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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Calcineurin A 抗体 [EPR1670(2)] (ab109412)

Submit a review Submit a question References (6)
Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
  • Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
  • Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
  • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
  • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
  • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
  • Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1670(2)] to Calcineurin A
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 647 Carrier Free

製品の概要

  • 製品名

    Anti-Calcineurin A antibody [EPR1670(2)]
    Calcineurin A 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR1670(2)] to Calcineurin A

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WB, ICC/IFmore details
    適用なし: IHC-P or IP

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Fetal brain, SH-SY5Y, A431, and HeLa cell lysates; HeLa cells. Mouse and rat brain cortex lysates.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109412の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa).
ICC/IF
1/100 - 1/250.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/10000 - 1/50000. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa).
ICC/IF
1/100 - 1/250.
追加情報
Is unsuitable for IHC-P or IP.

ターゲット情報

  • 機能

    Calcium-dependent, calmodulin-stimulated protein phosphatase. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1.

  • 配列類似性

    Belongs to the PPP phosphatase family. PP-2B subfamily.

  • 細胞内局在

    Nucleus. Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Alpha isoform formerly PPP2B antibody
    • Calcineurin A alpha antibody
    • Calcineurin A1 antibody
    • CalcineurinA antibody
    • Calmodulin dependent calcineurin A subunit alpha isoform antibody
    • Calmodulin-dependent calcineurin A subunit alpha isoform antibody
    • CALN antibody
    • CALNA 1 antibody
    • CALNA antibody
    • CALNA1 antibody
    • CAM PRP catalytic subunit antibody
    • CAM-PRP catalytic subunit antibody
    • CCN 1 antibody
    • CCN1 antibody
    • CNA 1 antibody
    • CNA alpha antibody
    • CNA antibody
    • CNA1 antibody
    • PP2BA_HUMAN antibody
    • PPP2B antibody
    • Ppp3ca antibody
    • Protein phosphatase 2B catalytic subunit antibody
    • Protein phosphatase 3 (formerly 2B) catalytic subunit alpha isoform antibody
    • Protein phosphatase 3 catalytic subunit alpha isoform PPP3CA antibody
    • Protein phosphatase 3 catalytic subunit alpha isozyme antibody
    • Serine/threonine protein phosphatase 2B catalytic subunit alpha isoform antibody
    • Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform antibody
    see all

画像

  • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
    Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
    All lanes : Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) at 1/10000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : PPP3CA knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 59 kDa
    Observed band size: 59 kDa



    Lanes 1- 2: Merged signal (red and green). Green - ab109412 observed at 59 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab109412 was shown to react with Calcineurin A in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265130 (knockout cell lysate ab257181) was used. Wild-type HeLa and PPP3CA knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109412 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
    Immunocytochemistry/ Immunofluorescence - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
    Immunofluorescent staining of HeLa cells using 1/100 ab109412.
  • Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
    Flow Cytometry (Intracellular) - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)

    Overlay histogram showing HeLa cells stained with ab109412 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109412, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

     

  • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
    Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
    All lanes : Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) at 1/3000 dilution

    Lane 1 : Mouse brain cortex lysate
    Lane 2 : Mouse lung lysate
    Lane 3 : Rat brain cortex lysate
    Lane 4 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 dilution

    Predicted band size: 59 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?

  • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
    Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Calcineurin A knockout HAP1 cell lysate (20 µg)
    Lane 3: A431 cell lysate (20 µg)
    Lane 4: HeLa cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109412 observed at 60 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109412 was shown to specifically react with Calcineurin A when Calcineurin A knockout samples were used. Wild-type and Calcineurin A knockout samples were subjected to SDS-PAGE. ab109412 and ab8245 (loading control to GAPDH) were both diluted 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
    Western blot - Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
    All lanes : Anti-Calcineurin A antibody [EPR1670(2)] (ab109412) at 1/10000 dilution

    Lane 1 : fetal brain lysate
    Lane 2 : SH-SY5Y lysate
    Lane 3 : A431 lysate
    Lane 4 : HeLa cells lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 59 kDa
    Observed band size: 58 kDa why is the actual band size different from the predicted?

  • Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)
    Anti-Calcineurin A antibody [EPR1670(2)] (ab109412)

参考文献 (6)

ab109412 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109412 は 6 報の論文で使用されています。

  • Han QT  et al. The toxic natural product tutin causes epileptic seizures in mice by activating calcineurin. Signal Transduct Target Ther 8:101 (2023). PubMed: 36894540
  • Li L  et al. Calf Thymus Polypeptide Restrains the Growth of Colorectal Tumor via Regulating the Intestinal Microbiota-Mediated Immune Function. Front Pharmacol 13:898906 (2022). PubMed: 35662701
  • Moon JH  et al. Calcineurin Activation by Prion Protein Induces Neurotoxicity via Mitochondrial Reactive Oxygen Species. Oxid Med Cell Longev 2021:5572129 (2021). PubMed: 34394828
  • Gu LF  et al. Huangkui Capsule Ameliorates Renal Fibrosis in a Unilateral Ureteral Obstruction Mouse Model Through TRPC6 Dependent Signaling Pathways. Front Pharmacol 11:996 (2020). PubMed: 32719603
  • Liu Z  et al. Discovery of Small-Molecule Inhibitors of the HSP90-Calcineurin-NFAT Pathway against Glioblastoma. Cell Chem Biol 26:352-365.e7 (2019). PubMed: 30639261
  • Tian CJ & Pang X Ca2+-calcineurin signaling is involved in norepinephrine-induced cardiac fibroblasts activation. Int J Clin Exp Pathol 8:5210-6 (2015). PubMed: 26191219

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