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AB325662

Anti-CALB2 antibody [L122/6] - Chicken IgY (Chimeric)

Anti-CALB2 antibody [L122/6] - Chicken IgY (Chimeric)

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Chicken Monoclonal Calretinin antibody. Suitable for IHC-P, WB and reacts with Rat samples.

別名を表示する

Calretinin, CR

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALB2 antibody [L122/6] - Chicken IgY (Chimeric) (AB325662)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALB2 antibody [L122/6] - Chicken IgY (Chimeric) (AB325662)

Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing cells stained by ab325662 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. Magnification 100X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALB2 antibody [L122/6] - Chicken IgY (Chimeric) (AB325662)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CALB2 antibody [L122/6] - Chicken IgY (Chimeric) (AB325662)

Representative section of formalin fixed, paraffin-embedded rat brain cerebral cortex showing staining cells by ab325662 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. Magnification 400X.

Western blot - Anti-CALB2 antibody [L122/6] - Chicken IgY (Chimeric) (AB325662)
  • WB

Supplier Data

Western blot - Anti-CALB2 antibody [L122/6] - Chicken IgY (Chimeric) (AB325662)

Western blotting of rat whole brain homogenate with an anti-Calretinin mouse mAb and ab325662 antibodies (10 µg/ml). The mouse mAb and ab325662 were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. The antibody recognizes endogenous CALB2 with similar sensitivity as the anti-Calretinin mouse Mab in the rat whole brain homogenate at 30 kDa.

Lane 1:

anti-Calretinin mouse mAb at 10 µg/mL

Lane 2:

Western blot - Anti-CALB2 antibody [L122/6] - Chicken IgY (Chimeric) (ab325662) at 10 µg/mL

All lanes:

rat whole brain homogenate

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 30 kDa

false

Key facts

宿主種

Chicken

クローン性

Monoclonal

クローン番号

L122/6

アイソタイプ

IgY

キャリアフリー

No

交差種

Rat

アプリケーション

IHC-P, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特異性

No cross-reactivity reported.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "<p></p>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification
精製に関する特記事項
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L122/6 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L122/6 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
バッファー組成
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Calcium-binding protein involved in calcium homeostasis and signal transduction. It plays a critical role in buffering intracellular calcium levels and modulating calcium-dependent signaling pathways. Predominantly expressed in specific neuronal populations, influences synaptic plasticity and neuronal excitability, contributing to learning and memory (By similarity). During embryonic development, it facilitates neuronal differentiation and maturation (PubMed : 14664811).
See full target information Calb2
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Abcam product promise

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