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AB134269

Anti-CACNA1G 抗体 [N178A/9]

Anti-CACNA1G antibody [N178A/9]

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(4 Publications)

Mouse Monoclonal Voltage-dependent T-type calcium channel subunit alpha-1G antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 4 publications.
3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CACNA1G antibody [N178A/9] (AB134269)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CACNA1G antibody [N178A/9] (AB134269)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling CACNA1G with ab134269 at 1/50 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav3.1 Antibody (D) Composite.

Western blot - Anti-CACNA1G antibody [N178A/9] (AB134269)
  • WB

Unknown

Western blot - Anti-CACNA1G antibody [N178A/9] (AB134269)

All lanes:

Western blot - Anti-CACNA1G antibody [N178A/9] (ab134269) at 1/1000 dilution

All lanes:

Rat brain membrane tissue lysate

Secondary

All lanes:

Goat anti-mouse IgG HRP

Predicted band size: 262 kDa

true

Immunocytochemistry/ Immunofluorescence - Anti-CACNA1G antibody [N178A/9] (AB134269)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CACNA1G antibody [N178A/9] (AB134269)

4% formaldehyde-fixed SK-N-BE cells stained for CACNA1G (green) using ab134269 at 1/100 dilution in ICC/IF.

Secondary Antibody : Goat Anti-Mouse ATTO 488 at 1/200 for 60 min at room temperature. Counterstain : Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000; 1/5000 for 60 min room temperature, 5 min room temperature.

A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ab134269. (D) Composite.

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

N178A/9

アイソタイプ

IgG1

キャリアフリー

No

交差種

Rat, Human

アプリケーション

WB, ICC/IF

applications

特異性

ab134269 does not cross-react with CACNA1H (Cav3.2).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>1μg/ml of ab134269 is sufficient for detection of CACNA1G in 20μg of Rat brain membrane lysate.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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製品の詳細

The clone number has been updated from S178A-9 to N178A/9, both clone numbers name the same antibody clone.
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
Preservative: 0.09% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The protein CACNA1G also known as Cav3.1 is a T-type voltage-gated calcium channel. This channel has a mass of approximately 251 kDa. CACNA1G primarily functions in the passage of calcium ions across the cell membrane. It is expressed in various tissues with high levels in the brain heart and kidney. Its presence in neurons and cardiac cells contributes to the low-voltage activated calcium currents important for cellular excitability.
Biological function summary

CACNA1G conducts calcium currents and influences cellular activities like neuronal firing and pacemaker potentials. CACNA1G forms part of a larger complex involving other voltage-gated calcium channels which work together to regulate calcium signaling. It modulates processes such as neurotransmitter release muscle contraction and gene expression. By controlling calcium ion influx it plays a role in maintaining intracellular calcium levels important for cellular function.

Pathways

CACNA1G is involved in calcium signaling and cardiac action potential pathways. These pathways are important for the regulation of heart rhythm and neuronal activities. CACNA1G interacts with proteins such as calmodulin influencing calcium channel function and subsequent signaling events. It also connects to the broader pathway of excitation-contraction coupling in cardiac tissues and neurotransmission in neurons.

CACNA1G has been linked to epilepsy and heart arrhythmias. Mutations in CACNA1G can alter calcium channel function affecting neuronal excitability and potentially leading to epileptic seizures. In cardiac tissues abnormalities in CACNA1G function can disrupt the cardiac action potential contributing to arrhythmias. Researchers have noted connections between CACNA1G and other ion channel proteins influencing our understanding of these disease mechanisms.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

See full target information Voltage-dependent T-type calcium channel subunit alpha-1G
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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Environmental toxicology 39:357-366 PubMed37755144

2023

MiRNA-26a-5p inhibits preterm labor initiation by targeting and regulating TRPC3 ion channel protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Chen,Tong Liu,Hong Cui,Quan Na,Sishi Liu

Translational oncology 14:101152 PubMed34134073

2021

PTPN3 is a potential target for a new cancer immunotherapy that has a dual effect of T cell activation and direct cancer inhibition in lung neuroendocrine tumor.

Applications

Unspecified application

Species

Unspecified reactive species

Satoko Koga,Hideya Onishi,Shogo Masuda,Akiko Fujimura,Shu Ichimiya,Kazunori Nakayama,Akira Imaizumi,Kenichi Nishiyama,Masayuki Kojima,Kei Miyoshi,Katsuya Nakamura,Masayo Umebayashi,Takashi Morisaki,Masafumi Nakamura

Biomolecules & therapeutics 28:389-396 PubMed32319264

2020

Epigenetically Upregulated T-Type Calcium Channels Contribute to Abnormal Proliferation of Embryonic Neural Progenitor Cells Exposed to Valproic Acid.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Woon Kim,Hyun Ah Oh,Sung Rae Kim,Mee Jung Ko,Hana Seung,Sung Hoon Lee,Chan Young Shin

Biomolecules & therapeutics 26:439-445 PubMed29463073

2018

T-Type Calcium Channels Are Required to Maintain Viability of Neural Progenitor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ji-Woon Kim,Hyun Ah Oh,Sung Hoon Lee,Ki Chan Kim,Pyung Hwa Eun,Mee Jung Ko,Edson Luck T Gonzales,Hana Seung,Seonmin Kim,Geon Ho Bahn,Chan Young Shin
View all publications
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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。
保証に関する詳細については利用規約をご確認ください。
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