Anti-Calcineurin A 抗体

• WB

Unknown

Western blot - Anti-Calcineurin A antibody (AB3673)

All lanes:

Western blot - Anti-Calcineurin A antibody (ab3673) at 1/200 dilution

Lane 1:

Mouse brain tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 59 kDa

Observed band size: 62 kDa

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• WB

Unknown

Western blot - Anti-Calcineurin A antibody (AB3673)

Western Blot of wild type mouse brain tissue homogenate with ab3673 used at 1/6000. Please note that this image was assembled from two different blots. (The numbers on the scale correspond to kD).

All lanes:

Western blot - Anti-Calcineurin A antibody (ab3673)

Predicted band size: 59 kDa

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• WB

AbReview12127****

Western blot - Anti-Calcineurin A antibody (AB3673)

All lanes:

Western blot - Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution

All lanes:

Huh7 (Human hepatocellular carcinoma) whole cell lysate at 15000 Cells

Secondary

All lanes:

HRP conjugated sheep polyclonal to rabbit IgG at 1/15000 dilution

Predicted band size: 59 kDa

Observed band size: 62 kDa

false

Exposure time: 1min

This image is a courtesy of Anonymous Abreview

• WB

Unknown

Western blot - Anti-Calcineurin A antibody (AB3673)

All lanes:

Western blot - Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human Calcineurin A protein (<a href='/products/proteins-peptides/recombinant-human-calcineurin-a-protein-ab101870'>ab101870</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 59 kDa

true

Exposure time: 10s

• WB

Unknown

Western blot - Anti-Calcineurin A antibody (AB3673)

All lanes:

Western blot - Anti-Calcineurin A antibody (ab3673) at 1/1000 dilution

All lanes:

Western blot - Recombinant Human Calcineurin A protein (<a href='/products/proteins-peptides/recombinant-human-calcineurin-a-protein-ab101870'>ab101870</a>) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 59 kDa

true

Exposure time: 10s

• WB

CiteAb

Western blot - Anti-Calcineurin A antibody (AB3673)

Calcineurin A western blot using anti-Calcineurin A antibody ab3673. Publication image and figure legend from Shinde, V., Kotla, P., et al., 2016, Cell Death Dis, PubMed 26844699.

ab3673 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab3673 please see the product overview.

The tunicamycin treatment of primary photoreceptor cultures and the subretinal injection of tunicamycin in SD rats result in cell death by the activation of calpain-induced Ca2+ signaling. (a–c) Primary photoreceptor cultured cells were confirmed to express rod photoreceptor markers such as rhodopsin (lines 1 and 2) and to lack cone and ganglia cell markers such as GNAT2 and Thy-1 compared with whole retinal cell lysate (line 3) and negative controls (line 4). (a, b) Different concentrations of Tn were used to verify a dose of Tn at which a rod cell viability would be compromised measured by MTT assay (N=6). The read absorbance correlated with viable cells. (c) The dose of 2 μg/ml Tn was used to test whether calpain activation occurred in Tn-treated rod photoreceptor cells (N=3). Increase in calpain activity was found 18 h after treatment with Tn (p<0.01). (d–i) (N=4). The dose of 0.02 μg/eye of Tn was used to inject SD retinas and then rat retinas were analyzed 4 days after injection (p<0.05 for all analyzed proteins compared to PBS injection). (d) The Tn injection resulted in the elevation of the calpain level. (e) In addition, an increase in the IP3R protein level was associated with Tn injection vs PBS-injected retinas. (f) This increase was in agreement with the elevated BI-1 protein and was opposite to the result of concomitant elevation of SERCA2b compared to PBS-treated retinas. (g, h) Interestingly, the calcineurin protein level was also significantly increased in Tn-injected retinas. (i) Images of western blots treated with correspondent antibodies are shown. Data are shown as means±S.E.M. Images of western blots treated with a correspondent antibody are shown above the graphs for the calculation of individual proteins. *,***p<0.01

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