Anti-CA150 抗体 (ab10481)
Key features and details
- Rabbit polyclonal to CA150
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CA150 antibody
CA150 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CA150 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chimpanzee, Gorilla
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免疫原
Synthetic peptide corresponding to Human CA150 aa 1-100.
Database link: 10915 -
ポジティブ･コントロール
- WB: HeLa and HEK293T cells. IHC-P: human breast carcinoma, mouse squamous cell carcinoma
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using the peptide immobilized on solid support. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab10481の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa).
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| IHC-P |
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 150 kDa). |
|
IHC-P
1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Transcription factor that binds RNA polymerase II and inhibits the elongation of transcripts from target promoters. Regulates transcription elongation in a TATA box-dependent manner. Necessary for TAT-dependent activation of the human immunodeficiency virus type 1 (HIV-1) promoter. -
組織特異性
Detected in brain neurons. -
配列類似性
Contains 6 FF domains.
Contains 3 WW domains. -
ドメイン
The FF domains bind the phosphorylated C-terminus of the largest subunit of RNA polymerase II, probably mediate interaction with HTATSF1 and preferentially bind peptides with the consensus sequence [DE](2-5)-[FWY]-[DE](2-5).
The WW domains bind Pro-rich domains. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10915 Human
- Entrez Gene: 56070 Mouse
- Omim: 605409 Human
- SwissProt: O14776 Human
- SwissProt: Q8CGF7 Mouse
- Unigene: 723884 Human
- Unigene: 270511 Mouse
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別名
- CA 150 antibody
- Co activator of 150 kDa antibody
- Coactivator of 150 kDa antibody
see all
画像
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Western blot - Anti-CA150 antibody (ab10481)All lanes : Anti-CA150 antibody (ab10481) at 1/10000 dilution
Lane 1 : HeLa Whole cell lysate
Lane 2 : HEK293T Whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 150 kDaDetection: Chemiluminescence with an exposure time of 10 seconds.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CA150 antibody (ab10481)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling CA150 at 1/1000 (1µg/ml). Detection: DAB.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab10481 は論文での使用が確認できていません。