AB92508

Anti-C1QB 抗体 [EPR2981]

Anti-C1QB antibody [EPR2981]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal C1QB antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications.

別名を表示する

Complement C1q subcomponent subunit B, C1QB

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

ab92508 showing positive staining in Normal liver tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

ab92508 showing positive staining in Normal brain tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

ab92508 showing positive staining in Normal spleen tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

ab92508 showing positive staining in Normal stomach tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

ab92508 at a 1/50 dilution staining C1QB in paraffin embedded Human kidney tissue by Immunohistochemistry.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QB antibody [EPR2981] (AB92508)

ab92508 showing positive staining in Normal breast tissue.

Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

Western blot - Anti-C1QB antibody [EPR2981] (AB92508)

WB

Unknown

Western blot - Anti-C1QB antibody [EPR2981] (AB92508)

All lanes:

Western blot - Anti-C1QB antibody [EPR2981] (ab92508) at 1/1000 dilution

All lanes:

Human plasma cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 27 kDa

false

関連する標識済み抗体及び組成の異なる製品 (1)

Carrier free

Anti-C1QB antibody [EPR2981] - BSA and Azide free

Key facts

宿主種

Rabbit

クローン性

Monoclonal

クローン番号

EPR2981

アイソタイプ

IgG

キャリアフリー

No

交差種

Human

アプリケーション

WB, IHC-P

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p>The use of an HRP/AP polymerized secondary antibody is recommended.</p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" } } }

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製品の詳細

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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出荷温度及び保存条件

製品の状態

Liquid

バッファー組成

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

出荷温度

Conditional Ambient

短期保存温度

+4°C

長期保存温度

-20°C

保管に関する情報

Stable for 12 months at -20°C

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補足情報

This supplementary information is collated from multiple sources and compiled automatically.

C1QB or complement component 1 q subcomponent beta chain is a protein that plays a mechanical role in the complement system. It has a mass of approximately 25 kDa and is expressed in many tissues including the liver and blood plasma. C1QB is a subunit of the C1q complex which is the first component of the classical complement pathway. This protein works with C1q's other subunits to recognize and bind to the Fc region of antibodies facilitating the activation of the complement pathway.

Biological function summary

C1QB is involved in the immune response and helps in the clearance of pathogens and apoptotic cells. It forms part of the C1q complex which includes two other components: C1QA and C1QC. This complex binds to antibodies that are attached to antigens on the surface of pathogens activating the C1r and C1s serine proteases. The activated enzymes then cleave other complement proteins triggering a cascade that results in opsonization inflammation and cell lysis.

Pathways

C1QB takes a significant role in the classical complement pathway and humoral immunity. The classical pathway starts with the binding of the C1 complex to antigen-antibody complexes. Through this mechanism C1QB associates with proteins such as C2 and C4 which further propagate the complement activation cascade. The classical complement pathway links to the lectin pathway where mannose-binding lectin substitutes for C1q to recognize pathogens.

Defects or deficiencies in C1QB relate to systemic lupus erythematosus (SLE) and hereditary angioedema. In SLE impaired function of C1QB leads to decreased clearance of immune complexes contributing to autoimmune reactions. This protein interacts with C1QA in disease mechanisms. In hereditary angioedema although C1QB is not directly defective its involvement in complement activation relates to mutations in C1 inhibitor protein. These mutations result in uncontrolled complement activation underlying the pathophysiology of the disorder.

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製品プロトコール

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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ターゲットの情報

Core component of the complement C1 complex, a multiprotein complex that initiates the classical pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 12847249, PubMed : 19006321, PubMed : 24626930, PubMed : 29449492, PubMed : 3258649, PubMed : 34155115, PubMed : 6249812, PubMed : 6776418). The classical complement pathway is initiated by the C1Q subcomplex of the C1 complex, which specifically binds IgG or IgM immunoglobulins complexed with antigens, forming antigen-antibody complexes on the surface of pathogens : C1QA, together with C1QB and C1QC, specifically recognizes and binds the Fc regions of IgG or IgM via its C1q domain (PubMed : 12847249, PubMed : 19006321, PubMed : 24626930, PubMed : 29449492, PubMed : 3258649, PubMed : 6776418). Immunoglobulin-binding activates the proenzyme C1R, which cleaves C1S, initiating the proteolytic cascade of the complement system (PubMed : 29449492). The C1Q subcomplex is activated by a hexamer of IgG complexed with antigens, while it is activated by a pentameric IgM (PubMed : 19706439, PubMed : 24626930, PubMed : 29449492). The C1Q subcomplex also recognizes and binds phosphatidylserine exposed on the surface of cells undergoing programmed cell death, possibly promoting activation of the complement system (PubMed : 18250442).

See full target information C1QB

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文献 (4)

Recent publications for all applications. Explore the full list and refine your search

European journal of immunology 55:e202451298 PubMed39508544

2024

Neutrophil Extracellular Traps Participate in the Pathogenesis of Lupus Through S100A10-Mediated Regulatory T-Cell Differentiation and Functional Abnormalities.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Guo,Qian Liang,Zhonghui Xue,Junling Yang,Pengyu Chen,Juan Ji,Jing Li,Genkai Guo,Haixia Cao,Xiaoqi Sha,Rui Zhao,Chen Dong,Zhifeng Gu

The EMBO journal 43:5494-5529 PubMed39390219

2024

Transplacental SARS-CoV-2 protein ORF8 binds to complement C1q to trigger fetal inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Tamiris Azamor,Débora Familiar-Macedo,Gielenny M Salem,Chineme Onwubueke,Ivonne Melano,Lu Bian,Zilton Vasconcelos,Karin Nielsen-Saines,Xianfang Wu,Jae U Jung,Feng Lin,Oluwatosin Goje,Edward Chien,Steve Gordon,Charles B Foster,Hany Aly,Ruth M Farrell,Weiqiang Chen,Suan-Sin Foo

eLife 13: PubMed38980810

2024

Tumor purity-related genes for predicting the prognosis and drug sensitivity of DLBCL patients.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenbang Ye,Ning Huang,Yongliang Fu,Rongle Tian,Liming Wang,Wenting Huang

Nature communications 15:1667 PubMed38396116

2024

Profiling of microglia nodules in multiple sclerosis reveals propensity for lesion formation.

Applications

Unspecified application

Species

Unspecified reactive species

Aletta M R van den Bosch,Marlijn van der Poel,Nina L Fransen,Maria C J Vincenten,Anneleen M Bobeldijk,Aldo Jongejan,Hendrik J Engelenburg,Perry D Moerland,Joost Smolders,Inge Huitinga,Jörg Hamann

View all publications

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