Anti-Calnexin 抗体 (ab13505)
Key features and details
- Rabbit polyclonal to Calnexin
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Calnexin antibody
Calnexin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calnexin -
由来種
Rabbit -
特異性
Recognizes ER membrane, mitochondria and cis-Golgi -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide:
TAPPSSPKVTYKAPVPTGE
conjugated to KLH, corresponding to amino acids 50-68 of Human Calnexin -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13505の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100.
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ICC/IF |
1/100.
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WB |
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
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特記事項 |
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IHC-P
1/100. |
ICC/IF
1/100. |
WB
1/5000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa). |
ターゲット情報
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機能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
配列類似性
Belongs to the calreticulin family. -
細胞内局在
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 821 Human
- Entrez Gene: 12330 Mouse
- Entrez Gene: 29144 Rat
- Omim: 114217 Human
- SwissProt: P27824 Human
- SwissProt: P35564 Mouse
- SwissProt: P35565 Rat
- Unigene: 567968 Human
see all -
別名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
画像
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Anti-Calnexin antibody (ab13505) at 1/1000 dilution + Mixture of tissue lysates prepared from Rat tissues.
Predicted band size: 90 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (ab13505)
ab13505 staining Calnexin - ER membrane marker in mouse back skin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. The primary antibody was used at 1/100 dilution and a Fluorophore conjugated goat anti rabbit secondary at 1/50 dilution. 
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (ab13505)
ab13505 staining Calnexin - ER membrane marker in mouse colitis colon tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ was used for staining. A HRP-conjugated anti rabbit was used at 1/10 dilution as secondary.
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Anti-Calnexin antibody (ab13505) at 1/2000 dilution ((in TBS with 0.05% TW-20)) + HeLa cell lysate
Secondary
An HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 90 kDa -
ab13505 staining Calnexin in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rabbit IgG secondary antibody was used.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (5)
ab13505 は 5 報の論文で使用されています。
- Feng J et al. Human umbilical cord mesenchymal stem cells-derived exosomal circDLGAP4 promotes angiogenesis after cerebral ischemia-reperfusion injury by regulating miR-320/KLF5 axis. FASEB J 37:e22733 (2023). PubMed: 36723877
- Weghorst F et al. Caspase-3 Cleaves Extracellular Vesicle Proteins During Auditory Brainstem Development. Front Cell Neurosci 14:573345 (2020). PubMed: 33281555
- Rennoll-Bankert KE et al. Which Way In? The RalF Arf-GEF Orchestrates Rickettsia Host Cell Invasion. PLoS Pathog 11:e1005115 (2015). PubMed: 26291822
- Guziewicz KE et al. Molecular consequences of BEST1 gene mutations in canine multifocal retinopathy predict functional implications for human bestrophinopathies. Invest Ophthalmol Vis Sci 52:4497-505 (2011). PubMed: 21498618
- Grimsby JL et al. Role of lysyl oxidase propeptide in secretion and enzyme activity. J Cell Biochem 111:1231-43 (2010). WB . PubMed: 20717923