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リコンビナントRabMAb

Anti-C4 binding protein/C4BPB 抗体 [EPR17101] (ab199430)

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Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
  • Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
  • Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
  • Immunocytochemistry/ Immunofluorescence - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
  • Immunoprecipitation - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
  • Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17101] to C4 binding protein/C4BPB
  • Suitable for: IHC-P, WB, IP, ICC/IF
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-C4 binding protein/C4BPB antibody [EPR17101]
    C4 binding protein/C4BPB 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR17101] to C4 binding protein/C4BPB

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-P, WB, IP, ICC/IFmore details

  • 種交差性

    交差種: Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Human plasma and human ovary cancer cell extracts. Human serum. Rat plasma. IHC-P: Human hepatocellular carcinoma and human bladder carcinoma tissue. Rat liver tissue. ICC/IF: HepG2 cells. IP: Human serum.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR17101

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab199430の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 28 kDa).
IP
1/80.
ICC/IF
1/250.
特記事項
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 28 kDa).
IP
1/80.
ICC/IF
1/250.

ターゲット情報

  • 機能

    Controls the classical pathway of complement activation. It binds as a cofactor to C3b/C4b inactivator (C3bINA), which then hydrolyzes the complement fragment C4b. It also accelerates the degradation of the C4bC2a complex (C3 convertase) by dissociating the complement fragment C2a. It also interacts with anticoagulant protein S and with serum amyloid P component. The beta chain binds protein S.

  • 配列類似性

    Contains 3 Sushi (CCP/SCR) domains.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C4b binding protein beta chain antibody
      • C4b-binding protein beta chain antibody
      • C4BP antibody
      • C4BPB antibody
      • C4BPB_HUMAN antibody
      • Complement component 4 binding protein beta antibody
      • Complement component 4 binding protein beta chain antibody
      • Complement component 4-binding protein beta antibody
      • OTTHUMP00000034288 antibody
      • OTTHUMP00000034289 antibody
      • OTTHUMP00000034375 antibody
      • OTTHUMP0000003474 antibody
      • Proline rich protein antibody
      • PRP antibody
      see all

    画像

    • Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      All lanes : Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/1000 dilution

      Lane 1 : Human plasma
      Lane 2 : Human ovary cancer extract.

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 28 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking/dilution buffer: 5% NFDM/TBST.

       

      The observed MW is consistent with what has been described in the literature (PMID: 2300577)

    • Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/10000 dilution + Human serum at 20 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 28 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?


      Exposure time: 1 minute


      Blocking/dilution buffer: 5% NFDM/TBST.

      The observed MW is consistent with what has been described in the literature (PMID: 2300577)

    • Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      Western blot - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430) at 1/1000 dilution + Rat plasma at 10 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 28 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?


      Exposure time: 30 seconds


      Blocking/dilution buffer: 5% NFDM/TBST.

      The observed MW is consistent with what has been described in the literature (PMID: 2300577)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

      Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human hepatocellular carcinoma tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

      Immunohistochemical analysis of paraffin-embedded Human bladder carcinoma tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Plasma staining on Human bladder carcinoma tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

      Immunohistochemical analysis of paraffin-embedded Rat liver tissue labeling C4 binding protein/C4BPB with ab199430 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on rat liver tissue is observed. Counter stained with Hematoxylin.
      Negative control: Used PBS instead of primary ab, secondary ab is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      Immunocytochemistry/ Immunofluorescence - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma) cells labeling C4 binding protein/C4BPB with ab199430 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Confocal image showing cytoplasm staining on HepG2 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
      The negative controls are as follows:-
      -ve control 1 - ab199430 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
      -ve control 2. - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

    • Immunoprecipitation - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      Immunoprecipitation - Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

      C4 binding protein/C4BPB was immunoprecipitated from 1mg of Human serum with ab199430 at 1/80 dilution. Western blot was performed from the immunoprecipitate using ab199430 at 1/10000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Lane 1: Human serum 10 µg. Lane 2: Human serum following immunoprecipitation. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199430 in Human serum.
      Blocking and dilution buffer and concentration: 5% NFDM/TBST. Exposure: 10 seconds.

    • Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)
      Anti-C4 binding protein/C4BPB antibody [EPR17101] (ab199430)

    参考文献 (1)

    ab199430 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab199430 は 1 報の論文で使用されています。

    • Kobayashi S  et al. Collagen type I-mediated mechanotransduction controls epithelial cell fate conversion during intestinal inflammation. Inflamm Regen 42:49 (2022). PubMed: 36443773

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