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AB108404

Anti-Calbindin 抗体 [EP3478]

Anti-Calbindin antibody [EP3478]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Advanced Validation
  • Recombinant
  • 詳細を見る

4

(3 Reviews)

|

(29 Publications)

Anti-Calbindin antibody [EP3478] (ab108404) is a rabbit monoclonal antibody detecting Calbindin in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, ICC/IF, mIHC. Suitable for Human, Mouse, Rat.

- Multiplex IHC validated on the Leica BOND® MAX using Opal reagents
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications

別名を表示する

CAB27, CALB1, Calbindin, Calbindin D28, D-28K

13 Images
Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (AB108404)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Calbindin antibody [EP3478] (AB108404)

Immunofluorescent analysis was performed on 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse hippocampal primary neural/glia cells labelling Calbindin with ab108404 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody (ab150077) at dilution of 1/1000 (green).

Anti-MAP2 antibody [HM-2] (ab11267) at 1/1000 dilution was used as a counterstain, with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed as the secondary antibody (ab150120) at dilution of 1/1000 (red).

Negative control 1 : Anti-Calbindin (ab108404) at 1/250 dilution with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed as the secondary antibody (ab150120) at dilution of 1/1000.

Negative control 2 : Anti-MAP2 antibody [HM-2] (ab11267) at 1/200 dilution with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody (ab150077) at dilution of 1/1000.

The nuclear counterstain is DAPI (blue).

Confocal image showing positive staining in subsets of mouse hippocampal primary neuron.

Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Western blot - Anti-Calbindin antibody [EP3478] (AB108404)
  • WB

Unknown

Western blot - Anti-Calbindin antibody [EP3478] (AB108404)

All lanes:

Western blot - Anti-Calbindin antibody [EP3478] (ab108404) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Fetal cerebellum lysate at 10 µg

Lane 3:

Fetal hypothalamus lysate at 10 µg

Lane 4:

Fetal pituitary lysate at 10 µg

Predicted band size: 30 kDa

false

Western blot - Anti-Calbindin antibody [EP3478] (AB108404)
  • WB

Lab

Western blot - Anti-Calbindin antibody [EP3478] (AB108404)

All lanes:

Western blot - Anti-Calbindin antibody [EP3478] (ab108404) at 1/2000 dilution

Lane 1:

Human fetal brain lysates at 20 µg

Lane 2:

SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg

Lane 3:

Human kidney lysates at 20 µg

Lane 4:

Mouse brain lysates at 20 µg

Lane 5:

Mouse kidney lysates at 20 µg

Lane 6:

Rat brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 30 kDa

Observed band size: 30 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)

ab108404 showing negative staining in Normal colon tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cerebrum tissue sections labeling Calbindin with purified ab108404 at 1/1000 dilution (1.49 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Flow Cytometry (Intracellular) - Anti-Calbindin antibody [EP3478] (AB108404)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Calbindin antibody [EP3478] (AB108404)

Intracellular Flow Cytometry analysis of SH-SY5Y (Human neuroblastoma epithelial cell) cells labeling Calbindin with purified ab108404 at 1/150 dilution (10 μg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (AB108404)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (AB108404)

Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling Calbindin with purified ab108404 at 1/500 dilution (3.0 µg/ml). The tissue section was fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.
Secondary antibody only control : PBS instead of the primary antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)

ab108404, at 1/100 dilution, staining Calbindin in paraffin-embedded Human brain tissue by Immunohistochemistry.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)

ab108404 showing negative staining in Normal liver tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)

ab108404 showing negative staining in Normal heart tissue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [EP3478] (AB108404)

ab108404 showing positive staining in Normal kidney tissue.

Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (AB108404)
  • IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (AB108404)

Immunohistochemical analysis of mouse frozen cerebellum tissue sections with ab108404 labelling Calbindin at 1/500. Sections were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue). The image shows specific staining on Purkinje cells of mouse cerebellum.

Secondary antibody only was used as negative control and is shown in the inset.

Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (AB108404)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Calbindin antibody [EP3478] (AB108404)

Immunohistochemistry (Frozen) analysis of rat cerebellum tissue sections labeling Calbindin with purified ab108404 at 1/500 dilution (3.0 µg/ml). The tissue section was fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. DAPI (blue) was used as nuclear counterstain.
Secondary antibody only control : PBS instead of the primary antibody

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  • 565 Alexa Fluor® 555

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  • 665 Alexa Fluor® 647

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  • 775 Alexa Fluor® 750

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Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EP3478

アイソタイプ

IgG

キャリアフリー

No

交差種

Mouse, Rat, Human

アプリケーション

ICC/IF, Flow Cyt (Intra), WB, IHC-Fr, mIHC, IHC-P

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

We do not guarantee IHC-P for mouse and rat.

We do not guarantee IHC-Fr for human.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/1000", "mIHC-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p><p>We do not guarantee IHC-P for mouse and rat.</p><p><strong>For unpurified use at 1/100 - 1/250.</strong></p>" }, "Mouse": { "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/150", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/100</strong></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>We do not guarantee IHC-Fr for human</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p><p>We do not guarantee IHC-P for mouse and rat.</p><p><strong>For unpurified use at 1/100 - 1/250.</strong></p>" }, "Rat": { "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "<p>We do not guarantee IHC-Fr for human</p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><a href='/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p><p>We do not guarantee IHC-P for mouse and rat.</p><p><strong>For unpurified use at 1/100 - 1/250.</strong></p>" } } }

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製品ページを見る
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製品の詳細

What is this antibody validated in?
Anti-Calbindin antibody [EP3478] (ab108404) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF), Multiplex IHC (mIHC) in Human, Mouse, Rat samples.

What is the molecular weight of Calbindin?
Anti-Calbindin [EP3478] (ab108404) specifically detects a band for Calbindin (UniProt: P05937) at a molecular weight of 30kDa.

Trusted by the scientific community
Anti-Calbindin [EP3478] (ab108404) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EP3478] also available for your convenience: ab108404, Alexa Fluor® 647 - ab207528, Alexa Fluor® 488 - ab208377, Carrier free - ab233018, PE - ab303059, APC - ab303060, HRP - ab303061, Alkaline Phosphatase - ab308740, Alexa Fluor® 594 - ab310455, Alexa Fluor® 555 - ab311984, Alexa Fluor® 568 - ab312459, Alexa Fluor® 750 - ab320901

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Stable for 12 months at -20°C

補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Calbindin also known as calbindin-D28k is a calcium-binding protein with a molecular mass of approximately 28 kDa. This target is mainly expressed in the neurons of the brain such as in the cerebellum and hippocampus as well as in the kidneys and intestine. Calbindin plays a mechanical role in binding and buffering intracellular calcium ions which is important for the regulation of calcium-dependent signaling pathways. Researchers often use techniques like Calbindin IHC (immunohistochemistry) and ELISA to study its distribution and presence within tissues.
Biological function summary

Calbindin contributes to cellular homeostasis by managing intracellular calcium levels. It does not appear to be part of a larger protein complex but operates independently. This protein helps to protect neurons from calcium overload potentially safeguarding against excitotoxicity which occurs when neurons die from excessive stimulation. Calbindin's function as a calcium buffer is of particular importance in maintaining neuron function and survival in areas with high neuronal activity.

Pathways

Calbindin has a significant role in the calcium signaling pathway and is also involved in the vitamin D pathway. The calcium signaling pathway is vital for many cellular processes like muscle contraction neurotransmitter release and gene expression. The vitamin D pathway regulates calcium and phosphate homeostasis in the body. Calbindin's relationship with proteins such as calmodulin and parvalbumin both involved in calcium regulation highlights its importance in maintaining cellular functions.

Abnormal Calbindin expression has been observed in conditions like epilepsy and Parkinson's disease. In epilepsy its decreased expression can impact the ability of neurons to regulate calcium properly leading to increased vulnerability to seizures. In Parkinson's disease altered Calbindin levels have been associated with the selective vulnerability of dopamine neurons. These diseases highlight the connection between Calbindin and proteins like alpha-synuclein known for its role in Parkinson's pathology emphasizing the need to understand its contribution to disease mechanisms.

製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

ターゲットの情報

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
See full target information CALB1

文献 (29)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 6:910 PubMed37670183

2023

Activation of TrkB signaling mitigates cerebellar anomalies caused by Rbm4-Bdnf deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Young Tsai,Chiu-Lun Shen,Dhananjaya D,Ching-Yen Tsai,Woan-Yuh Tarn

Cell reports 42:112429 PubMed37141091

2023

Cerebellar granule cell signaling is indispensable for normal motor performance.

Applications

Unspecified application

Species

Unspecified reactive species

Joon-Hyuk Lee,Mehak M Khan,Amanda P Stark,Soobin Seo,Aliya Norton,Zhiyi Yao,Christopher H Chen,Wade G Regehr

eNeuro 10: PubMed37068950

2023

Alcohol Exposure Impairs Retinal Angiogenesis and the Microvessel-Associated Positioning of Calretinin Interneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Marion Dumanoir,Anaïs Leroy,Delphine Burel,Annie Laquerrière,François Janin,Alexis Lebon,Manon Valet,David Godefroy,Lauriane Przegralek,Maryline Lecointre,Serge Picaud,Stéphane Marret,Florent Marguet,Bruno J Gonzalez,Carole Brasse-Lagnel

Nature communications 13:5462 PubMed36115848

2022

Infralimbic medial prefrontal cortex signalling to calbindin 1 positive neurons in posterior basolateral amygdala suppresses anxiety- and depression-like behaviours.

Applications

Unspecified application

Species

Unspecified reactive species

Huiling Yu,Liping Chen,Huiyang Lei,Guilin Pi,Rui Xiong,Tao Jiang,Dongqin Wu,Fei Sun,Yang Gao,Yuanhao Li,Wenju Peng,Bingyu Huang,Guoda Song,Xin Wang,Jingru Lv,Zetao Jin,Dan Ke,Ying Yang,Jian-Zhi Wang

Journal of neurology 270:57-70 PubMed35947153

2022

Homeostatic plasticity of eye movement performance in Xenopus tadpoles following prolonged visual image motion stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Forsthofer,Hans Straka

Nature structural & molecular biology 29:831-840 PubMed35948768

2022

A conformational switch controlling the toxicity of the prion protein.

Applications

Unspecified application

Species

Unspecified reactive species

Karl Frontzek,Marco Bardelli,Assunta Senatore,Anna Henzi,Regina R Reimann,Seden Bedir,Marika Marino,Rohanah Hussain,Simon Jurt,Georg Meisl,Mattia Pedotti,Federica Mazzola,Giuliano Siligardi,Oliver Zerbe,Marco Losa,Tuomas Knowles,Asvin Lakkaraju,Caihong Zhu,Petra Schwarz,Simone Hornemann,Matthew G Holt,Luca Simonelli,Luca Varani,Adriano Aguzzi

Molecular brain 15:71 PubMed35945596

2022

Development of a novel peptide to prevent entry of SARS-CoV-2 into lung and olfactory bulb cells of hACE2 expressing mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Su,Dongxu Zhai,Albert H C Wong,Fang Liu

PLoS genetics 18:e1010138 PubMed35404932

2022

Tumor suppressor PALB2 maintains redox and mitochondrial homeostasis in the brain and cooperates with ATG7/autophagy to suppress neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Yanying Huo,Akshada Sawant,Yongmei Tan,Amar H Mahdi,Tao Li,Hui Ma,Vrushank Bhatt,Run Yan,Jake Coleman,Cheryl F Dreyfus,Jessie Yanxiang Guo,M Maral Mouradian,Eileen White,Bing Xia

Life science alliance 5: PubMed35296518

2022

Spatial proteomics finds CD155 and Endophilin-A1 as mediators of growth and invasion in medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Charles Capdeville,Linda Russo,David Penton,Jessica Migliavacca,Milica Zecevic,Alexandre Gries,Stephan Cf Neuhauss,Michael A Grotzer,Martin Baumgartner

International journal of ophthalmology 15:213-220 PubMed35186679

2022

Frequency cumulative effect of subthreshold energy laser-activated remote phosphors irradiation on visual function in guinea pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Fei Zhang,Xiao-Hong Zhou,Rui-Dan Cao,Jun-Bo Song,Dong-Yu Wei,Fu-Jun Duan,Wei Wang,Zuo-Ming Zhang,Tao Chen
View all publications

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