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リコンビナントRabMAb

Anti-C1QA 抗体 [EPR2980Y] (ab76425)

Submit a review Submit a question References (2)
Western blot - Anti-C1QA antibody [EPR2980Y] (ab76425)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR2980Y] (ab76425)
  • Anti-C1QA antibody [EPR2980Y] (ab76425)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2980Y] to C1QA
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-C1QA antibody [EPR2980Y]
    C1QA 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR2980Y] to C1QA

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt (Intra) or IP

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide within Human C1QA aa 200-300 (C terminal). The exact sequence is proprietary.

  • ポジティブ･コントロール

    • Human plasma lysate; human tonsil tissue.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab76425の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.
特記事項
WB
1/500 - 1/1000. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary.
追加情報
Is unsuitable for Flow Cyt (Intra) or IP.

ターゲット情報

  • 機能

    C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.

  • 関連疾患

    Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis.

  • 配列類似性

    Contains 1 C1q domain.
    Contains 1 collagen-like domain.

  • 翻訳後修飾

    O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups.

  • 細胞内局在

    Secreted.
  • Information by UniProt

  • 参照データベース

    • 別名

      • C1qa antibody
      • C1QA_HUMAN antibody
      • Complement C1q subcomponent subunit A antibody
      • Complement component 1 q subcomponent A chain antibody
      • Complement component 1 q subcomponent alpha polypeptide antibody
      • Complement component C1q A chain antibody
      see all

    画像

    • Western blot - Anti-C1QA antibody [EPR2980Y] (ab76425)
      Western blot - Anti-C1QA antibody [EPR2980Y] (ab76425)
      Anti-C1QA antibody [EPR2980Y] (ab76425) at 1/1000 dilution + human plasma lysate at 10 µg

      Secondary
      goat anti-rabbit HRP at 1/1000 dilution

      Predicted band size: 26 kDa
      Observed band size: 26 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR2980Y] (ab76425)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C1QA antibody [EPR2980Y] (ab76425)

      ab76425 at 1/100 dilution staining C1q in human tonsils by Immunohistochemistry, Paraffin-embedded tissue.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-C1QA antibody [EPR2980Y] (ab76425)
      Anti-C1QA antibody [EPR2980Y] (ab76425)

    参考文献 (2)

    ab76425 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab76425 は 2 報の論文で使用されています。

    • Vadászi H  et al. Competitive inhibition of the classical complement pathway using exogenous single-chain C1q recognition proteins. J Biol Chem 298:102113 (2022). PubMed: 35690144
    • Duggan SP  et al. siRNA Library Screening Identifies a Druggable Immune-Signature Driving Esophageal Adenocarcinoma Cell Growth. Cell Mol Gastroenterol Hepatol 5:569-590 (2018). PubMed: 29930979

    レビューと Q&A

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