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AB11863

Anti-C4 抗体 [16D2]

Anti-C4 antibody [16D2]

4

(1 Review)

|

(13 Publications)

Rat Monoclonal C4 antibody. Suitable for IHC-Fr and reacts with Mouse samples. Cited in 13 publications.

別名を表示する

C4, C4b, Complement C4-B

1 Images
Immunohistochemistry (Frozen sections) - Anti-C4 antibody [16D2] (AB11863)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-C4 antibody [16D2] (AB11863)

Frozen section of mouse pulmonary artery of control (left) and mouse with hypoxia-induced pulmonary hypertnetion (right). Staining of C4 (red) with ab11863 in a dilution 1/300. Green is autofluorescence of elastic lamellae defining vascular media. Blue is cell nucleus.

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

16D2

アイソタイプ

IgG2a

キャリアフリー

No

交差種

Mouse

アプリケーション

IHC-Fr

applications

特異性

The monoclonal antibody 16D2 reacts with intact mouse C4, C4b and C4d.

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Reactivity data

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下記製品にもご興味をお持ちいただけるかもしれません:

AB277717

Human C4b ELISA Kit (Complement C4-B)

0

0 Reviews

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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
バッファー組成
Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Complement component 4 (C4) is a significant protein in the complement system also known as C4 complement. C4 has a molecular mass of approximately 206 kDa and mainly expresses in the liver. It is present in the blood plasma. C4 plays an important role in the immunological response and activates classical and lectin complement pathways. It serves a mechanical function by binding to pathogen surfaces and facilitating the cleavage that leads to the downstream activation of the complement cascade. There's a C4 ELISA test that allows for quantifying C4 levels in serum which is important for clinical diagnostics.
Biological function summary

Complement C4 participates in the immune defense by marking pathogens for destruction and enhancing phagocytosis. C4 is a part of a complex with other complement proteins like C3 and C1 to initiate the formation of the C3 convertase enzyme. This action amplifies the immune response leading to the clearance of microorganisms and apoptotic cells. C4 also plays a role in regulating the inflammation process maintaining homeostasis in the immune system.

Pathways

C4 fits into the complement activation pathway specifically the classical and lectin pathways. These pathways are important for innate immunity and connect with components like complement C3 and complement C1. Through the classical pathway C4 gets activated after binding to antibodies that have recognized antigens leading to a chain reaction that strengthens the immune response. The lectin pathway also requires C4 activation through different stimuli solidifying C4's central role in immune system signaling networks.

Abnormal C4 activity associates with autoimmune diseases like systemic lupus erythematosus (SLE) and rheumatoid arthritis. Low levels of complement C4 in serum can indicate these autoimmune conditions where the immune system mistakenly attacks its own tissues. C4 also connects to other proteins like complement component C1q in the context of SLE. C4 deficiencies or abnormalities may contribute to the development or exacerbation of these diseases highlighting its importance in disease pathogenesis and highlighting its value as a target for diagnostic tests such as C4 ELISA or serum complement tests.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

Non-enzymatic component of C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. Catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens (By similarity).
See full target information C4b
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文献 (13)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed34502128

2021

Sodium Iodate-Induced Degeneration Results in Local Complement Changes and Inflammatory Processes in Murine Retina.

Applications

Unspecified application

Species

Unspecified reactive species

Anne Enzbrenner,Rahel Zulliger,Josef Biber,Ana Maria Quintela Pousa,Nicole Schäfer,Corinne Stucki,Nicolas Giroud,Marco Berrera,Elod Kortvely,Roland Schmucki,Laura Badi,Antje Grosche,Diana Pauly,Volker Enzmann

Infection and immunity 87: PubMed31182619

2019

Persistent Infection of the Brain Induced Neurodegeneration Associated with Activation of Complement and Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Li,Emily G Severance,Raphael P Viscidi,Robert H Yolken,Jianchun Xiao

Brain, behavior, and immunity 80:889-899 PubMed31158497

2019

Microglial cell hyper-ramification and neuronal dendritic spine loss in the hippocampus and medial prefrontal cortex in a mouse model of PTSD.

Applications

Unspecified application

Species

Unspecified reactive species

Kristie Leigh Smith,Mustafa S Kassem,David J Clarke,Michael P Kuligowski,Miguel A Bedoya-Pérez,Stephanie M Todd,Jim Lagopoulos,Maxwell R Bennett,Jonathon C Arnold

Frontiers in immunology 9:3038 PubMed30728823

2019

Germinal Center Alloantibody Responses Mediate Progression of Chronic Allograft Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Manu Chhabra,Jawaher Alsughayyir,M Saeed Qureshi,Mekhola Mallik,Jason M Ali,Ivonne Gamper,Ellen L Moseley,Sarah Peacock,Vasilis Kosmoliaptsis,Martin J Goddard,Michelle A Linterman,Reza Motallebzadeh,Gavin J Pettigrew

Cell reports 15:1214-27 PubMed27134179

2016

Augmentation of Recipient Adaptive Alloimmunity by Donor Passenger Lymphocytes within the Transplant.

Applications

Unspecified application

Species

Unspecified reactive species

Ines G Harper,Jason M Ali,Simon J F Harper,Elizabeth Wlodek,Jawaher Alsughayyir,Margaret C Negus,M Saeed Qureshi,Reza Motalleb-Zadeh,Kourosh Saeb-Parsy,Eleanor M Bolton,J Andrew Bradley,Menna R Clatworthy,Thomas M Conlon,Gavin J Pettigrew

American journal of transplantation : official jou 14:2263-2274 PubMed25155089

2014

Depletion of foxp3(+) T cells abrogates tolerance of skin and heart allografts in murine mixed chimeras without the loss of mixed chimerism.

Applications

Unspecified application

Species

Unspecified reactive species

K Shinoda,T Akiyoshi,C M Chase,E A Farkash,D K Ndishabandi,C M Raczek,D P Sebastian,P Della Pelle,P S Russell,J C Madsen,R B Colvin,A Alessandrini

Human molecular genetics 23:52-68 PubMed23943789

2013

Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration.

Applications

Unspecified application

Species

Mouse

Donita L Garland,Rosario Fernandez-Godino,Inderjeet Kaur,Kaye D Speicher,James M Harnly,John D Lambris,David W Speicher,Eric A Pierce

Diabetes 62:3143-50 PubMed23852699

2013

Transient B-cell depletion combined with apoptotic donor splenocytes induces xeno-specific T- and B-cell tolerance to islet xenografts.

Applications

Unspecified application

Species

Unspecified reactive species

Shusen Wang,James Tasch,Taba Kheradmand,Jodie Ulaszek,Sora Ely,Xiaomin Zhang,Bernhard J Hering,Stephen D Miller,Xunrong Luo

PLoS pathogens 8:e1002867 PubMed22912582

2012

Lymphotoxin, but not TNF, is required for prion invasion of lymph nodes.

Applications

Unspecified application

Species

Unspecified reactive species

Tracy O'Connor,Nathalie Frei,Jana Sponarova,Petra Schwarz,Mathias Heikenwalder,Adriano Aguzzi

The Journal of pathology 225:502-11 PubMed21688269

2011

Classical complement activation as a footprint for murine and human antiphospholipid antibody-induced fetal loss.

Applications

IHC-Fr

Species

Mouse

Danielle Cohen,Aletta Buurma,Natascha N Goemaere,Guillermina Girardi,Saskia le Cessie,Sicco Scherjon,Kitty W M Bloemenkamp,Emile de Heer,Jan A Bruijn,Ingeborg M Bajema
View all publications
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