Anti-C3a / C3a des Arg 抗体 [2991] (ab11873)
Key features and details
- Mouse monoclonal [2991] to C3a / C3a des Arg
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-C3a / C3a des Arg antibody [2991]
C3a / C3a des Arg 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [2991] to C3a / C3a des Arg -
由来種
Mouse -
特異性
ab11873 reacts with a neo-epitope (des-Arg) on C3a that is formed when C3 is cleaved into C3a and C3b. ab11873 recognizes C3a and C3a des arg only. It has the most affinity with C3a des arg and least affinity with C3a. The des arg variant has an about 5x higher affinity than C3a. The antibody does not recognize C3b or full C3, as it recognizes a neo-epitope that is not available on C3. -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Other Immunogen Type corresponding to Human C3a/ C3a des Arg.
Database link: P01024 -
ポジティブ・コントロール
- Activated human serum or purified human C3adesArg protein
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
0.2 µm filtered -
ポリ/モノ
モノクローナル -
クローン名
2991 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab11873の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. |
ターゲット情報
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機能
C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.
Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. -
組織特異性
Plasma. -
関連疾患
Defects in C3 are the cause of complement component 3 deficiency (C3D) [MIM:613779]. A rare defect of the complement classical pathway. Patients develop recurrent, severe, pyogenic infections because of ineffective opsonization of pathogens. Some patients may also develop autoimmune disorders, such as arthralgia and vasculitic rashes, lupus-like syndrome and membranoproliferative glomerulonephritis.
Genetic variation in C3 is associated with susceptibility to age-related macular degeneration type 9 (ARMD9) [MIM:611378]. ARMD is a multifactorial eye disease and the most common cause of irreversible vision loss in the developed world. In most patients, the disease is manifest as ophthalmoscopically visible yellowish accumulations of protein and lipid that lie beneath the retinal pigment epithelium and within an elastin-containing structure known as Bruch membrane.
Defects in C3 are a cause of susceptibility to hemolytic uremic syndrome atypical type 5 (AHUS5) [MIM:612925]. An atypical form of hemolytic uremic syndrome. It is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea. In contrast to typical hemolytic uremic syndrome, atypical forms have a poorer prognosis, with higher death rates and frequent progression to end-stage renal disease. Note=Susceptibility to the development of atypical hemolytic uremic syndrome can be conferred by mutations in various components of or regulatory factors in the complement cascade system. Other genes may play a role in modifying the phenotype. -
配列類似性
Contains 1 anaphylatoxin-like domain.
Contains 1 NTR domain. -
翻訳後修飾
C3b is rapidly split in two positions by factor I and a cofactor to form iC3b (inactivated C3b) and C3f which is released. Then iC3b is slowly cleaved (possibly by factor I) to form C3c (beta chain + alpha' chain fragment 1 + alpha' chain fragment 2), C3dg and C3f. Other proteases produce other fragments such as C3d or C3g.
Phosphorylation sites are present in the extracelllular medium. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 718 Human
- Omim: 120700 Human
- SwissProt: P01024 Human
- Unigene: 529053 Human
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別名
- Acylation stimulating protein antibody
- Acylation stimulating protein cleavage product antibody
- ASP antibody
see all
データシートおよび資料
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Datasheet download
参考文献 (5)
ab11873 は 5 報の論文で使用されています。
- Addinsall AB et al. JAK/STAT inhibition augments soleus muscle function in a rat model of critical illness myopathy via regulation of complement C3/3R. J Physiol 599:2869-2886 (2021). PubMed: 33745126
- Yuan K et al. Complement C3 overexpression activates JAK2/STAT3 pathway and correlates with gastric cancer progression. J Exp Clin Cancer Res 39:9 (2020). PubMed: 31928530
- Kolev M et al. Diapedesis-Induced Integrin Signaling via LFA-1 Facilitates Tissue Immunity by Inducing Intrinsic Complement C3 Expression in Immune Cells. Immunity 52:513-527.e8 (2020). PubMed: 32187519
- Jiménez-Reinoso A et al. Human plasma C3 is essential for the development of memory B, but not T, lymphocytes. J Allergy Clin Immunol 141:1151-1154.e14 (2018). PubMed: 29113906
- Kemper C & Kolev M Enzymatic Reactions and Detection of C3 Cleavage Fragments. Bio Protoc 4:N/A (2014). PubMed: 29094056