AB270294

Anti-Cadherin 16 抗体 [CDH16/1532R] - BSA and Azide free

Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free

Recombinant
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Rabbit Recombinant Monoclonal Cadherin 16 antibody. Carrier free. Suitable for Flow Cyt, WB, IHC-P and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDH16.

別名を表示する

UNQ695/PRO1340, CDH16, Cadherin-16, Kidney-specific cadherin, Ksp-cadherin

4 Images

Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Flow cytometric analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells labeling Cadherin 16 (Blue) using ab270263 at 2μg/10⁶ cells followed by goat anti-rabbit IgG-CF488; isotype control (Red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Formalin-fixed, paraffin-embedded human renal cell carcinoma stained for Cadherin 16 using ab270263 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Formalin-fixed, paraffin-embedded rat kidney tissue stained for Cadherin 16 using ab270263 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Formalin-fixed, paraffin-embedded mouse kidney tissue stained for Cadherin 16 using ab270263 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab270263).

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

CDH16/1532R

アイソタイプ

IgG

キャリアフリー

Yes

交差種

Mouse, Rat, Human

アプリケーション

Flow Cyt, IHC-P, WB

applications

免疫原

Recombinant Full Length Protein corresponding to Human CDH16.

O75309

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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製品の詳細

ab270294 is a carrier free version of ab270263.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein A

バッファー組成

pH: 7.2 - 7.4 Constituents: PBS

出荷温度

Blue Ice

短期保存期間

1-2 weeks

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Cadherin 16 also known as Ksp-cadherin is a calcium-dependent cell adhesion protein. It plays a role in mediating homophilic cell-cell adhesion facilitating the binding between identical cadherins on neighboring cells. The mass of cadherin 16 is approximately 92 kDa. This protein is mainly expressed in the kidney specifically in the distal convoluted tubules and collecting ducts where it is localized to the basolateral membrane of tubular epithelial cells.

Biological function summary

Cadherin 16 contributes to cell sorting and tissue morphogenesis. It does not operate as part of a multi-protein complex but interacts with the actin cytoskeleton through catenins like β-catenin to maintain cell adhesion and tissue integrity. The particular expression pattern of cadherin 16 suggests that it has a role in maintaining the structural organization of renal tissues and possibly influences renal tubular epithelial homeostasis.

Pathways

Cadherin 16 participates in cell signaling pathways essential for cell adhesion and tissue architecture. Specifically it engages in the Wnt signaling pathway where it coordinates with proteins like β-catenin indicating its indirect role in signal transduction that may affect gene transcription regarding cell growth and differentiation. Another key interaction for cadherin 16 is with integrins which are pivotal in mediating attachments between the extracellular matrix and the cell.

Cadherin 16 is linked to renal-related conditions such as renal cell carcinoma and polycystic kidney disease. Abnormal expression or dysfunction of cadherin 16 could disrupt cell adhesion promoting renal cell carcinoma by facilitating cell detachment and metastatic progression. Additionally it connects with proteins like E-cadherin which are involved in epithelial-to-mesenchymal transition (EMT) a process often activated in cancer development and progression.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types.

See full target information CDH16

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cadherin 16 抗体 [CDH16/1532R] - BSA and Azide free

Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free

Flow Cytometry - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cadherin 16 antibody [CDH16/1532R] - BSA and Azide free (AB270294)

関連する標識済み抗体及び組成の異なる製品 (1)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

組合せ製品

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

バッファー組成

出荷温度

短期保存期間

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

Abcam product promise