Anti-Calbindin 抗体 [EP3478]
Anti-Calbindin antibody [EP3478]
- BOND RX™ Validated
- 20ul selling size
- RabMAb
- Advanced Validation
- Recombinant
- 詳細を見る
4
(3 Reviews)
|
(29 Publications)
Anti-Calbindin antibody [EP3478] (ab108404) is a rabbit monoclonal antibody detecting Calbindin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, mIHC. Suitable for Human, Mouse, Rat.
- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
別名を表示する
CAB27, CALB1, Calbindin, Calbindin D28, D-28K
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (AB108404)
Immunofluorescent analysis was performed on 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse hippocampal primary neural/glia cells labelling Calbindin with ab108404 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody (ab150077) at dilution of 1/1000 (green).
Anti-MAP2 antibody [HM-2] (ab11267) at 1/1000 dilution was used as a counterstain, with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed as the secondary antibody (ab150120) at dilution of 1/1000 (red).
Negative control 1 : Anti-Calbindin (ab108404) at 1/250 dilution with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed as the secondary antibody (ab150120) at dilution of 1/1000.
Negative control 2 : Anti-MAP2 antibody [HM-2] (ab11267) at 1/200 dilution with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody (ab150077) at dilution of 1/1000.
The nuclear counterstain is DAPI (blue).
Confocal image showing positive staining in subsets of mouse hippocampal primary neuron.
Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
- WB
Unknown
Western blot - Anti-Calbindin antibody [EP3478] (AB108404)
All lanes:
Western blot - Anti-Calbindin antibody [EP3478] (ab108404) at 1/1000 dilution
Lane 1:
Fetal brain lysate at 10 µg
Lane 2:
Fetal cerebellum lysate at 10 µg
Lane 3:
Fetal hypothalamus lysate at 10 µg
Lane 4:
Fetal pituitary lysate at 10 µg
Predicted band size: 30 kDa
false
- WB
Lab
Western blot - Anti-Calbindin antibody [EP3478] (AB108404)
All lanes:
Western blot - Anti-Calbindin antibody [EP3478] (ab108404) at 1/2000 dilution
Lane 1:
Human fetal brain lysates at 20 µg
Lane 2:
SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg
Lane 3:
Human kidney lysates at 20 µg
Lane 4:
Mouse brain lysates at 20 µg
Lane 5:
Mouse kidney lysates at 20 µg
Lane 6:
Rat brain lysates at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Predicted band size: 30 kDa
Observed band size: 30 kDa
false
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
ab108404 showing negative staining in Normal colon tissue.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Calbindin with purified ab108404 at 1/1000 dilution (1.49 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-Calbindin antibody [EP3478] (AB108404)
Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Calbindin with purified ab108404 at 1/150 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
- IHC-Fr
Unknown
Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (AB108404)
Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling Calbindin with purified ab108404 at 1/500 dilution (3.0 µg/ml). The tissue section was fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.
Secondary antibody only control : PBS instead of the primary antibody
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
ab108404, at 1/100 dilution, staining Calbindin in paraffin-embedded Human brain tissue by Immunohistochemistry.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
ab108404 showing negative staining in Normal liver tissue.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
ab108404 showing negative staining in Normal heart tissue.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
ab108404 showing positive staining in Normal kidney tissue.
- IHC-Fr
Lab
Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (AB108404)
Immunohistochemical analysis of mouse frozen cerebellum tissue sections with ab108404 labelling Calbindin at 1/500. Sections were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). The image shows specific staining on Purkinje cells of mouse cerebellum.
Secondary antibody only was used as negative control and is shown in the inset.
- IHC-Fr
Unknown
Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (AB108404)
Immunohistochemistry (Frozen) analysis of rat cerebellum tissue sections labeling Calbindin with purified ab108404 at 1/500 dilution (3.0 µg/ml). The tissue section was fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.
Secondary antibody only control : PBS instead of the primary antibody
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Reactivity data
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製品の詳細
What is this antibody validated in?
Anti-Calbindin antibody [EP3478] (ab108404) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.
What is the molecular weight of Calbindin?
Anti-Calbindin [EP3478] (ab108404) specifically detects a band for Calbindin (UniProt: P05937) at a molecular weight of 30kDa.
Trusted by the scientific community
Anti-Calbindin [EP3478] (ab108404) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EP3478] also available for your convenience: ab108404, Alexa Fluor® 647 - ab207528, Alexa Fluor® 488 - ab208377, Carrier free - ab233018, PE - ab303059, APC - ab303060, HRP - ab303061, Alkaline Phosphatase - ab308740, Alexa Fluor® 594 - ab310455, Alexa Fluor® 555 - ab311984, Alexa Fluor® 568 - ab312459, Alexa Fluor® 750 - ab320901
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
出荷温度及び保存条件
製品の状態
精製方法
バッファー組成
出荷温度
短期保存温度
長期保存温度
保管に関する情報
補足情報
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Calbindin contributes to cellular homeostasis by managing intracellular calcium levels. It does not appear to be part of a larger protein complex but operates independently. This protein helps to protect neurons from calcium overload potentially safeguarding against excitotoxicity which occurs when neurons die from excessive stimulation. Calbindin's function as a calcium buffer is of particular importance in maintaining neuron function and survival in areas with high neuronal activity.
Pathways
Calbindin has a significant role in the calcium signaling pathway and is also involved in the vitamin D pathway. The calcium signaling pathway is vital for many cellular processes like muscle contraction neurotransmitter release and gene expression. The vitamin D pathway regulates calcium and phosphate homeostasis in the body. Calbindin's relationship with proteins such as calmodulin and parvalbumin both involved in calcium regulation highlights its importance in maintaining cellular functions.
製品プロトコール
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ターゲットの情報
文献 (29)
Recent publications for all applications. Explore the full list and refine your search
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