Anti-C5a / C5a des Arg 抗体 [2942] (ab11877)
Key features and details
- Mouse monoclonal [2942] to C5a / C5a des Arg
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-C5a / C5a des Arg antibody [2942]
C5a / C5a des Arg 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2942] to C5a / C5a des Arg -
由来種
Mouse -
特異性
This antibody reacts with a neo-epitope on C5a that is formed upon cleavage of C5 into C5a and C5b. It will detect C5a and C5a des-Arg (Not C5). -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Eye tissue of patient with age-related macular degeneration AMD IF/ICC: HeLa cell line.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
0.2 µm filtered -
ポリ/モノ
モノクローナル -
クローン名
2942 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11877の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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関連性
C5 is involved in the activation of the lytic pathway within the complement system, which is an important factor in innate immunity. -
参照データベース
- Entrez Gene: 727 Human
- Omim: 120900 Human
- SwissProt: P01031 Human
- SwissProt: Q14CJ0 Human
- SwissProt: Q27I61 Human
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別名
- Anaphylatoxin C5a analog antibody
- C5 antibody
- C5a anaphylatoxin antibody
see all
画像
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ICC/IF image of ab11877 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11877, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C5a / C5a des Arg antibody [2942] (ab11877)IHC image of ab11877 staining in normal human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab11877, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab11877 は 6 報の論文で使用されています。
- Cho YT et al. Clinical, Histopathologic, and Immunohistochemical Features of Patients with IgG/IgA Pemphigus. Biomedicines 10:N/A (2022). PubMed: 35625932
- Deng S et al. Anaphylatoxins orchestrate Th17 response via interactions between CD16+ monocytes and pleural mesothelial cells in tuberculous pleural effusion. PLoS Negl Trop Dis 15:e0009508 (2021). PubMed: 34237073
- Corvillo F et al. Evidence of ongoing complement activation on adipose tissue from an 11-year-old girl with Barraquer-Simons syndrome. J Dermatol 47:1439-1444 (2020). PubMed: 32890433
- Neri T et al. Human pre-valvular endocardial cells derived from pluripotent stem cells recapitulate cardiac pathophysiological valvulogenesis. Nat Commun 10:1929 (2019). PubMed: 31028265
- Kajikawa T et al. Safety and Efficacy of the Complement Inhibitor AMY-101 in a Natural Model of Periodontitis in Non-human Primates. Mol Ther Methods Clin Dev 6:207-215 (2017). IHC-P ; Cynomolgus monkey . PubMed: 28879212
- Nozaki M et al. Drusen complement components C3a and C5a promote choroidal neovascularization. Proc Natl Acad Sci U S A 103:2328-33 (2006). PubMed: 16452172