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AB13505

Anti-Calnexin 抗体

Anti-Calnexin antibody

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(5 Publications)

Rabbit Polyclonal Calnexin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Rat, Human, Mouse samples. Cited in 5 publications.

別名を表示する

Calnexin, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, CANX

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody (AB13505)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody (AB13505)

ab13505 staining Calnexin in HeLa cells by ICC/IF (Immunocytochemistry/immunofluoresence). Cells were fixed with 4% PFA for 20 minutes at room temperature. Samples were incubated with primary antibody (1/100) for 1 hour at room temperature. A Fluoroscein-conjugated goat anti-rabbit IgG secondary antibody was used.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (AB13505)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (AB13505)

ab13505 staining Calnexin - ER membrane marker in mouse colitis colon tissue section by immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. An antibody amplifier™ was used for staining. A HRP-conjugated anti rabbit was used at 1/10 dilution as secondary.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (AB13505)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody (AB13505)

ab13505 staining Calnexin - ER membrane marker in mouse back skin tissue section by Immunohistochemistry (Bouin's fixed paraffin embedded tissue sections). Tissue underwent heat mediated antigen retrieval in microwave with two, 5 minutes incubation intervals in citrate buffer. The primary antibody was used at 1/100 dilution and a Fluorophore conjugated goat anti rabbit secondary at 1/50 dilution.

Western blot - Anti-Calnexin antibody (AB13505)
  • WB

Unknown

Western blot - Anti-Calnexin antibody (AB13505)

All lanes:

Western blot - Anti-Calnexin antibody (ab13505) at 1/2000 dilution

All lanes:

HeLa cell lysate

Secondary

All lanes:

An HRP-conjugated goat anti-rabbit IgG

Predicted band size: 68 kDa

true

Western blot - Anti-Calnexin antibody (AB13505)
  • WB

Unknown

Western blot - Anti-Calnexin antibody (AB13505)

All lanes:

Western blot - Anti-Calnexin antibody (ab13505) at 1/1000 dilution

All lanes:

Mixture of tissue lysates prepared from Rat tissues.

Predicted band size: 68 kDa

false

Key facts

宿主種

Rabbit

クローン性

Polyclonal

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

IHC-P, WB, ICC/IF

applications

免疫原

Synthetic Peptide within Human CANX aa 50-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P27824

特異性

Recognizes ER membrane, mitochondria and cis-Golgi

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製度
Whole antiserum
バッファー組成
Constituents: Whole serum
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Calnexin also known as Canx is a type I integral membrane protein of the endoplasmic reticulum (ER) involved in the process of protein folding. This chaperone protein has an approximate molecular weight of 90 kDa and is known for its role in the quality control of glycoproteins. Calnexin is expressed in the ER of cells where it interacts with nascent polypeptides to ensure proper folding and assembly contributing to cellular homeostasis. It exhibits its function through its lectin-like domain that binds to sugar moieties on glycoproteins.
Biological function summary

Calnexin facilitates the proper folding of newly synthesized proteins by forming a complex with another chaperone protein called ERp57. This interaction helps in creating the correct disulfide bonds in glycoproteins which is essential for their stability and functionality. The complex often referred to as the calnexin cycle is critical in preventing the aggregation and misfolding of proteins within the ER. This process ensures that only correctly folded proteins proceed to the Golgi apparatus for further processing and transport.

Pathways

Calnexin plays an important role in the ER-associated degradation (ERAD) pathway and the unfolded protein response (UPR). In these pathways calnexin ensures that misfolded proteins are retained in the ER or targeted for degradation preventing cellular stress. Calnexin is associated with proteins such as calreticulin another chaperone protein with a similar function in the ER. Together they maintain proteostasis within cells and protect against the accumulation of improperly folded proteins.

Calnexin is linked to several conditions including cystic fibrosis and certain neurodegenerative diseases. In cystic fibrosis the misfolding and subsequent degradation of the CFTR protein are associated with calnexin's role in the ERAD pathway. Similarly in neurodegenerative diseases such as Alzheimer's disrupted protein folding and aggregation are linked to ER stress where calnexin and other chaperone proteins like BiP play a pivotal role in managing protein misfolding. Understanding calnexin's role in these disorders can contribute to developing strategies to mitigate faulty protein folding and its pathological consequences.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse.
See full target information CANX
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文献 (5)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22733 PubMed36723877

2023

Human umbilical cord mesenchymal stem cells-derived exosomal circDLGAP4 promotes angiogenesis after cerebral ischemia-reperfusion injury by regulating miR-320/KLF5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Feng,Wei He,Jian Xia,Qing Huang,Jie Yang,Wen-Ping Gu,Ning Zhang,Yun-Hai Liu

Frontiers in cellular neuroscience 14:573345 PubMed33281555

2020

Caspase-3 Cleaves Extracellular Vesicle Proteins During Auditory Brainstem Development.

Applications

Unspecified application

Species

Unspecified reactive species

Forrest Weghorst,Yeva Mirzakhanyan,Kian Samimi,Mehron Dhillon,Melanie Barzik,Lisa L Cunningham,Paul D Gershon,Karina S Cramer

PLoS pathogens 11:e1005115 PubMed26291822

2015

Which Way In? The RalF Arf-GEF Orchestrates Rickettsia Host Cell Invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Kristen E Rennoll-Bankert,M Sayeedur Rahman,Joseph J Gillespie,Mark L Guillotte,Simran J Kaur,Stephanie S Lehman,Magda Beier-Sexton,Abdu F Azad

Investigative ophthalmology & visual science 52:4497-505 PubMed21498618

2011

Molecular consequences of BEST1 gene mutations in canine multifocal retinopathy predict functional implications for human bestrophinopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Karina E Guziewicz,Julianna Slavik,Sarah J P Lindauer,Gustavo D Aguirre,Barbara Zangerl

Journal of cellular biochemistry 111:1231-43 PubMed20717923

2010

Role of lysyl oxidase propeptide in secretion and enzyme activity.

Applications

WB

Species

Unspecified reactive species

Jessica L Grimsby,Hector A Lucero,Philip C Trackman,Katya Ravid,Herbert M Kagan
View all publications
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代替製品

Primary Antibodies

AB213243

Anti-Calnexin antibody [EPR21205]

Recombinant|KO Validated

Western blot - Anti-Calnexin antibody [EPR21205] (AB213243)

  • 0
  • 0
Primary Antibodies

AB195198

HRP Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB195198)

  • 0
  • 0
Primary Antibodies

AB92573

Anti-Calnexin antibody [EPR3632]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR3632] (AB92573)

  • 0
  • 1
Primary Antibodies

AB133615

Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB133615)

  • 5
  • 1
Primary Antibodies

AB203439

Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 594 Anti-Calnexin antibody [EPR3633(2)] - ER Membrane Marker (AB203439)

  • 0
  • 0
Primary Antibodies

AB232433

Anti-Calnexin antibody [EPR3632] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody [EPR3632] - BSA and Azide free (AB232433)

  • 0
  • 1
Primary Antibodies

AB225542

Anti-Calnexin antibody [EPR3633(2)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Calnexin antibody [EPR3633(2)] - BSA and Azide free (AB225542)

  • 0
  • 0

組合せ製品

Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10
Primary Antibodies

AB134045

Anti-CD63 antibody [EPR5702] - Late Endosome Marker

RabMAb|Recombinant|KO Validated|Lab Essentials|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (AB134045)

  • 3
  • 6
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB236630

Anti-CD9 antibody [EPR23105-121]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD9 antibody [EPR23105-121] (AB236630)

  • 5
  • 4

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