AB11862

Anti-C3 抗体 [11H9]

Name: Anti-C3 抗体 [11H9] (ab11862) | Abcam
Brand: Abcam Limited
SKU: AB11862
Rating: 4 (4 reviews)

Anti-C3 antibody [11H9]

(4 Reviews)

(55 Publications)

Anti-C3 antibody [11H9] (ab11862) is a rat monoclonal antibody detecting C3/C3b in ICC/IF. Suitable for Mouse.

- Over 50 publications
- Trusted since 2004

別名を表示する

Complement C3, HSE-MSF, C3

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [11H9] (AB11862)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [11H9] (AB11862)

ab11862 staining C3 in murine kidney cells by Immunocytochemistry/ Immunofluorescence.C3 protein fragments deposited on kidney cells of MPL-lpr mouse. Glomerular staining pattern.

Key facts

宿主種

Rat

クローン性

Monoclonal

クローン番号

11H9

アイソタイプ

IgG2a

キャリアフリー

交差種

Mouse

アプリケーション

ICC/IF

applications

免疫原

Cell preparation containing C3 protein. The exact immunogen used to generate this antibody is proprietary information.

P01027

特異性

This antibody recognizes both intact C3 and its cleaved products C3b, iC3b, C3d and C3dg.The mature protein C3 has a molecular weight of approximately 190 kDa. The complement factor C3 consists of an alpha- and a beta-chain, linkedby disulfide bond. C3 convertase activates C3 by cleaving the alpha chain, releasing C3a anaphylotoxin and generating C3b (alpha chain and beta chain). C3b has a molecular weight of approximately 185 kDa. C3b is rapidly split in two positions by factor I and a cofactor to formiC3b (inactivated C3b) and C3f which is released. iC3b has a molecular weight of approximately 182 kDa. Does not cross react with C4.

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Reactivity data

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製品の詳細

What is this antibody validated in?
Anti-C3 antibody [11H9] (ab11862) is a rat monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Trusted by the scientific community
Anti-C3 [11H9] (ab11862) was first used in a scientific publication in 2004 and has been cited over 50 times in peer-reviewed journals.

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出荷温度及び保存条件

製品の状態

Liquid

精製方法

Affinity purification Protein G

精製に関する特記事項

0.2 µm filtered

バッファー組成

Preservative: 0.02% Sodium azide Constituents: PBS, 0.1% BSA

出荷温度

Blue Ice

短期保存温度

+4°C

長期保存温度

-20°C

分注に関する情報

Upon delivery aliquot

保管に関する情報

Avoid freeze / thaw cycle

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

Complement component 3 (C3) commonly known as C3 complement is a central protein in the complement system which plays a significant role in immune response. C3b a fragment of C3 is produced when C3 undergoes cleavage. C3 is a large protein with a mass of approximately 185 kDa. The liver primarily secretes C3 into the bloodstream. It circulates in the plasma and is found in high concentration making it one of the most abundant components of the complement system.

Biological function summary

Complement component C3 forms part of the innate immune system by promoting opsonization which enhances phagocytosis of pathogens. C3b binds to pathogens' surfaces facilitating their recognition by phagocytes. C3 as part of a complex with C3 convertase also has a role in amplifying the activation of the complement cascade. The proteolytic cleavage of C3 into C3b and C3a leads to the activation of other components forming the membrane attack complex and orchestrating inflammation.

Pathways

The complement component C3 functions within both the classical and alternative complement pathways. It acts as a convergence point where the complement activation pathways meet. C3 is activated into C3b and C3a which are key to amplifying the cascade. Furthermore C3 interacts with proteins such as factor B and factor D in the alternative pathway and C4 and C2 in the classical pathway facilitating the formation of C3 convertase necessary for pathway progression.

Complement C3 shows associations with immune-related and inflammatory diseases. Deficiencies or malfunctions of complement C3 can lead to increased susceptibility to infections due to impaired opsonization and clearance of pathogens. Additionally overactivation of the complement system involving C3 can contribute to autoimmune disorders such as systemic lupus erythematosus. Other proteins linked to these diseases include C4 in lupus and factor H in age-related macular degeneration which controls complement pathway activation.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.. Derived from proteolytic degradation of complement C3, C3a anaphylatoxin is a mediator of local inflammatory process. In chronic inflammation, acts as a chemoattractant for neutrophils (By similarity). It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes. The short isoform has B-cell stimulatory activity.. C3-beta-c. Acts as a chemoattractant for neutrophils in chronic inflammation.. Acylation stimulating protein. Adipogenic hormone that stimulates triglyceride (TG) synthesis and glucose transport in adipocytes, regulating fat storage and playing a role in postprandial TG clearance. Appears to stimulate TG synthesis via activation of the PLC, MAPK and AKT signaling pathways. Ligand for C5AR2. Promotes the phosphorylation, ARRB2-mediated internalization and recycling of C5AR2.

See full target information C3

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文献 (55)

Recent publications for all applications. Explore the full list and refine your search

Glia 71:2418-2436 PubMed37395288

2023

Knockout of microglial Hv1 proton channel reduces neurotoxic A1 astrocytes and neuronal damage via the ROS/STAT3 pathway after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Li,Yi Xie,Rui Liu,Ziyue Wang,Peng Chen,Minghuan Wang,Zhiyuan Yu,Wei Wang,Xiang Luo

The Journal of comparative neurology 531:1147-1162 PubMed37146123

2023

The effects of voluntary running exercise on the astrocytes of the medial prefrontal cortex in APP/PS1 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mei Liu,Lin Zhu,Yi-Jing Guo,Shan-Shan Zhang,Lin Jiang,Yi Zhang,Feng-Lei Chao,Yong Tang

Cell reports methods 3:100370 PubMed36814843

2023

A porcine islet-encapsulation device that enables long-term discordant xenotransplantation in immunocompetent diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kumiko Ajima,Naoto Tsuda,Tadashi Takaki,Shoji Furusako,Shigeki Matsumoto,Koya Shinohara,Yzumi Yamashita,Sayaka Amano,Chinatsu Oyama,Masayuki Shimoda

Journal of neuroinflammation 20:44 PubMed36823628

2023

The critical role of KLF4 in regulating the activation of A1/A2 reactive astrocytes following ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Wang,Longxuan Li

Virologica Sinica 38:66-74 PubMed36241087

2022

Mice 3D testicular organoid system as a novel tool to study Zika virus pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yang,Chen Zhang,Yan-Hua Wu,Li-Bo Liu,Zi-Da Zhen,Dong-Ying Fan,Zheng-Ran Song,Jia-Tong Chang,Pei-Gang Wang,Jing An

Nature communications 13:5786 PubMed36184639

2022

The alarmin interleukin-1α triggers secondary degeneration through reactive astrocytes and endothelium after spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Floriane Bretheau,Adrian Castellanos-Molina,Dominic Bélanger,Maxime Kusik,Benoit Mailhot,Ana Boisvert,Nicolas Vallières,Martine Lessard,Matthias Gunzer,Xiaoyu Liu,Éric Boilard,Ning Quan,Steve Lacroix

Frontiers in cellular neuroscience 16:862918 PubMed36003141

2022

Seed-induced Aβ deposits in the corpus callosum disrupt white matter integrity in a mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Aires,Stephanie Ziegler-Waldkirch,Marina Friesen,Wilfried Reichardt,Daniel Erny,Desiree Loreth,Andrew Harborne,Oliver Kretz,Dominik von Elverfeldt,Melanie Meyer-Luehmann

Journal of neuroinflammation 19:142 PubMed35690821

2022

Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yeonggwang Hwang,Hyoung-Chun Kim,Eun-Joo Shin

JCI insight 7: PubMed35167491

2022

Trastuzumab/pertuzumab combination therapy stimulates antitumor responses through complement-dependent cytotoxicity and phagocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Chung Tsao,Erika J Crosby,Timothy N Trotter,Junping Wei,Tao Wang,Xiao Yang,Amanda N Summers,Gangjun Lei,Christopher A Rabiola,Lewis A Chodosh,William J Muller,Herbert Kim Lyerly,Zachary C Hartman

Cancer discovery 12:1314-1335 PubMed35262173

2022

Melanoma-Secreted Amyloid Beta Suppresses Neuroinflammation and Promotes Brain Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Kevin Kleffman,Grace Levinson,Indigo V L Rose,Lili M Blumenberg,Sorin A A Shadaloey,Avantika Dhabaria,Eitan Wong,Francisco Galán-Echevarría,Alcida Karz,Diana Argibay,Richard Von Itter,Alfredo Floristán,Gillian Baptiste,Nicole M Eskow,James A Tranos,Jenny Chen,Eleazar C Vega Y Saenz de Miera,Melissa Call,Robert Rogers,George Jour,Youssef Zaim Wadghiri,Iman Osman,Yue-Ming Li,Paul Mathews,Ronald B DeMattos,Beatrix Ueberheide,Kelly V Ruggles,Shane A Liddelow,Robert J Schneider,Eva Hernando

View all publications

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Abcam product promise

当社は、高品質な試薬を通じてお客様の研究を力強くサポートすることをお約束いたします。ご使用いただく各段階で、常にお客様をサポートできる体制を整えております。万が一、製品が期待通りに機能しない場合は、「Abcam Product Promise」による当社保証制度に基づき、安心してご利用いただけます。

保証に関する詳細については利用規約をご確認ください。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-C3 抗体 [11H9]

Anti-C3 antibody [11H9]

Immunocytochemistry/ Immunofluorescence - Anti-C3 antibody [11H9] (AB11862)

Key facts

宿主種

クローン性

クローン番号

アイソタイプ

キャリアフリー

交差種

アプリケーション

免疫原

特異性

Reactivity data

製品の詳細

出荷温度及び保存条件

製品の状態

精製方法

精製に関する特記事項

バッファー組成

出荷温度

短期保存温度

長期保存温度

分注に関する情報

保管に関する情報

補足情報

Biological function summary

Pathways

製品プロトコール

ターゲットの情報

文献 (55)

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