Anti-Calnexin 抗体

• WB

AbReview21557****

Western blot - Anti-Calnexin antibody (AB10286)

All lanes:

Western blot - Anti-Calnexin antibody (ab10286) at 1/2000 dilution

All lanes:

HeLa whole cell lysate

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 68 kDa

true

This image is courtesy of an anonymous Abreview.

• WB

Unknown

Western blot - Anti-Calnexin antibody (AB10286)

Western blot probed with ab10286 at 1/1000.

Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines.
Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.

Western blot probed with ab10286 at 1/1000. Lane 1 – calnexin deficient human NKR T-lymphoblastoid leukemia cell lines. Lane 2 - human CEM T-lymphoblastoid leukemia cell lines.

All lanes:

Western blot - Anti-Calnexin antibody (ab10286)

Predicted band size: 68 kDa

false

• WB

Unknown

Western blot - Anti-Calnexin antibody (AB10286)

All lanes:

Western blot - Anti-Calnexin antibody (ab10286) at 1/1000 dilution

All lanes:

MCF7 cells

Secondary

All lanes:

Goat anti rabbit at 1/10000 dilution

Predicted band size: 68 kDa

false

• WB

AbReview24921****

Western blot - Anti-Calnexin antibody (AB10286)

All lanes:

Western blot - Anti-Calnexin antibody (ab10286) at 1/4000 dilution

Lane 1:

CHO membrane fraction

Lane 2:

CHO cytosolic fraction

Secondary

All lanes:

HRP-conjugated Goat anti-Rabbit Ig at 1/5000 dilution

Predicted band size: 68 kDa

false

This image is courtesy of an Abreview submitted by Mr. Yannick Waumans.

• WB

AbReview7867****

Western blot - Anti-Calnexin antibody (AB10286)

All lanes:

Western blot - Anti-Calnexin antibody (ab10286) at 1/4000 dilution

Lane 1:

HeLa (Total cell lysate)

Lane 2:

HeLa (Membrane fraction)

Lane 3:

HeLa (Cytoplasmic fraction)

Lane 4:

MDA-MB231 (Total cell lysate)

Lane 5:

MDA-MB231 (Membrane fraction)

Lane 6:

MDA-MB231 (Cytoplasmic fraction)

Secondary

All lanes:

HRP conjugated donkey anti-rabbit

Predicted band size: 68 kDa

Observed band size: 67 kDa

true

Exposure time: 5min

This image is courtesy of an Abreview submitted by Dr Neil Taylor

• WB

CiteAb

Western blot - Anti-Calnexin antibody (AB10286)

Western Blotting using Anti-Calnexin antibody, ab10286. Publication image from Sánchez-Madrid, F. et al., 2016, Nat Commun, 27882925. Legend direct from paper.

ISGylation inhibits exosome secretion.(a) Western blot analysis of EVs purified by serial ultracentrifugation from cell culture supernatants from equal numbers of Jurkat T cells untreated (Cont) or treated with 1,000 U ml−1 IFN-I for 16 h. Cells and EVs (Exo) were blotted for the exosomal markers CD63, TSG101, Flotillin and CD81, and for the endoplasmic reticulum marker Calnexin. Right graph : quantification of exosomal protein levels in the EVs obtained from IFN-I-treated and untreated cells in three independent experiments. (b) Western blot analysis of the EVs obtained from equal numbers of untransfected HEK293 cells (Cont) or co-transfected with ISG15 and the ISGylation machinery; E1, E2, E3 ligases (ISG15). Cells and EVs (Exo) were blotted for CD63, TSG101, CD81 and Calnexin. Right graph : quantification of exosomal protein levels in the EVs obtained from untransfected HEK293 cells or co-transfected with ISG15 and the ISGylation machinery in three independent experiments. (c) Western blot analysis of the EVs obtained from equal numbers of HEK293 cells co-transfected with plasmids encoding the ISGylation machinery and the functional (ISG15WT) or mutated ISG15 (ISG15MUT). Cells and EVs (Exo) were blotted for CD63, CD81 and Calnexin. Right graph : quantification of exosomal protein levels in four independent experiments. (d) Western blot analysis of the EVs obtained from equal numbers of WT, ISG15KO and USP18C61A BMDMs treated 16 h with IFN-I or left untreated. Cells and EVs were blotted for TSG101 and quantification of exosomal protein levels of IFN-I-treated and -untreated cells is shown for three independent experiments. (e) Western blot analysis of the EVs obtained in blood serum from poly(I : C)-injected WT, ISG15KO and USP18C61A mice. EVs were isolated from 250 µl of serum and blotted for TSG101. Right graphs : quantification of exosomal TSG101 protein levels of three mice per genotype; t-test *P-value<0.05, **P-value<0.001 and ***P-value<0.0001.

false

• WB

CiteAb

Western blot - Anti-Calnexin antibody (AB10286)

Western Blotting using Anti-Calnexin antibody, ab10286. Publication image from Sánchez-Madrid, F. et al., 2016, Nat Commun, 27882925. Legend direct from paper.

ISGylation inhibits exosome secretion.(a) Western blot analysis of EVs purified by serial ultracentrifugation from cell culture supernatants from equal numbers of Jurkat T cells untreated (Cont) or treated with 1,000 U ml−1 IFN-I for 16 h. Cells and EVs (Exo) were blotted for the exosomal markers CD63, TSG101, Flotillin and CD81, and for the endoplasmic reticulum marker Calnexin. Right graph : quantification of exosomal protein levels in the EVs obtained from IFN-I-treated and untreated cells in three independent experiments. (b) Western blot analysis of the EVs obtained from equal numbers of untransfected HEK293 cells (Cont) or co-transfected with ISG15 and the ISGylation machinery; E1, E2, E3 ligases (ISG15). Cells and EVs (Exo) were blotted for CD63, TSG101, CD81 and Calnexin. Right graph : quantification of exosomal protein levels in the EVs obtained from untransfected HEK293 cells or co-transfected with ISG15 and the ISGylation machinery in three independent experiments. (c) Western blot analysis of the EVs obtained from equal numbers of HEK293 cells co-transfected with plasmids encoding the ISGylation machinery and the functional (ISG15WT) or mutated ISG15 (ISG15MUT). Cells and EVs (Exo) were blotted for CD63, CD81 and Calnexin. Right graph : quantification of exosomal protein levels in four independent experiments. (d) Western blot analysis of the EVs obtained from equal numbers of WT, ISG15KO and USP18C61A BMDMs treated 16 h with IFN-I or left untreated. Cells and EVs were blotted for TSG101 and quantification of exosomal protein levels of IFN-I-treated and -untreated cells is shown for three independent experiments. (e) Western blot analysis of the EVs obtained in blood serum from poly(I : C)-injected WT, ISG15KO and USP18C61A mice. EVs were isolated from 250 µl of serum and blotted for TSG101. Right graphs : quantification of exosomal TSG101 protein levels of three mice per genotype; t-test *P-value<0.05, **P-value<0.001 and ***P-value<0.0001.

false

• WB

CiteAb

Western blot - Anti-Calnexin antibody (AB10286)

Western Blotting using Anti-Calnexin antibody, ab10286. Publication image from Sánchez-Madrid, F. et al., 2016, Nat Commun, 27882925. Legend direct from paper.

ISGylation inhibits exosome secretion.(a) Western blot analysis of EVs purified by serial ultracentrifugation from cell culture supernatants from equal numbers of Jurkat T cells untreated (Cont) or treated with 1,000 U ml−1 IFN-I for 16 h. Cells and EVs (Exo) were blotted for the exosomal markers CD63, TSG101, Flotillin and CD81, and for the endoplasmic reticulum marker Calnexin. Right graph : quantification of exosomal protein levels in the EVs obtained from IFN-I-treated and untreated cells in three independent experiments. (b) Western blot analysis of the EVs obtained from equal numbers of untransfected HEK293 cells (Cont) or co-transfected with ISG15 and the ISGylation machinery; E1, E2, E3 ligases (ISG15). Cells and EVs (Exo) were blotted for CD63, TSG101, CD81 and Calnexin. Right graph : quantification of exosomal protein levels in the EVs obtained from untransfected HEK293 cells or co-transfected with ISG15 and the ISGylation machinery in three independent experiments. (c) Western blot analysis of the EVs obtained from equal numbers of HEK293 cells co-transfected with plasmids encoding the ISGylation machinery and the functional (ISG15WT) or mutated ISG15 (ISG15MUT). Cells and EVs (Exo) were blotted for CD63, CD81 and Calnexin. Right graph : quantification of exosomal protein levels in four independent experiments. (d) Western blot analysis of the EVs obtained from equal numbers of WT, ISG15KO and USP18C61A BMDMs treated 16 h with IFN-I or left untreated. Cells and EVs were blotted for TSG101 and quantification of exosomal protein levels of IFN-I-treated and -untreated cells is shown for three independent experiments. (e) Western blot analysis of the EVs obtained in blood serum from poly(I : C)-injected WT, ISG15KO and USP18C61A mice. EVs were isolated from 250 µl of serum and blotted for TSG101. Right graphs : quantification of exosomal TSG101 protein levels of three mice per genotype; t-test *P-value<0.05, **P-value<0.001 and ***P-value<0.0001.

false