Anti-Calnexin 抗体 (ab10286)
Key features and details
- Rabbit polyclonal to Calnexin
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Calnexin antibody
Calnexin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calnexin -
由来種
Rabbit -
特異性
Recognizes ER membrane, mitochondria and cis-Golgi -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment (His-tag) corresponding to Dog Calnexin aa 1-500. Expressed in E. coli. N-terminal ER luminal domain.
Database link: P24643 -
ポジティブ･コントロール
- WB: Human CEM T-lymphoblastoid leukemia cell lines. MCF cells. HeLa total cell lysate. HeLa membrane fraction. MDA-MB231 total cell lysate. MDA-MB231 membrane fraction.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Constituent: 99% Rabbit Serum -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10286の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (14) |
1/2000 - 1/5000. Predicted molecular weight: 67 kDa. 1/2000 - 1/5000. Predicted molecular weight: 67 kDa; however it does run on SDS-PAGE as an ~90kDa protein, this is mainly due to a number of negative charges at the C-terminus of the protein which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility.
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| 特記事項 |
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WB
1/2000 - 1/5000. Predicted molecular weight: 67 kDa. 1/2000 - 1/5000. Predicted molecular weight: 67 kDa; however it does run on SDS-PAGE as an ~90kDa protein, this is mainly due to a number of negative charges at the C-terminus of the protein which effects the SDS binding to the molecules and consequently its SDS-PAGE mobility. |
ターゲット情報
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機能
Calcium-binding protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. -
配列類似性
Belongs to the calreticulin family. -
細胞内局在
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 821 Human
- Omim: 114217 Human
- SwissProt: P27824 Human
- Unigene: 567968 Human
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別名
- Calnexin antibody
- CALX_HUMAN antibody
- CANX antibody
see all
画像
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Western blot - Anti-Calnexin antibody (ab10286)
Western blot probed with ab10286 at 1/1000.
Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines.
Western blot probed with ab10286 at 1/1000. Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines. Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.
Lane 2 - human CEM T-lymphoblastoid leukemia cell lines. -
Western blot - Anti-Calnexin antibody (ab10286)Anti-Calnexin antibody (ab10286) at 1/1000 dilution + MCF7 cells
Secondary
Goat anti rabbit at 1/10000 dilution
Predicted band size: 67 kDa -
Western blot - Anti-Calnexin antibody (ab10286)This image is courtesy of an Abreview submitted by Dr Neil TaylorAll lanes : Anti-Calnexin antibody (ab10286) at 1/4000 dilution
Lane 1 : HeLa (Total cell lysate)
Lane 2 : HeLa (Membrane fraction)
Lane 3 : HeLa (Cytoplasmic fraction)
Lane 4 : MDA-MB231 (Total cell lysate)
Lane 5 : MDA-MB231 (Membrane fraction)
Lane 6 : MDA-MB231 (Cytoplasmic fraction)
Secondary
All lanes : HRP conjugated donkey anti-rabbit
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 67 kDa
Observed band size: 67 kDa
Exposure time: 5 minutes
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Western blot - Anti-Calnexin antibody (ab10286)This image is courtesy of an anonymous Abreview.Anti-Calnexin antibody (ab10286) at 1/2000 dilution + HeLa whole cell lysate
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 67 kDa
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Western blot - Anti-Calnexin antibody (ab10286)This image is courtesy of an Abreview submitted by Mr. Yannick Waumans.All lanes : Anti-Calnexin antibody (ab10286) at 1/4000 dilution
Lane 1 : CHO membrane fraction
Lane 2 : CHO cytosolic fraction
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit Ig at 1/5000 dilution
Predicted band size: 67 kDa
データシートおよび資料
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Datasheet download
参考文献 (83)
ab10286 は 83 報の論文で使用されています。
- Aharon A et al. Associations of maternal and placental extracellular vesicle miRNA with preeclampsia. Front Cell Dev Biol 11:1080419 (2023). PubMed: 36910147
- Bauer FN et al. CD73 activity of mesenchymal stromal cell-derived extracellular vesicle preparations is detergent-resistant and does not correlate with immunomodulatory capabilities. Cytotherapy 25:138-147 (2023). PubMed: 36244910
- Hara M et al. PNPLA2 mobilizes retinyl esters from retinosomes and promotes the generation of 11-cis-retinal in the visual cycle. Cell Rep 42:112091 (2023). PubMed: 36763501
- Qian K et al. CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization. Nature 613:160-168 (2023). PubMed: 36477540
- Veerman RE et al. Antigen-Loaded Extracellular Vesicles Induce Responsiveness to Anti-PD-1 and Anti-PD-L1 Treatment in a Checkpoint Refractory Melanoma Model. Cancer Immunol Res 11:217-227 (2023). PubMed: 36546872
