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AB82812

Anti-Calbindin 抗体 [CB-955]

Anti-Calbindin antibody [CB-955]

4

(8 Reviews)

|

(58 Publications)

Anti-Calbindin antibody [CB-955] (ab82812) is a mouse monoclonal antibody detecting Calbindin in Western Blot, IHC-P. Suitable for Cow, Human, Mouse, Rat.

- Over 50 publications
- Trusted since 2009

別名を表示する

Calbindin, Calbindin D28, D-28K, CALB1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [CB-955] (AB82812)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Calbindin antibody [CB-955] (AB82812)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebellum tissue labelling Calbindin with ab82812 at 10 μg/mL.

Western blot - Anti-Calbindin antibody [CB-955] (AB82812)
  • WB

Supplier Data

Western blot - Anti-Calbindin antibody [CB-955] (AB82812)

Lane 1:

Western blot - Anti-Calbindin antibody [CB-955] (ab82812) at 4 µg/mL

Lane 2:

No primary control

All lanes:

MDBK cell extract

Secondary

All lanes:

Goat Anti-Mouse IgG (HRP)

Predicted band size: 30 kDa

true

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

CB-955

アイソタイプ

IgG1

キャリアフリー

No

交差種

Rat, Cow, Mouse, Human

アプリケーション

IHC-P, WB

applications

免疫原

Synthetic Peptide within Cow CALB1. The exact immunogen used to generate this antibody is proprietary information.

P04467

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Reactivity data

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下記製品にもご興味をお持ちいただけるかもしれません:

AB218270

Human Calbindin ELISA Kit

0

0 Reviews

製品ページを見る
こちらの製品も推奨する理由

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製品の詳細

What is this antibody validated in?
Anti-Calbindin antibody [CB-955] (ab82812) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Cow, Human, Mouse, Rat samples.

Trusted by the scientific community
Anti-Calbindin [CB-955] (ab82812) was first used in a scientific publication in 2009 and has been cited over 50 times in peer-reviewed journals.

Reviewed by scientists
Anti-Calbindin [CB-955] (ab82812) has over 5 independent reviews from customers.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
精製に関する特記事項
Purified from hybridoma cell culture is Protein A column.
バッファー組成
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Calbindin also known as calbindin-D28k is a calcium-binding protein with a molecular mass of approximately 28 kDa. This target is mainly expressed in the neurons of the brain such as in the cerebellum and hippocampus as well as in the kidneys and intestine. Calbindin plays a mechanical role in binding and buffering intracellular calcium ions which is important for the regulation of calcium-dependent signaling pathways. Researchers often use techniques like Calbindin IHC (immunohistochemistry) and ELISA to study its distribution and presence within tissues.
Biological function summary

Calbindin contributes to cellular homeostasis by managing intracellular calcium levels. It does not appear to be part of a larger protein complex but operates independently. This protein helps to protect neurons from calcium overload potentially safeguarding against excitotoxicity which occurs when neurons die from excessive stimulation. Calbindin's function as a calcium buffer is of particular importance in maintaining neuron function and survival in areas with high neuronal activity.

Pathways

Calbindin has a significant role in the calcium signaling pathway and is also involved in the vitamin D pathway. The calcium signaling pathway is vital for many cellular processes like muscle contraction neurotransmitter release and gene expression. The vitamin D pathway regulates calcium and phosphate homeostasis in the body. Calbindin's relationship with proteins such as calmodulin and parvalbumin both involved in calcium regulation highlights its importance in maintaining cellular functions.

Abnormal Calbindin expression has been observed in conditions like epilepsy and Parkinson's disease. In epilepsy its decreased expression can impact the ability of neurons to regulate calcium properly leading to increased vulnerability to seizures. In Parkinson's disease altered Calbindin levels have been associated with the selective vulnerability of dopamine neurons. These diseases highlight the connection between Calbindin and proteins like alpha-synuclein known for its role in Parkinson's pathology emphasizing the need to understand its contribution to disease mechanisms.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Buffers cytosolic calcium. May stimulate a membrane Ca(2+)-ATPase and a 3',5'-cyclic nucleotide phosphodiesterase.
See full target information CALB1
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文献 (58)

Recent publications for all applications. Explore the full list and refine your search

Molecular vision 29:39-57 PubMed37287644

2023

Encephalopsin (OPN3) is required for normal refractive development and the GO/GROW response to induced myopia.

Applications

Unspecified application

Species

Unspecified reactive species

Courtney Linne,Khine Yin Mon,Shane D'Souza,Heonuk Jeong,Xiaoyan Jiang,Dillon M Brown,Kevin Zhang,Shruti Vemaraju,Kazuo Tsubota,Toshihide Kurihara,Machelle T Pardue,Richard A Lang

Frontiers in physiology 14:1154694 PubMed37082243

2023

Dietary sodium enhances the expression of SLC4 family transporters, IRBIT, L-IRBIT, and PP1 in rat kidney: Insights into the molecular mechanism for renal sodium handling.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Cai,Dengke Wang,Tianxiang Gui,Xiaoyu Wang,Lingyu Zhao,Walter F Boron,Li-Ming Chen,Ying Liu

MedComm 4:e198 PubMed36582303

2022

SOX2-positive retinal stem cells are identified in adult human pars plicata by single-cell transcriptomic analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaotang Wang,Wei Fan,Zongren Xu,Qi Zhang,Na Li,Ruonan Li,Guoqing Wang,Siyuan He,Wanqian Li,Dan Liao,Zhi Zhang,Nan Shu,Jiaxing Huang,Chenyang Zhao,Shengping Hou

Neuron 110:3278-3287.e8 PubMed36070749

2022

LINE-1 activation in the cerebellum drives ataxia.

Applications

Unspecified application

Species

Unspecified reactive species

Takehiro Takahashi,Milan Stoiljkovic,Eric Song,Xiao-Bing Gao,Yuki Yasumoto,Eriko Kudo,Fernando Carvalho,Yong Kong,Annsea Park,Marya Shanabrough,Klara Szigeti-Buck,Zhong-Wu Liu,Ashley Kristant,Yalan Zhang,Parker Sulkowski,Peter M Glazer,Leonard K Kaczmarek,Tamas L Horvath,Akiko Iwasaki

Frontiers in cell and developmental biology 10:931326 PubMed35874803

2022

KCC3a, a Strong Candidate Pathway for K Loss in Alkalemia.

Applications

Unspecified application

Species

Unspecified reactive species

Mohammed Zubaerul Ferdaus,Andrew Scott Terker,Rainelli Koumangoye,Eric Delpire

Nature 605:722-727 PubMed35545673

2022

Transcriptomic mapping uncovers Purkinje neuron plasticity driving learning.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying Chen,Yanhua Du,Gerard Joey Broussard,Mikhail Kislin,Carla M Yuede,Shuwei Zhang,Sabine Dietmann,Harrison Gabel,Guoyan Zhao,Samuel S-H Wang,Xiaoqing Zhang,Azad Bonni

Frontiers in molecular neuroscience 15:822129 PubMed35557557

2022

Social Deficits and Cerebellar Degeneration in Purkinje Cell Knockout Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofan Yang,Hongqiang Yin,Xiaojing Wang,Yueqing Sun,Xianli Bian,Gaorui Zhang,Anning Li,Aihua Cao,Baomin Li,Darius Ebrahimi-Fakhari,Zhuo Yang,Miriam H Meisler,Qiji Liu

FEBS letters 596:294-308 PubMed34890048

2021

The chromatin repressors EZH2 and Suv4-20h coregulate cell fate specification during hippocampal development.

Applications

Unspecified application

Species

Unspecified reactive species

Kai-Chun Chang,Christopher T Rhodes,Jesse Q Zhang,Madeleine C Moseley,Sandra M Cardona,Shu-Wei Angela Huang,Ashley Rawls,Vance P Lemmon,Mitchel S Berger,Adam R Abate,Chin-Hsing Annie Lin

Scientific reports 11:23580 PubMed34880332

2021

Post-translational modifications by SIRT3 de-2-hydroxyisobutyrylase activity regulate glycolysis and enable nephrogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Perico,Marina Morigi,Anna Pezzotta,Daniela Corna,Valerio Brizi,Sara Conti,Cristina Zanchi,Fabio Sangalli,Piera Trionfini,Sara Buttò,Christodoulos Xinaris,Susanna Tomasoni,Carlamaria Zoja,Giuseppe Remuzzi,Ariela Benigni,Barbara Imberti

The Journal of comparative neurology 530:1247-1262 PubMed34743323

2021

Retinal patterns and the cellular repertoire of neuropsin (Opn5) retinal ganglion cells.

Applications

Unspecified application

Species

Unspecified reactive species

Shane P D'Souza,David I Swygart,Sophia R Wienbar,Brian A Upton,Kevin X Zhang,Robert D Mackin,Anna K Casasent,Melanie A Samuel,Gregory W Schwartz,Richard A Lang
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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