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AB303662

Anti-C5a / C5a des Arg 抗体 [C17/5]

Anti-C5a / C5a des Arg antibody [C17/5]

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Mouse Recombinant Monoclonal C5 antibody. Suitable for WB, I-ELISA and reacts with Human, Recombinant full length protein - Human samples.

別名を表示する

CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4

3 Images
Indirect ELISA - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)

Indirect ELISA (dose response curve) of antigen Human C5a at a concentration of 5000 ng/ml. Primary antibody ab303662 used at 0-2000 ng/ml. Secondary antibody was a Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) used at 1/1000 dilution.

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)
  • WB

Supplier Data

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile observed is consistent with what has been described in the literature (PMID : 1710957). Very low or no expression of C5 alpha chain is observed in skeletal muscle. 110 kDa Complement C5 alpha chain is observed. Exposure time : 60 seconds

All lanes:

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (ab303662) at 1/1000 dilution

Lane 1:

Human plasma lysate at 20 µg

Lane 2:

Human skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG Fc (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-mouse-igg-fc-hrp-preadsorbed-ab98717'>ab98717</a>) at 1/5000 dilution

Observed band size: 110 kDa

false

Exposure time: 60s

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)
  • WB

Supplier Data

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (AB303662)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-C5a / C5a des Arg antibody [C17/5] (ab303662) at 1/1000 dilution

Lane 1:

Human C5 Recombinant protein at 40 ng

Lane 2:

Human C5a Recombinant protein at 40 ng

Secondary

All lanes:

Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

Observed band size: 10 kDa,110 kDa

true

Exposure time: 3min

関連する標識済み抗体及び組成の異なる製品 (1)

  • Carrier free

    Anti-C5a / C5a des Arg antibody [C17/5] - BSA and Azide Free

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

C17/5

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

I-ELISA, WB

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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組合せ製品

Assay Kits

AB125963

Human Complement C5 ELISA Kit

Sandwich ELISA - Human Complement C5 ELISA Kit (AB125963)

  • 0
  • 0
Primary Antibodies

AB200999

Anti-C3 antibody [EPR19394]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-C3 antibody [EPR19394] (AB200999)

  • 4
  • 9
Primary Antibodies

AB55811

Anti-C5b-9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5b-9 antibody (AB55811)

  • 3
  • 4
Primary Antibodies

AB252435

Anti-C5a-R antibody [EPR23278-117]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-C5a-R antibody [EPR23278-117] (AB252435)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }
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製品の詳細

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein A
バッファー組成
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
出荷温度
Blue Ice
短期保存期間
1-2 weeks
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The C5a protein also known as anaphylatoxin C5a is a small potent molecule weighing approximately 11 kDa. It originates from the larger complement component C5 during complement system activation. The C5a des Arg is a slightly modified version produced when enzymes cleave the C-terminal arginine from C5a. Both forms express on cell surfaces and recognize specific receptors on target cells particularly in immune and inflammatory responses.
Biological function summary

This protein controls complex interactions within the immune system specifically as part of the cleavage product of C5 a component of the complement system. C5a binds to receptors such as C5aR and C5L2 which appear in high numbers on various immune cells like macrophages neutrophils and endothelial cells. This binding triggers multiple intracellular pathways mediating inflammatory processes and immune cell activation.

Pathways

The complement system involves the activation sequences where C5a plays a pivotal role. It participates in the activation of the classic and the alternative complement pathways contributing to the opsonization and lysis of pathogens. C5a works closely with proteins such as C3a and C9 orchestrating the body's defense mechanisms against infections while facilitating communication between the innate and adaptive immune systems.

C5a plays a notable role in inflammatory diseases like rheumatoid arthritis and sepsis. Its overactivation relates to severe inflammatory responses and tissue damage. The link between C5a and other inflammatory mediators such as tumor necrosis factor-alpha (TNF-alpha) emphasizes its contribution to the pathogenesis of these conditions. Understanding these pathways offers insight into potential therapeutic targets for modulating excessive inflammation and immune responses.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 6554279). Activated downstream of classical, alternative, lectin and GZMK complement pathways (PubMed : 12878586, PubMed : 18204047, PubMed : 30643019, PubMed : 39914456, PubMed : 39814882, PubMed : 6554279).. Complement C5b. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis (PubMed : 26841837, PubMed : 27052168, PubMed : 30552328, PubMed : 30643019). Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex : C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9 (PubMed : 30552328, PubMed : 30643019). During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending (PubMed : 30552328, PubMed : 32569291).. C5a anaphylatoxin. Mediator of local inflammatory process released following cleavage by C5 convertase (PubMed : 8182049, PubMed : 9553099). Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed : 36806352, PubMed : 37852260, PubMed : 37169960, PubMed : 8182049, PubMed : 9553099). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation (PubMed : 342601, PubMed : 37852260, PubMed : 37169960, PubMed : 5765461, PubMed : 8182049, PubMed : 9553099).
See full target information C5
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Abcam product promise

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