Anti-C1QA 抗体 (ab155052)
Key features and details
- Rabbit polyclonal to C1QA
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-C1QA antibody
C1QA 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to C1QA -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 13-225 of Human C1QA (UniProt ID: P02745).
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ポジティブ･コントロール
- HepG2 and mouse spleen whole cell lysates; HeLa cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab155052の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 26 kDa.
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| ICC/IF |
1/100 - 1/1000.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 26 kDa. |
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ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes. -
関連疾患
Defects in C1QA are the cause of complement component C1qA deficiency (C1QAD) [MIM:120550]. A rare defect resulting in C1 deficiency and impaired activation of the complement classical pathway. C1 deficiency generally leads to severe immune complex disease with features of systemic lupus erythematosus and glomerulonephritis. -
配列類似性
Contains 1 C1q domain.
Contains 1 collagen-like domain. -
翻訳後修飾
O-linked glycans consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 712 Human
- Entrez Gene: 12259 Mouse
- Omim: 120550 Human
- SwissProt: P02745 Human
- SwissProt: P98086 Mouse
- Unigene: 632379 Human
- Unigene: 439957 Mouse
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別名
- C1qa antibody
- C1QA_HUMAN antibody
- Complement C1q subcomponent subunit A antibody
see all
画像
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Western blot - Anti-C1QA antibody (ab155052)Anti-C1QA antibody (ab155052) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 26 kDa
12% SDS-PAGE. -
Western blot - Anti-C1QA antibody (ab155052)Anti-C1QA antibody (ab155052) at 1/1000 dilution + mouse spleen whole cell lysate at 50 µg
Predicted band size: 26 kDa
12% SDS-PAGE. -
Immunocytochemistry/ Immunofluorescence - Anti-C1QA antibody (ab155052)Immunofluorescent analysis of methanol-fixed HeLa cells labeling C1QA with ab155052 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab155052 は 2 報の論文で使用されています。
- Györffy BA et al. Synaptic mitochondrial dysfunction and septin accumulation are linked to complement-mediated synapse loss in an Alzheimer's disease animal model. Cell Mol Life Sci 77:5243-5258 (2020). PubMed: 32034429
- Barrios AA et al. Inefficient and abortive classical complement pathway activation by the calcium inositol hexakisphosphate component of the Echinococcus granulosus laminated layer. Immunobiology 224:710-719 (2019). PubMed: 31178241