Anti-C4orf48 抗体 (ab185315)
Key features and details
- Rabbit polyclonal to C4orf48
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-C4orf48 antibody -
製品の詳細
Rabbit polyclonal to C4orf48 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant fragment corresponding to Human C4orf48 aa 1 to the C-terminus.
Database link: Q5BLP8 -
ポジティブ･コントロール
- IHC-P: Human cerebral cortex and skin tissue; ICC: PC-3 whole cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab185315の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100 |
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| IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 特記事項 |
|---|
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100 |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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細胞内局在
Secreted -
参照データベース
- Entrez Gene: 615222. Cow
- Entrez Gene: 401115 Human
- Entrez Gene: 381633 Mouse
- Omim: 614690 Human
- SwissProt: A0JNN8 Cow
- SwissProt: Q5BLP8 Human
- SwissProt: Q3UR78 Mouse
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別名
- Gm1673 antibody
- Neuropeptide-like protein C4orf48 antibody
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4orf48 antibody (ab185315)
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling C4orf48 in the cytoplasm of neuronal cells with ab185315 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence - Anti-C4orf48 antibody (ab185315)Immunocytochemistry analysis of PC-3 (human prostate adenocarcinoma cell line) whole cells labeling C4orf48 in the cytosol with ab185315 at 2 µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4orf48 antibody (ab185315)Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling C4orf48 in the cytoplasm of neuronal cells with ab185315 at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab185315 は 1 報の論文で使用されています。
- Zhang S et al. Mitochondrial peptide BRAWNIN is essential for vertebrate respiratory complex III assembly. Nat Commun 11:1312 (2020). PubMed: 32161263