178
- Saenz-de-Juano MD et al. Circadian Rhythm Does Not Affect the miRNA Cargo of Bovine Raw Milk Extracellular Vesicles. Int J Mol Sci 24:N/A (2023). PubMed: 37373358