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Anti-Calbindin 抗体 [CB-955] (ab82812)

Reviews (7)Q&A (6)References (58)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [CB-955] (ab82812)
  • Western blot - Anti-Calbindin antibody [CB-955] (ab82812)

Key features and details

  • Mouse monoclonal [CB-955] to Calbindin
  • Suitable for: IHC-FoFr, WB, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Calbindin antibody [CB-955]
    Calbindin 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [CB-955] to Calbindin

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-FoFr, WB, ICC/IF, IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Cow, Human

  • 免疫原

    Synthetic peptide corresponding to Cow Calbindin.
    Database link: P04467

  • ポジティブ･コントロール

    • IHC-P: Rat cerebellum.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 10th July 2019. Lot numbers higher than GR3258885 are from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab82812の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-FoFr (6)
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 20542987
IHC-P (1)
Use a concentration of 10 - 20 µg/ml.
特記事項
IHC-FoFr
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration. PubMed: 20542987
IHC-P
Use a concentration of 10 - 20 µg/ml.

ターゲット情報

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [CB-955] (ab82812)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [CB-955] (ab82812)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellum tissue labelling Calbindin with ab82812 at 10 µg/mL.

  • Western blot - Anti-Calbindin antibody [CB-955] (ab82812)
    Western blot - Anti-Calbindin antibody [CB-955] (ab82812)
    Lane 1 : Anti-Calbindin antibody [CB-955] (ab82812) at 4 µg/ml
    Lane 2 : No primary control

    All lanes : MDBK cell extract

    Secondary
    All lanes : Goat Anti-Mouse IgG (HRP)

    Developed using the ECL technique.

参考文献 (58)

ab82812 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab82812 は 58 報の論文で使用されています。

  • Wang X  et al. SOX2-positive retinal stem cells are identified in adult human pars plicata by single-cell transcriptomic analyses. MedComm (2020) 4:e198 (2023). PubMed: 36582303
  • Cai L  et al. Dietary sodium enhances the expression of SLC4 family transporters, IRBIT, L-IRBIT, and PP1 in rat kidney: Insights into the molecular mechanism for renal sodium handling. Front Physiol 14:1154694 (2023). PubMed: 37082243
  • Linne C  et al. Encephalopsin (OPN3) is required for normal refractive development and the GO/GROW response to induced myopia. Mol Vis 29:39-57 (2023). PubMed: 37287644
  • Chen X  et al. Transcriptomic mapping uncovers Purkinje neuron plasticity driving learning. Nature 605:722-727 (2022). PubMed: 35545673
  • D'Souza SP  et al. Retinal patterns and the cellular repertoire of neuropsin (Opn5) retinal ganglion cells. J Comp Neurol 530:1247-1262 (2022). PubMed: 34743323
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-10 of 13 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Calbindin antibody [CB-955]

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (BRAIN)
Antigen retrieval step
None
Permeabilization
Yes - TRITON 1%
Specification
BRAIN
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mrs. Francoise Geffroy

Verified customer

投稿 Feb 22 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Calbindin antibody [CB-955]

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
None
Permeabilization
Yes - 1% TirtonX-100
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mrs. Francoise Geffroy

Verified customer

投稿 Feb 19 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Calbindin antibody [CB-955]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Monkey Tissue sections (brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate 10mM Tween 20 0.5%
Permeabilization
Yes - triton 1%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mrs. Francoise Geffroy

Verified customer

投稿 Mar 08 2019

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Calbindin antibody [CB-955]

 Poor
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (macaque retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH 6
Permeabilization
No
Specification
macaque retina
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Nov 09 2018

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Calbindin antibody [CB-955]

 Excellent
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Sodium Citrate pH 8.5 for 30 min at 80°C followed by 30 min cooling to room temperature
Permeabilization
Yes - 0.2% TritonX-100 added to blocking and antibody dilution buffer
Blocking step
10% normal donkey serum + 0.3 M Glycine as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Lars Riecken

Verified customer

投稿 Jun 22 2012

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Calbindin antibody [CB-955]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Brain - Cerebral Cortex)
Specification
Brain - Cerebral Cortex
Fixative
Paraformaldehyde
Permeabilization
No
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Sophie Pezet

Verified customer

投稿 Mar 21 2012

Question

Dear xxxx,
thank you for your prompt and kind reply! The information about the concentration you provided me it will be very useful for replying the Referee

The most important thing is that your Ab worked properly at thesuggesteddilution .

Thank you again for your time and disponibility,

Regards,

Read More
Answer

That's my pleasure. If you do have any further questions please do not hesitate to contact us again.

I wish you all the best with your publication.

Read More

Question

Dear xxxx,
thank you for your kind and exhaustive reply.

I replied the Referee as you suggested (se below); however, it is still not enough! I need to put in the text the concentration of the antibody I used (ab82812). So, I am asking you if you could provide me the starting concentration of the Ab. Maybe I am wrong but in the website ( https://www.abcam.com/Calbindin-antibody-CB-955-ab82812.html) I did not find this detail.

Thank you again for you time and disponibility,

Best regards

Read More
Answer

I am sorry for the delay in getting back to you.

I can now confirm that the concentration of the antibody is 750 ug/mL. This is the case for most lots of this antibody. If you would like me to check the particular lot which you have used I would be pleased to do this for you if you could let me know the lot number.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

Read More

Question

Dear xxxx,
thank you for your kind and exhaustive reply.

I replied the Referee as you suggested (se below); however, it is still not enough! I need to put in the text the concentration of the antibody I used (ab82812). So, I am asking you if you could provide me the starting concentration of the Ab. Maybe I am wrong but in the website ( https://www.abcam.com/Calbindin-antibody-CB-955-ab82812.html) I did not find this detail.

Thank you again for you time and disponibility,

Best regards

Read More
Answer

Thank you for getting back to me and sorry for the delay in my reply.

I am looking into whether the concentration of the anti-Calbindin antibody [CB-955] (ab82812) has been determined. The antibody is provided as that collected from ascites and is therefore a mix of different proteins. The concentration of the antibody portion of this sample may therefore not have been analysed however I will try to obtain this information for you if it is available.

I am sorry forthe delay and any inconvenience this may cause.

Read More

Question

Inquiry: Dear Abcam. I am writing you for having some more information about the antibody ab82812 (anti-calbindin). I used it in rat brain perfused in PFA 4% for IF as a neuronal marker in the the deep cerebellar nuclei. I got good results but a Referee raised the question that it cannot be used as a specific neuronal marker but just a marker for Purkinje cells. I definitely disagree with him! Your datasheet is pretty clear about the use of this ab as neuronal marker and caldibin is commonly used in this way (...). What do you think about? Do you suggest me a possible reply? Thank you for your time and your attention. Kind regards,

Read More
Answer

Thank you for contacting us.

I am glad you have found the anti-Calbindin antibody [CB-955] (ab82812) of use in staining your rat brain tissue.

I, like you, have found several references where Calbindin has been used as a neuronal marker. I include a sample below. I am sorry that the referee of your paper does not support this. I would suggest doing a comprehensivejournal search to find prominent papers where anti-Calbindin antibodies have been used for this purpose and present this to the referee.

I am sorry that I could not be of more help in this instance. I wish you all the best with your publication and your future research.

References whereCalbindin has been used as a neuronal marker:

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0032689

Read More

1-10 of 13 Abreviews or Q&A

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