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AB11874

Anti-C3 / C3a 抗体 [2898]

Anti-C3 / C3a antibody [2898]

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(1 Publication)

Mouse Monoclonal C3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human C3.

別名を表示する

CPAMD1, C3, Complement C3, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1

1 Images
Western blot - Anti-C3 / C3a antibody [2898] (AB11874)
  • WB

Unknown

Western blot - Anti-C3 / C3a antibody [2898] (AB11874)

All lanes:

Western blot - Anti-C3 / C3a antibody [2898] (ab11874) at 1/200 dilution

Lane 1:

HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 2:

Human liver tissue lysate - total protein (ab29889) at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 187 kDa

Observed band size: 115 kDa,187 kDa,71 kDa

false

Key facts

宿主種

Mouse

クローン性

Monoclonal

クローン番号

2898

アイソタイプ

IgG1

キャリアフリー

No

交差種

Human

アプリケーション

WB

applications

免疫原

Synthetic Peptide within Human C3. The exact immunogen used to generate this antibody is proprietary information.

P01024

特異性

This antibody reacts with the C-terminus of C3a. It reacts with intact C3 as with C3a.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10", "WB-species-notes": "<p><div> </div>" } } }
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出荷温度及び保存条件

製品の状態
Liquid
精製方法
Affinity purification Protein G
精製に関する特記事項
0.2 µm filtered
バッファー組成
Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA
出荷温度
Blue Ice
短期保存温度
+4°C
長期保存温度
-20°C
分注に関する情報
Upon delivery aliquot
保管に関する情報
Avoid freeze / thaw cycle
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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

The Complement Component 3 (C3) is a central protein in the complement system often referred to as C3 or C3a when discussing its active fragment. C3 weighs approximately 187 kDa and expresses widely in the liver then circulating in blood plasma. When activated C3 cleaves into C3a and C3b. C3a acts as an anaphylatoxin contributing to the immune response by promoting inflammation. C3 plays critical roles in immunity by enhancing phagocytosis and clearance of pathogens.
Biological function summary

C3 fragments particularly C3a influence various immune responses. As part of the complement system C3 integrates into a complex network of proteins. This activation cascade enhances the body's ability to combat pathogens. C3a interacts with receptors on immune cells mediating inflammatory response and acting as a chemoattractant. C3b another key fragment binds to pathogen surfaces aiding opsonization and immune clearance.

Pathways

C3's function plays an important role in the alternative and classical complement pathways. In the alternative pathway C3b contributes directly by forming a C3 convertase complex. This interaction facilitates further cleavage of C3 amplifying immune response. C3b also associates with proteins like Factor B and Factor D to stabilize the convertase complex and enhance opsonization. In the classical pathway C3 interacts with components like C4b to optimize pathogen elimination.

C3 and its fragments have significant connections to immune disorders and infections. Low levels or dysfunctional C3 can lead to susceptibility to bacterial infections. C3a has associations with asthma where it exacerbates the inflammatory response in lung tissues. In autoimmune diseases such as systemic lupus erythematosus (SLE) improper regulation of C3 activation leads to tissue damage. The interaction of C3 with proteins like C1q in SLE highlights the interplay between complement activation and autoimmune pathologies.
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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:
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ターゲットの情報

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2 (PubMed : 10432298, PubMed : 15833747, PubMed : 16333141, PubMed : 19615750, PubMed : 2909530, PubMed : 8376604, PubMed : 9059512).
See full target information C3
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular vision 17:2856-63 PubMed22128233

2011

Aqueous cytokine and growth factor levels do not reliably reflect those levels found in the vitreous.

Applications

WB

Species

Human

Stephanie M Ecker,Joshua C Hines,Scott M Pfahler,Bert M Glaser
View all publications
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