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リコンビナントRabMAb

Anti-C1r 抗体 [EPR14915] (ab185212)

Reviews (1) Submit a question References (2)
Western blot - Anti-C1r antibody [EPR14915] (ab185212)
  • Immunoprecipitation - Anti-C1r antibody [EPR14915] (ab185212)
  • Anti-C1r antibody [EPR14915] (ab185212)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14915] to C1r
  • Suitable for: IP, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-C1r antibody [EPR14915]
    C1r 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR14915] to C1r

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IP, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Human plasma lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR14915

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab185212の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IP
1/40 - 1/60.
WB (1)
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).

This antibody may be used diluted 1:2000 (PMID: 31749804).
特記事項
IP
1/40 - 1/60.
WB
1/1000 - 1/10000. Detects a band of approximately 85 kDa (predicted molecular weight: 80 kDa).

This antibody may be used diluted 1:2000 (PMID: 31749804).

ターゲット情報

  • 機能

    C1r B chain is a serine protease that combines with C1q and C1s to form C1, the first component of the classical pathway of the complement system.

  • 配列類似性

    Belongs to the peptidase S1 family.
    Contains 2 CUB domains.
    Contains 1 EGF-like domain.
    Contains 1 peptidase S1 domain.
    Contains 2 Sushi (CCP/SCR) domains.

  • 翻訳後修飾

    The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C1 R antibody
      • C1R antibody
      • C1R_HUMAN antibody
      • Complement C1r antibody
      • Complement C1r subcomponent antibody
      • Complement C1r subcomponent light chain antibody
      • Complement C1r subcomponent precursor antibody
      • Complement component 1 r subcomponent antibody
      • Complement component 1 subcomponent r antibody
      see all

    画像

    • Western blot - Anti-C1r antibody [EPR14915] (ab185212)
      Western blot - Anti-C1r antibody [EPR14915] (ab185212)
      Anti-C1r antibody [EPR14915] (ab185212) at 1/5000 dilution + Human plasma lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 80 kDa

    • Immunoprecipitation - Anti-C1r antibody [EPR14915] (ab185212)
      Immunoprecipitation - Anti-C1r antibody [EPR14915] (ab185212)

      Western blot analysis of immunoprecipitation pellet from Human plasma lysate immunoprecipitated using ab185212 at 1/50 dilution (lane 1) or PBS control (lane 2).
      Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    • Anti-C1r antibody [EPR14915] (ab185212)
      Anti-C1r antibody [EPR14915] (ab185212)

    参考文献 (2)

    ab185212 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab185212 は 2 報の論文で使用されています。

    • Amberger A  et al. Degradation of collagen I by activated C1s in periodontal Ehlers-Danlos Syndrome. Front Immunol 14:1157421 (2023). PubMed: 36960056
    • Gröbner R  et al. C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome. Front Immunol 10:2537 (2019). WB ; Human . PubMed: 31749804

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    Western blot abreview for Anti-C1r antibody [EPR14915]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (skin fibroblast)
    Gel Running Conditions
    Reduced Denaturing (10 % SDS gel)
    Loading amount
    20 µg
    Specification
    skin fibroblast
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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    Verified customer

    投稿 Nov 30 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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