Anti-C1s 抗体 [EPR9066(B)]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1s antibody [EPR9066(B)] (AB134943)

Immunohistochemical analysis of paraffin-embedded formalin-fixed Human liver tissue labelling C1s using ab134943 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IP

Unknown

Immunoprecipitation - Anti-C1s antibody [EPR9066(B)] (AB134943)

Western blot analysis on Immunoprecipitation pellet from human plasma lysate using anti-C1s RabMAb (ab134943).

All lanes:

Immunoprecipitation - Anti-C1s antibody [EPR9066(B)] (ab134943)

Predicted band size: 63 kDa,77 kDa

Observed band size: 65 kDa

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• WB

Unknown

Western blot - Anti-C1s antibody [EPR9066(B)] (AB134943)

All lanes:

Western blot - Anti-C1s antibody [EPR9066(B)] (ab134943) at 1/1000 dilution

Lane 1:

Human fetal kidney lysate at 10 µg

Lane 2:

A431 cell lysate at 10 µg

Lane 3:

Human platelet lysate at 10 µg

Lane 4:

Human plasma lysate at 10 µg

Lane 5:

Human serum lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 77 kDa

true

• WB

Lab

Western blot - Anti-C1s antibody [EPR9066(B)] (AB134943)

Western blot : Rabbit Monoclonal[EPR9066(B)] to C1s ab134943 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 77 kDa in Wild-type A549 cell lysates with no signal observed at this size in C1S knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-C1s antibody [EPR9066(B)] (ab134943) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

C1S knockout A549 at 20 µg

Lane 3:

HEK-293 at 20 µg

Lane 4:

Human Liver at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 77 kDa

Observed band size: 77 kDa

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