Anti-Calcium Pump PMCA3 ATPase 抗体 (ab154213)
Key features and details
- Rabbit polyclonal to Calcium Pump PMCA3 ATPase
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-Calcium Pump PMCA3 ATPase antibody
Calcium Pump PMCA3 ATPase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Calcium Pump PMCA3 ATPase -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
-
免疫原
Recombinant fragment, corresponding to a region within internal sequence amino acids 560-887 of Human Calcium Pump PMCA3 ATPase (Uniprot ID: Q16720).
-
ポジティブ･コントロール
- U-87 MG whole cell lysate; Human U87 xenograft tissue.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab154213の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 134 kDa.
|
|
| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
| 特記事項 |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 134 kDa. |
|
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
-
関連性
Calcium Pump PMCA3 ATPase belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. Calcium Pump PMCA3 ATPase is the plasma membrane calcium ATPase isoform 3. -
細胞内局在
Cell membrane; Multi-pass membrane protein. -
参照データベース
- Entrez Gene: 504353 Cow
- Entrez Gene: 492 Human
- Entrez Gene: 320707 Mouse
- Entrez Gene: 29599 Rat
- Omim: 300014 Human
- SwissProt: Q16720 Human
- SwissProt: A2ALL9 Mouse
- SwissProt: Q0VF54 Mouse
see all -
別名
- ATP2B3 antibody
- ATP2B3 protein antibody
- ATPase Ca++ transporting plasma membrane 3 antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calcium Pump PMCA3 ATPase antibody (ab154213)Immunohistochemical analysis of paraffin-embedded Human U87 xenograft tissue, labeling Calcium Pump PMCA3 ATPase with ab154213 at 1/500 dilution.
-
Western blot - Anti-Calcium Pump PMCA3 ATPase antibody (ab154213)Anti-Calcium Pump PMCA3 ATPase antibody (ab154213) at 1/1000 dilution + U-87 MG whole cell lysate at 30 µg
Predicted band size: 134 kDa
5% SDS PAGE
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab154213 は論文での使用が確認できていません。