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Anti-CACNA1C 抗体 (ab81980)

Submit a review Q&A (6)References (3)
Western blot - Anti-CACNA1C antibody (ab81980)

    Key features and details

    • Goat polyclonal to CACNA1C
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-CACNA1C antibody
      CACNA1C 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to CACNA1C

    • 由来種

      Goat

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Cynomolgus monkey

    • 免疫原

      Synthetic peptide corresponding to Human CACNA1C aa 2201-2213 (C terminal).
      Sequence:

      C-RARGRPSEEELQD

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • Human Heart and Skeletal Muscle lysates.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab81980の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 249 kDa (predicted molecular weight: 249 kDa).

    1 hour primary incubation is recommended for this product.
    特記事項
    WB
    Use a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 249 kDa (predicted molecular weight: 249 kDa).

    1 hour primary incubation is recommended for this product.

    ターゲット情報

    • 機能

      Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. The isoform alpha-1C gives rise to L-type calcium currents. Long-lasting (L-type) calcium channels belong to the 'high-voltage activated' (HVA) group. They are blocked by dihydropyridines (DHP), phenylalkylamines, benzothiazepines, and by omega-agatoxin-IIIA (omega-Aga-IIIA). They are however insensitive to omega-conotoxin-GVIA (omega-CTx-GVIA) and omega-agatoxin-IVA (omega-Aga-IVA). Calcium channels containing the alpha-1C subunit play an important role in excitation-contraction coupling in the heart. The various isoforms display marked differences in the sensitivity to DHP compounds. Binding of calmodulin or CABP1 at the same regulatory sites results in an opposit effects on the channel function.

    • 組織特異性

      Expressed in brain, heart, jejunum, ovary, pancreatic beta-cells and vascular smooth muscle. Overall expression is reduced in atherosclerotic vascular smooth muscle.

    • 関連疾患

      Timothy syndrome
      Brugada syndrome 3

    • 配列類似性

      Belongs to the calcium channel alpha-1 subunit (TC 1.A.1.11) family. CACNA1C subfamily.

    • ドメイン

      Each of the four internal repeats contains five hydrophobic transmembrane segments (S1, S2, S3, S5, S6) and one positively charged transmembrane segment (S4). S4 segments probably represent the voltage-sensor and are characterized by a series of positively charged amino acids at every third position.
      Binding of intracellular calcium through the EF-hand motif inhibits the opening of the channel.

    • 翻訳後修飾

      Phosphorylation by PKA activates the channel.

    • 細胞内局在

      Membrane. Cell membrane. The interaction between RRAD and CACNB2 regulates its trafficking to the cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • alpha-1 polypeptide antibody
        • cardiac muscle antibody
        • isoform 1 antibody
        • L type antibody
        • CAC1C_HUMAN antibody
        • CACH 2 antibody
        • CACH2 antibody
        • CACN 2 antibody
        • CACN2 antibody
        • CACNA1C antibody
        • CACNL1A1 antibody
        • Calcium channel antibody
        • Calcium channel cardic dihydropyridine sensitive alpha 1 subunit antibody
        • Calcium channel L type alpha 1 polypeptide isoform 1 cardiac muscle antibody
        • Calcium channel voltage dependent L type alpha 1C subunit antibody
        • CaV1.2 antibody
        • CCHL1A1 antibody
        • DHPR alpha 1 antibody
        • DHPR alpha 1 subunit antibody
        • LQT8 antibody
        • TS antibody
        • Voltage dependent L type calcium channel alpha 1C subunit antibody
        • Voltage dependent L type calcium channel subunit alpha 1C antibody
        • Voltage gated calcium channel alpha subunit Cav1.2 antibody
        • Voltage gated calcium channel subunit alpha Cav1.2 antibody
        • Voltage gated L type calcium channel Cav1.2 alpha 1 subunit, splice variant 10* antibody
        • Voltage-dependent L-type calcium channel subunit alpha-1C antibody
        • Voltage-gated calcium channel subunit alpha Cav1.2 antibody
        see all

      画像

      • Western blot - Anti-CACNA1C antibody (ab81980)
        Western blot - Anti-CACNA1C antibody (ab81980)
        Anti-CACNA1C antibody (ab81980) at 0.5 µg/ml + Human Heart lysate in RIPA buffer at 35 µg

        Predicted band size: 249 kDa
        Observed band size: 249 kDa



        Primary incubation was 1 hour. Detected by chemiluminescence.

      参考文献 (3)

      ab81980 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab81980 は 3 報の論文で使用されています。

      • Papp R  et al. Genomic upregulation of cardiac Cav1.2a and NCX1 by estrogen in women. Biol Sex Differ 8:26 (2017). PubMed: 28807015
      • Papp R  et al. Genomic upregulation of cardiac Cav1.2a and NCX1 by estrogen in women. Biol Sex Differ 8:26 (2017). PubMed: 28815011
      • Herraiz-Martínez A  et al. Ageing is associated with deterioration of calcium homeostasis in isolated human right atrial myocytes. Cardiovasc Res 106:76-86 (2015). PubMed: 25712961

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-6 of 6 Abreviews or Q&A

      Question

      Thank you for your former advice and replacement antibody for DHPR alpha 1 (aka CaV 1.2 alpha 1).

      Now we have tried Ab 81980 with our human heart samples, but unfortunately it does not seem to work either, as it gives some aspecific band with a smaller MW than expected and no band occurs at the expected MW range.

      I attach a ppt slide with the result we have got.

      In the meanwhile, we have tried another antibody and that one works fine, the band coming at the right MW and control peptide blocking showed that it was a specific band. We are going to use that antibody for our further studies.

      I would like to ask if we can still get a refund or credit which we can spend purhasing some other (not CaV 1.2) antibody?

      Read More
      Answer

      Thank you for your reply and for completing of customer survey follow up email.
      I am sorry that the replacement antibody that we sent you, ab81980 did not prove to be successful for you.
      Therefore I have processed your request to receive a refund for the original antibody, ab81814.
      Please let me know if there is anything else I can help you with.

      Read More

      Question

      Thank you for your advice, after looking at the data sheets one more time, I also think that ab81980 has the better chances, and I was impressed by the blot presented on the data sheet.

      So we would like to try ab81980, would you be so kind to sent that one?

      Thank you,

      Read More
      Answer

      Thank you for contacting Abcam.
      I have processed your request to receive ab81980 as a replacement for ab84814 and your new order number is ********.
      I hope that this new antibody is successful and if there is anything else I can help you with, please let me know.

      Read More

      Question

      Thank you for your help. The ordering information is correct, that is us.

      I will have one more look at those antibody fact sheets today and decide which one to try - do you happen to know if any of them has good references for western blot, preferably on human samples?

      Read More
      Answer

      Thank you for reply.
      Out of either ab81980 or ab8109, I would recommend ab81980, as we have a western blot image for that one on our website. We do not have any more data for either antibody, although both are guaranteed to work in western blot on human samples.
      I look forward to your reply and helping you resolve this issue.

      Read More

      Question

      Thank you for your suggestions in order to make ab84814 work better. We have tried the suggestions, however, we still have a very strong background, no specific signal and lots of aspecific bands.

      I attach a word file which details the protocol which we routinely use and which is working with all the other antibodies, and also include the modifications you suggested last time.
      I also inserted a blot image we have got when following your advice.
      I would like to ask whether you have any more suggestions about what and how to modify in our protocol, or we would be happy to try another anti-DHPR antibody, like ab81980 or ab81095.

      Read More
      Answer

      Thank you for your reply.
      I am sorry that the protocol tips did not prove to be successful. Under our Abpromise we do guarantee this antibody for 6 months to work in western blot on human samples. Since we have not been able to resolve this issue for you, then I would be happy to send you a replacement antibody as requested..
      To do that, I need to have your original order information. I believe I have found that information, could you confirm that the product was ordered on 9th Nov 2011 by Bethann Gabris. If this information is incorrect, could you send me the correct information? Also, I can send you either ab81980 or ab81095, just let me know which one you would like to receive.
      I look forward to your reply and resolving this issue for you.

      Read More

      Question

      to the mail below, we ordered and tried "ab 84814" to perform western blot on human heart samples. We are rather disappointed as the antibody does give the specific band around 200 kDa, but it also gives some very heterogeneous background which makes it impossible to get clear image of the bands and analyse band intensity.    I attach a file to show the background I mean, so far we tried it 4 times with 3 different dilutions. Out of the 4, one blot was somewhat usable, the other 3 were unusable. I gave some information on our western blot protocol, if you need more details, please ask and I will write more. However, we are working with several antibodies and we never get this heterogeneous background with any of the others, so we think the problem is not with the protocol but with the antibody.   Could you please tell us what to do differently, or can we try either another batch of this, or a different CaV 1.2 alpha (=DHPR alpha 1) antibody?  

      Read More
      Answer

      Thank you for contacting Abcam. I am sorry that you are having problems with ab84814 in western blot. I have looked over the images and protocol information that you sent and there are a few protocol changes that I would suggest that may help to reduce the background that you are seeing: 1 - Switch to a 5% BSA block rather than 5% Milk, as BSA is a little better a blocking non-specific binding. 2 - Increase the number of wash steps you are doing (up 5 washes, 5 minutes each) and add 0.05% Tween 20 to the wash buffer, if you have not done so already. 3 - After the secondary incubation, wash the membrane for 30 minutes in a 0.5M NaCl. Then rinse the membrane thoroughly in dH20 and develop as normal. If these protocol tips do not work then I would be happy to send you a replacement antibody, as under our Abpromise we do guarantee this antibody to work in western blot on human samples. I look forward to your reply and helping you resolve these issues.

      Read More

      Question

      We are looking for a CaV 1.2 (aka DHPR alpha 1, aka CACNA1C) antibody suitable for western blotting on human heart total protein samples.    ab81980 seems the most suitable and the blot provided has no aspecific bands, however, ab84814 has more references and also predicted to react with human samples. Could you tell us which one has the better chance to react with CaV 1.2 without giving aspecific bands?   I also would like to ask about your guarantee policy, is that valid for any of these antibodies? Does it mean we can get our money refunded if the antibody does not perform well, or it means we can order some other antibody?  

      Read More
      Answer

      Thank you for contacting Abcam. Under our Abpromise we guarantee all of our antibodies to work as stated on the datasheet, if they do work as stated and we cannot offer any assistance to resolve the matter, then we would either replace the antibody or give a refund. Both ab81980 and ab84814 are guaranteed to work in western blot on human samples. I would recommended ab84814, even though the western blot image may not be the cleanest. If there is anything else I can help you with, please let me know.

      Read More

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