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リコンビナントRabMAb

Anti-C7orf42 抗体 [EPR15381] (ab185948)

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Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948)
  • Flow Cytometry (Intracellular) - Anti-C7orf42 antibody [EPR15381] (ab185948)
  • Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948)
  • Anti-C7orf42 antibody [EPR15381] (ab185948)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15381] to C7orf42
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-C7orf42 antibody [EPR15381]
    C7orf42 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR15381] to C7orf42

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Hela, MCF-7, A549, HepG2, C6, Raw264.7, PC-12 and NIH/3T3 cell lysates. Hela cells.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR15381

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185948の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/210.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.
特記事項
Flow Cyt (Intra)
1/210.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
WB
1/1000 - 1/10000. Predicted molecular weight: 35 kDa.

ターゲット情報

画像

  • Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948)
    Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948)
    All lanes : Anti-C7orf42 antibody [EPR15381] (ab185948) at 1/10000 dilution

    Lane 1 : Hela cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : A549 cell lysate
    Lane 4 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa



    Blocking buffer: 5% NFDM/TBST

  • Flow Cytometry (Intracellular) - Anti-C7orf42 antibody [EPR15381] (ab185948)
    Flow Cytometry (Intracellular) - Anti-C7orf42 antibody [EPR15381] (ab185948)

    Intracellular Flow Cytometry analysis of HeLa cells usingab185948 at 1/210 dilution. Secondary ab: Goat anti rabbit IgG (FITC) at 1/150 dilution. Fixative: 2% paraformaldehyde. Isotype control: Rabbit monoclonal IgG. 

     

     

  • Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948)
    Western blot - Anti-C7orf42 antibody [EPR15381] (ab185948)
    All lanes : Anti-C7orf42 antibody [EPR15381] (ab185948) at 1/1000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : Raw264.7 cell lysate
    Lane 3 : PC-12 cell lysate
    Lane 4 : NIH/3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 35 kDa



    Blocking buffer: 5% NFDM/TBST

  • Anti-C7orf42 antibody [EPR15381] (ab185948)
    Anti-C7orf42 antibody [EPR15381] (ab185948)

参考文献 (0)

ab185948 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab185948 は論文での使用が確認できていません。

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