Anti-CacyBP 抗体 [EPR12374] (ab171972)
![Western blot - Anti-CacyBP antibody [EPR12374] (ab171972)](https://www.abcam.co.jp/ps/products/171/ab171972/Images/ab171972-194476-anti-siah-interacting-protein-antibody-epr12374-western-blot.jpg "Western blot - Anti-CacyBP antibody [EPR12374] (ab171972)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12374] to CacyBP
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CacyBP antibody [EPR12374]
CacyBP 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR12374] to CacyBP -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IF, IPmore details -
種交差性
交差種: Human
非交差種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ･コントロール
- Human kidney, gastric carcinoma and gliocytoma tissues; MCF7, HeLa, 293T and PC3 cell lysates; MCF7 and 293T cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR12374 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab171972の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| WB |
1/10000 - 1/50000. Predicted molecular weight: 26 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/250.
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| IP |
1/10 - 1/100.
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| 特記事項 |
|---|
|
Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB
1/10000 - 1/50000. Predicted molecular weight: 26 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/250. |
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IP
1/10 - 1/100. |
ターゲット情報
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機能
May be involved in calcium-dependent ubiquitination and subsequent proteasomal degradation of target proteins. Probably serves as a molecular bridge in ubiquitin E3 complexes. Participates in the ubiquitin-mediated degradation of beta-catenin (CTNNB1). -
配列類似性
Contains 1 CS domain.
Contains 1 SGS domain. -
翻訳後修飾
Phosphorylated on serine residues. Phosphorylated upon induction by RA or at high calcium concentrations. -
細胞内局在
Nucleus. Cytoplasm. Cytoplasmic at low calcium concentrations. In neuroblastoma cells, after a retinoic acid (RA) induction and calcium increase, it localizes in both the nucleus and cytoplasm. The nuclear fraction may be phosphorylated. - Information by UniProt
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参照データベース
- Entrez Gene: 27101 Human
- Omim: 606186 Human
- SwissProt: Q9HB71 Human
- Unigene: 508524 Human
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別名
- CacyBP antibody
- Calcyclin binding protein antibody
- Calcyclin-binding protein antibody
see all
画像
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Western blot - Anti-CacyBP antibody [EPR12374] (ab171972)All lanes : Anti-CacyBP antibody [EPR12374] (ab171972) at 1/10000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : 293T cell lysate
Lane 4 : PC3 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 26 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)](https://www.abcam.co.jp/ps/products/171/ab171972/Images/ab171972-194474-anti-siah-interacting-protein-antibody-epr12374-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)Immunohistochemical analysis of paraffin-embedded Human Kidney tissue labeling CacyBP with ab171972 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)](https://www.abcam.co.jp/ps/products/171/ab171972/Images/ab171972-194473-anti-siah-interacting-protein-antibody-epr12374-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling CacyBP with ab171972 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)](https://www.abcam.co.jp/ps/products/171/ab171972/Images/ab171972-194472-anti-siah-interacting-protein-antibody-epr12374-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CacyBP antibody [EPR12374] (ab171972)Immunohistochemical analysis of paraffin-embedded Human gastric carcinoma tissue labeling CacyBP with ab171972 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-CacyBP antibody [EPR12374] (ab171972)](https://www.abcam.co.jp/ps/products/171/ab171972/Images/ab171972-194471-anti-siah-interacting-protein-antibody-epr12374-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CacyBP antibody [EPR12374] (ab171972)Immunofluorescent analysis of MCF7 cells labeling CacyBP with ab171972 at 1/100 dilution. DAPI nuclear staining (blue).
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![Immunoprecipitation - Anti-CacyBP antibody [EPR12374] (ab171972)](https://www.abcam.co.jp/ps/products/171/ab171972/Images/ab171972-194475-anti-siah-interacting-protein-antibody-epr12374-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CacyBP antibody [EPR12374] (ab171972)Western blot analysis on immunoprecipitation pellet from 293T cell lysate (lane 1) labeling CacyBP with ab171972 at 1/10 dilution or 1X PBS (lane 2) negative control. HRP-conjugated anti-Rabbit IgG preferentially detecting the non-reduced form of Rabbit IgG.
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![Flow Cytometry (Intracellular) - Anti-CacyBP antibody [EPR12374] (ab171972)](https://www.abcam.co.jp/ps/products/171/ab171972/Images/ab171972-194469-anti-siah-interacting-protein-antibody-epr12374-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CacyBP antibody [EPR12374] (ab171972)Intracellular flow cytometric analysis of permeabilized 293T cells labeling CacyBPwith ab171972 at 1/10 dilution (red) orRabbit IgG (negative) (green).
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![Anti-CacyBP antibody [EPR12374] (ab171972)](https://www.abcam.co.jp/ps/products/171/ab171972/Images/ab171972-8-benefits-of-recombinant-antibodies.png)
Anti-CacyBP antibody [EPR12374] (ab171972)
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab171972 は論文での使用が確認できていません。