Anti-C5b-9 抗体 (ab55811)
Key features and details
- Rabbit polyclonal to C5b-9
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-C5b-9 antibody -
製品の詳細
Rabbit polyclonal to C5b-9 -
由来種
Rabbit -
特異性
This antibody is monospecific for C5b-9 complex in purified form or present in cobra venom factor activated human serum. There is no reactivity vs. non-activated normal human serum or plasma -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human C5b-9. Purified human SC5b-9 complex.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituent: 99.9% PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
This antibody is provided as IgG fraction. Please note that the concentration value refers to the fraction. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab55811の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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ICC/IF | (2) |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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関連性
Activation of the complement system plays a key role in normal inflammatory response to injury but may cause substantial injury when activated inappropriately. The complement system is activated either through the classical (antibody induced) or the alternative (microbial surface, polysacharride induced) pathway, both leading to the formation of the C5b9 complex. Fluid phase binding of the multifunctional glycoprotein S protein (vitronectin) to C5b9 leads to the formation of a cytolytically inactive complex, SC5b9, which is unable to attach to cells. -
細胞内局在
Secreted -
参照データベース
- Entrez Gene: 727 Human
- Entrez Gene: 729 Human
- Entrez Gene: 730 Human
- Entrez Gene: 731 Human
- Entrez Gene: 735 Human
- SwissProt: P01031 Human
- SwissProt: P02748 Human
- SwissProt: P07357 Human
see all -
別名
- C5 antibody
- c5b 9 antibody
- C6 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5b-9 antibody (ab55811)ab55811 staining C5b-9 in human liver.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required. -
ICC/IF image of ab55811 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55811, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (82)
ab55811 は 82 報の論文で使用されています。
- Wang Z et al. Endothelial-derived complement factor D contributes to endothelial dysfunction in malignant nephrosclerosis via local complement activation. Hypertens Res 46:1759-1770 (2023). PubMed: 37188751
- Giang J et al. Successful pharmacological intervention at different levels of the complement system in an in vitro complement fixation model for bullous pemphigoid. Exp Dermatol 32:632-640 (2023). PubMed: 36704908
- Seifert L et al. The classical pathway triggers pathogenic complement activation in membranous nephropathy. Nat Commun 14:473 (2023). PubMed: 36709213
- Yamasaki Y et al. Macroglossia in rapidly progressive inclusion body myositis. Neuropathology 43:252-256 (2023). PubMed: 36349419
- Yang W et al. Mice 3D testicular organoid system as a novel tool to study Zika virus pathogenesis. Virol Sin 38:66-74 (2023). PubMed: 36241087